Ethanol Extract of Pinus nigra ssp. pallasiana var. şeneriana Inhibits Human Breast Cancer Cell Viability through Induction of Apoptosis

Year 2018, Volume: 14 Issue: 1, 35 - 40, 30.03.2018

Harika Atmaca , Çisil Çamlı , Seda Sert

https://doi.org/10.18466/cbayarfbe.334883

Cited By: 3

Abstract

Novel natural compounds became the new promising source for alternative
drug production for cancer treatment. Several studies have reported that
different species of pine genus show cytotoxic activities against various
cancer cell lines. In the present study, the possible cytotoxic and apoptotic
effects of the endemic Pinus nigra ssp. pallasiana
var. şeneriana (PS) extract in
MCF-7 breast cancer cells were evaluated. Cell viability was determined via XTT assay and verified by live/dead
viability/cytotoxicity assay. Apoptotic effect was investigated by
mitochondrial membrane potential (MMP) assay. Expression of apoptosis related
proteins after treatment with PS extract was detected by apoptosis array. PS
extract treatment reduced the viability of MCF-7 cells in a time and
concentration-dependent manner. Apoptosis was induced and MMP was decreased in
MCF-7 cells by 48 h treatment. Bcl-2, cIAP-1,
cIAP-2, Survivin and XIAP protein levels
were reduced by 2.8-, 3.2-, 2.8-, 2.5- and 2.2- fold, and Bad, Bax, Cytochrome
c and SMAC/Diablo protein levels were induced by 4.2-, 3.0-, 3.6-, and 4.0-
fold in MCF-7 cells by PS extract treatment. This report describes the
cytotoxic and apoptotic effects of the extract of endemic Pinus species on
MCF-7 cells for the first time. Although
the isolation and structure elucidation of the active compounds is needed to be
performed in the further studies, it is likely that PS extract may be a
potential candidate for the development of novel therapeutic anticancer
compounds for breast cancer.

Keywords

Apoptosis, breast cancer, cytotoxicity, endemic, MCF-7, Pinus nigra

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