Development of Embryonic Chick Liver and Distribution of eNOS, iNOS, Laminin α1

Year 2017, Volume: 13 Issue: 2, 311 - 318, 23.06.2017

Fatih Çöllü Beyhan Gürcü

https://doi.org/10.18466/cbayarfbe.305213

Abstract

At the
embryonic development, signal transduction pathways, genetic factors,
involvements between nucleus and cytoplasm, environmental factors, cell-cell
and cell-matrix interactions have important roles. It has been known that the
cells are regulating the Extracellular Matrix (ECM) synthesis, degradation and
reshaping events, also it has been known that the Nitric Oxide is an important
molecule for cellular communication and have effects on ECM molecule
distribution by reacting with ECM molecules. 
In this reason, the purpose of our study is detecting the correlation of
reactive nitrogen species with a glycosylated molecule laminin α1. In this
experiment, Leghorn type SPF (Specific Pathogen Free) embryonic chick eggs has
been used. Embryos are collected at 5^th, 6^th and 7^th
days at incubation and taken into the 10% neutral buffered formalin solution. The liver tissues
that dissected from embryos are fixated at second time. After 24 hours, it has
been subjected to the routine paraffin embedding method and embedded to
paraffin. From the 5µm sections, immunohistochemistry for eNOS, iNOS and
Laminin α1 distribution, and for general histologic evaluation,
Haematoxylene-Eosin stains has been applied. eNOS and iNOS immunoreactivity has
been observed at peripheral zone of developing liver tissue that
epithelial-mesenchymal transition takes place. It has been determined that
immunoreactivity was minimal in 5^th day, increasing with the days
progressed and have highest at 7^th day. Also, eNOS staining has been
more powerful than iNOS staining. Laminin immunoreactivity has been similar at
all developmental stages, but relatively, has been showed lesser staining.
Particularly, the presence at the zones of cell differentiation has been
noteworthy.

Keywords

eNOS, iNOS, Karaciğer gelişimi, Laminin, Piliç embriyosu, Liver development, Chick embryo

References

• Meesters, D.M., et al., Deficiency of inducible and endothelial nitric oxide synthase results in diminished bone formation and delayed union and nonunion development. Bone, 2016. 83: p. 111-118.