Türkiyede Yetişen Phedimus stoloniferus Türünün Uçucu Kimyasal Bileşimi ve Biyoaktivite Değerlendirilmesi

Year 2019, , 585 - 593, 26.12.2019

Nuri Yıldırım

https://doi.org/10.35193/bseufbd.633654

Abstract

Bu
araştırmada, Türkiye'de yetişen Phedimus stoloniferus'un (Crassulaceae)
esansiyel yağında (EO), SPME ve n-hekzan özütünün SPME'sindeki uçucu organik
bileşiklerin (VOC) GC-FID/MS ile analiz edilmiştir. P. stoloniferus'un
EO'daki uçucu bileşiklerin ana kimyasal sınıfını karboksilik asitler oluşturur
(%50.2).  P. stoloniferus'un SPME
ve heksan özütlerinin ve SPME’leri, ana bileşenlerinin aromatik bileşikler
(sırasıyla %51.9 ve %65.9) olduğunu gösterdi. 
P. stoloniferus'tan elde edilen EO, SPME ve  heksan özütlerinin SPME'sindeki  ana bileşikler sırasıyla n-Heksadekanoik asit
(%34.0), 1-metil-4-etilbenzen (%20.2) ve mesitilen (%28.8)’dir. P. stoloniferus'dan
elde edilen esansiyel yağlarda ve çözücü (n-heksan, metanol ve su) özütlerinde
antioksidan ve tirozinaz inhibisyonları da incelenmiştir. P. stoloniferus'un
metanol özütünde antioksidan aktivite en yüksek olarak (TEAC: 1.23± 0.04)
tespit edilmişken, en yüksek tirosinaz enzimi inhibisyonu ise sulu özütte (IC₅₀:
0.168 ± 0.018) gözlendi.

Keywords

Crassulaceae, Phedimus stoloniferus, SPME, GC-FID/MS, Antioksidan, Tirozinaz İnhibisyonu

Bioactivity Evaluation and Volatile Chemical Composition of Phedimus stoloniferus Species Grown in Turkey

Abstract

In this research, volatile organic compounds (VOCs)
in the essential oil (EO), SPME and SPME of n-hexane extract of Phedimus
stoloniferus (Crassulaceae) grown in Turkey were determined by GC-FID/MS.
Major chemical class for the volatile compounds in the EO of P. stoloniferus
is carboxylic acids (50.2%). SPME and SPME of the hexane extracts of P.
stoloniferus revealed aromatic compounds (51.9% and 65.9%, respectively) as
the main constituents. n-Hexadecanoic acid (34.0%), 1-methyl-4-ethylbenzene
(20.2%), and mesitylene (28.8%) were the major compounds in the EO, SPME and
SPME of hexane extract obtained from P. stoloniferus, respectively.  Antioxidant and tyrosinase inhibitions of the
essential oil and n-hexane, methanol, and water extracts obtained from P.
stoloniferus were also studied. While the antioxidant activity was
determined to be highest in methanol extract (TEAC: 1.23±0.04), tyrosinase
enzyme inhibition was highest in the aqueous extract (IC₅₀:
0.168±0.018) of P. stoloniferus.

Keywords

Crassulaceae, Phedimus stoloniferus, SPME, GC-FID/MS, Antioxidant, Tyrosinase Inhibition

