Plasma Modification of 3D Hydrogels for Cell Culture Applications

Year 2017, Volume: 13 Issue: 1, 105 - 112, 30.03.2017

Emel Sokullu

https://doi.org/10.18466/cbayarfbe.302649

Abstract

The aim of this study was to
examine plasma effect on to the different structures including 2D glass slides
and 3D treated Gelma hydrogels with an increased range of biodegradation rates
for more rapid in vivo biodegradation in tissue engineering and bioactive
factor delivery applications. Considering
the known excellent biocompatibility of GELMA, we envision that this hydrogel
material could be potentially used in tissue engineering and for the purpose of
directing the growth and migration of cells. Another dimension of this study is plasma patterning of
the substrates. This method has
been employed to form diverse networks of different cell types for
investigations involving migration, signaling, tissue formation, and the
behavior and interactions of neurons arraigned in a network. As a result of these, we
investigated whether the swelling behavior, degradation profiles, and cell
proliferation properties of photocrosslinked hydrogels formed by methacrylated
and plasma treated are tunable by varying the degree of plasma conditions. The
potential applicability of these photocrosslinked GELMA hydrogels as a carrier
of embryonic stem cells (ESCs) was also examined in vitro.

Keywords

Plasma treatment, hydrogels, 3D Cell Culture, embryonic stem cells (ESCs)

References

• [1] Y. Hong, H.Q. Song, Y.H. Gong, Z.W. Mao, C.Y. Gao, J.C. Shen, “Covalently crosslinked chitosan hydrogel: properties of in vitro degradation and chondrocyte encapsulation” Acta Biomater., (2007), 3:23.