Genotoxic Effects of Aluminium Acetate by Micronucleus Assay

Year 2017, Volume: 13 Issue: 4, 919 - 923, 29.12.2017

Yasin Eren , Dilek Akyıl , Arzu Özkara

Abstract

Aluminium, the mostly used element in the
world, and its usage is increasing in many applications. Aluminium acetate (AA)
has been used in many therapeutical applications, too. Because of widely usage
of AA in medicine, its safety must be clearly defined. This research evaluated
the genotoxic effects of AA. It has been known that aluminium had very
important toxic effect in long time exposures. It was aimed to determine if AA
had same genotoxic effects or not. Genotoxic effects of AA were determined with
human lymphocytes MN assay. Control, Negative control (1% DMSO), positive
control (MMC, 0.20 µg/ml), 1250, 2500, 5000, 10000, and 15000 ppm doses of AA
were used in this assay. MNBC % and NDI values of AA were compared with
negative control group. 15000 ppm of AA were observed as EC50 concentration
that reduced the mitotic index about 50 %. As a result of MNBC %,  there was statistically significant increase
in MN frequency at 5000 and 10000 ppm doses. 
Dose-dependent cytotoxicity were obtained from NDI study. The highest MN
frequency and the lowest NDI frequency were obtained from 10000 ppm at 48 h
treatment while the lowest MN and the highest NDI frequencies were determined
in 1250 ppm at 24 h treatment. This research showed that used concentrations
did not produce significant MN frequency but significantly reduced the NDI
value when compared to MMC. 

Keywords

Aluminium acetate, MN assay, MNBC, NDI

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