References

• [1] Chamberlain, D.F., In: Davis, P.H. (ed.) Sedum L., In Flora of Turkey and the East Aegean Islands, At the Edinburgh University Press: Edinburgh, Scotland. (1972). 4, 224-244.
• [2] Alpınar, K., Sedum L. (2012). In Türkiye Bitkileri Listesi/Damarlı Bitkiler, Güner A., Aslan S., Ekim T., Vural M., Babaç M.T., Eds: Nezahat Gökyiğit Botanik Bahçesi ve Flora Araştırmaları Derneği Yayını: Istanbul, Turkey, 384-386.
• [3] Uzunhisarcıklı, M.E, (2012). Scrophularia. In: Türkiye Bitkileri Listesi (Damarlı Bitkiler). eds: Güner, A, Aslan, S, Ekim, T, Vura,l M, Babaç, MBT, Nezahat Gökyiğit Botanik Bahçesi ve Flora Araştırmaları Derneği Yayını, İstanbul.
• [4] Baytop T. (1999). Therapy with Medicinal Plants in Turkey-Past and Present, second edition, Istanbul, Nobel Publishers, 480.
• [5] Zeybek, N., Zeybek, U. (1994). Farmasotik Botanik, Izmir, Turkey, Ege University Press. 390.
• [6] Ertaş, A., Boğa, M., Yılmaz, A.M., Yeşil, Y., Haşimi, N.M., Kaya, Ş.M., Temel, H., Kolak, U. (2014). Chemical compositions by using LC-MS/MS and GC-MS and biological activities of Sedum sediforme (Jacq.). Journal Agric Food Chemistry, 62, 4601-4609.
• [7] Yaylı, N., Yasar, A., Iskender, N.Y., Yaylı, N., Cansu, T.B., Coskuncelebi, K., Karaoğlu, S.A. (2010). Chemical constituents and antimicrobial activities of the essential oils from Sedum allidum var. bithynicum and S. spurium grown in Turkey. Pharmaceutical Biology, 48, 191-194.
• [8] Mesicek N., Perpar M. (1973). Essential oils from the orpine Sedum maximum. Farmacevtski Vestnik, 24, 123-124.
• [9] Romojaro, A., Botella, M.A,. Obon, C., Pretel, M.T. (2013). Nutritional and antioxidant properties of wild edible plants and their use as potential ingredients in the modern diet. International Journal of Food Sciences and Nutrition, 64, 944-952.
• [10] Mavi, A., Terzi, Z., Ozgen, U., Yıldırım, A., Coskun, M. (2004). Antioxidant properties of some medicinal plants: Prangos ferulacea (Apiaceae), Sedum sempervivoides (Crassulaceae), Malva neglecta (Malvaceae), Cruciata taurica (Rubiaceae), Rosa pimpinellifolia (Rosaceae), Galium verum subsp. verum (Rubiaceae), Urtica dioica (Urticaceae). Biological and Pharmaceutical Bulletin, 27, 702-705.
• [11] Stankovic, M., Radojevic, I., Curcic, M., Vasic, S., Topuzovic, M., Comic, L., Markovic, S. (2012). Evaluation of biological activities of goldmoss stonecrop (Sedum acre L.). Turkish Journal of Biology, 36, 580-588.
• [12] Gendaram, O., Choi, Y.H., Kim, Y.S., Ryu, S.Y. (2011). Anti-oxidative and antibacterial constituents from Sedum hybridum. Natural Product Sciences, 17, 279-284.
• [13] Renda, G., Özel, A., Barut, B., Korkmaz, B., Yaylı, N. (2018). In Vitro protective and enzyme inhibitory effects of Crataegus microphylla extracts against oxidative damage. Turkish Journal of Pharmaceutical Sciences, 15, 77-84.
• [14] Yaylı, N., Fandaklı, S., Korkmaz, B., Barut, B., Renda, G., Erik, İ. (2018). Biological evaluation (antimicrobial, antioxidant, and enzyme inhibitions), total phenolic content and volatile chemical compositions of Caucasalia macrophylla (M. Bieb.) B. Nord. (Asteraceae). Journal of Essential Oil Bearing Plants, 21(5), 1359-1373.
• [15] Renda, G., Tevek, F., Korkmaz, B., Yaylı, N. (2017). Comparison of the Alchemilla L. samples fromturkish herbal market with the european pharmacopoeia 8.0. FABAD Journal of Pharmaceutical Sciences, 42(3), 167-177.
• [16] Renda, G., Kalaycı, Y., Korkmaz, B., Karaoğlu, Ş.A., Yaylı, N. (2017). Chemical composition and antimicrobial activity of the essential oils of Five Scrophularia L. species from Turkey. Records of Natural Products, 11,521-531.
• [17] Yaylı, N., Çelik, G., Korkmaz, B., Çoşkunçelebi, K., Karaoğlu, Ş.A. (2016). Altitude variation in the composition of essential oils fatty acid methyl esters and antimicrobial activities of two subspecies of Primula vulgaris grown in Turkey. Natural Product Communications, 11(10), 1505-1510.
• [18] Üçüncü, O., Karataş, Ş. M., Baltacı, C., Karaköse, M., Türkuçar, S. A. (2019). Volatile constituents and biological properties of essential oils from aerial parts of Gentiana gelida BIEB. Journal of Oleo Science, 68 (10), 1011-1017.
• [19] Akar, Z., Burnaz, N. A. (2019). A new colorimetric method for CUPRAC assay with using of TLC plate. LWT, 112, doi:10.1016/j.lwt.2019.05.110, 1-7.
• [20] Adams, R.P. (1995). Identification of essential oil components by gas chromatography/mass spectrometry. Allured Publishing Corporation, Carol Stream, IL.
• [21] Apak, R., Guclu, K., Ozyürek, M., Karademir, S.E. (2004). Novel total antioxidant capacity index for dietary polyphenols and vitamins C and E, using their cupric ion reducing capability in the presence of neocuproine: CUPRAC method. Journal Agric Food Chemistry, 52(26), 7970-7981.
• [22] Masuda, T., Yamashita, D., Takeda, Y., Yonemori, S. (2005). Screening for tyrosinase inhibitors among extracts of seashore plants and identification of potent inhibitors from Garcinia subelliptica. Journal Bioscience, Biotechnology and Biochemistry, 69, 197-201.
• [23] Chen, Y.S., Liou, H.C., Chan, C.F. (2013). Tyrosinase inhibitory effect and antioxidative activities of fermented and ethanol extracts of Rhodiola rosea and Lonicera japonica. The Scientific World Journal, volume 2013, Article ID 612739, 5 pages.