Türkiyede Yetişen Phedimus stoloniferus Türünün Uçucu Kimyasal Bileşimi ve Biyoaktivite Değerlendirilmesi

Abstract

Bu araştırmada, Türkiye'de yetişen Phedimus stoloniferus'un (Crassulaceae) esansiyel yağında (EO), SPME ve n-hekzan özütünün SPME'sindeki uçucu organik bileşiklerin (VOC) GC-FID/MS ile analiz edilmiştir. P. stoloniferus'un EO'daki uçucu bileşiklerin ana kimyasal sınıfını karboksilik asitler oluşturur (%50.2).  P. stoloniferus'un SPME ve heksan özütlerinin ve SPME’leri, ana bileşenlerinin aromatik bileşikler (sırasıyla %51.9 ve %65.9) olduğunu gösterdi.  P. stoloniferus'tan elde edilen EO, SPME ve  heksan özütlerinin SPME'sindeki  ana bileşikler sırasıyla n-Heksadekanoik asit (%34.0), 1-metil-4-etilbenzen (%20.2) ve mesitilen (%28.8)’dir. P. stoloniferus'dan elde edilen esansiyel yağlarda ve çözücü (n-heksan, metanol ve su) özütlerinde antioksidan ve tirozinaz inhibisyonları da incelenmiştir. P. stoloniferus'un metanol özütünde antioksidan aktivite en yüksek olarak (TEAC: 1.23± 0.04) tespit edilmişken, en yüksek tirosinaz enzimi inhibisyonu ise sulu özütte (IC₅₀: 0.168 ± 0.018) gözlendi.

Keywords

• Crassulaceae,Phedimus stoloniferus,SPME,GC-FID/MS,Antioksidan,Tirozinaz İnhibisyonu

Bioactivity Evaluation and Volatile Chemical Composition of Phedimus stoloniferus Species Grown in Turkey

Abstract

In this research, volatile organic compounds (VOCs) in the essential oil (EO), SPME and SPME of n-hexane extract of Phedimus stoloniferus (Crassulaceae) grown in Turkey were determined by GC-FID/MS. Major chemical class for the volatile compounds in the EO of P. stoloniferus is carboxylic acids (50.2%). SPME and SPME of the hexane extracts of P. stoloniferus revealed aromatic compounds (51.9% and 65.9%, respectively) as the main constituents. n-Hexadecanoic acid (34.0%), 1-methyl-4-ethylbenzene (20.2%), and mesitylene (28.8%) were the major compounds in the EO, SPME and SPME of hexane extract obtained from P. stoloniferus, respectively.  Antioxidant and tyrosinase inhibitions of the essential oil and n-hexane, methanol, and water extracts obtained from P. stoloniferus were also studied. While the antioxidant activity was determined to be highest in methanol extract (TEAC: 1.23±0.04), tyrosinase enzyme inhibition was highest in the aqueous extract (IC₅₀: 0.168±0.018) of P. stoloniferus.

Keywords

• Crassulaceae,Phedimus stoloniferus,SPME,GC-FID/MS,Antioxidant,Tyrosinase Inhibition

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