A Novel Biosensor Based on Laccase for The Detection of Catechol

Year 2020, Volume: 16 Issue: 1, 9 - 14, 27.03.2020

Zehra Ölçer

Abstract

Phenolic compounds are broadly used in petrol industrial production
processes and thus they are hazardous for ground and surface water and
environment. Many of them have harmful effects on plants, animals and human
health. Laccases can oxide phenol and phenolic compounds. Biosensors provide
ideal sensing systems to determine phenolic compounds. In the present paper, it
was developed novel sensor surface with modified laccase on gold electrode
which could recognize the phenolic compound catechol. The electrode arrays
integrated microfluidics have been fabricated and characterized in order to
create a sensor chip which is easy, rapid, and cheaper to produce also have
good electrochemical sensing properties. This gold electrodes were used to obtain
laccase active surfaces. Laccase was covalently bounded to self-assembled
monolayer on gold electrode via glutaraldehyde reactions. Cyclic voltammetry
and amperometry activity assays showed that the detection of catechol with
laccase modified gold surface. All of the study was carried out using automated
biosensor device. Linear range for this biosensor was 0.025 mM to 0.8 mM.

Keywords

Catechol, electrochemical biosensor, laccase immobilization

Supporting Institution

Gebze Technical University

Project Number

BAP 2018-A105-23

Thanks

The author would like to thanks the Gebze Technical University (GTU) for the provided financial support (Grant no: BAP 2018-A105-23). We gratefully acknowledge to Dr. Yıldız Uludag and Dr. Aylin Ersoy from BILGEM-TUBITAK for their contribution to the fabrication of the biochip and the sensing platform.

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