Synthesis and Anti-Biofilm Activity Studies on Novel Quinazolinone-Thiadiazole Hybrids

Year 2025, Volume: 15 Issue: 1, 170 - 174, 28.03.2025

Sevda Türk , Seyhan Ulusoy , Sevgi Karakuş , Gülgün Tınaz

https://doi.org/10.33808/clinexphealthsci.1548035

Abstract

Objective: In this study it was aimed to synthesize novel 1,3,4-thiadizole bearing 4(3H)-quinazolinone compounds, elucidate their structure and evaluate their anti-biofilm activity.
Methods: Four novel 4(3H)-quinazolinone compounds (1-4) were synthesized with a two step reaction starting from 5-bromoanthranilic acid. Their anti-biofilm activity was investigated.
Results: The final compounds’ structures were clarified by elemental analysis and spectroscopic methods (IR, 1H-NMR, 13C-NMR and MS). In the result of anti-biofilm activity studies, they possessed 26.0-30.0% biofilm formation inhibition.
Conclusion: Among the tested compounds, 6-bromo-3-{4-[5-(4-nitrophenylamino)-1,3,4-thiadiazol-2-yl]phenyl}-2-methylquinazolin-4(3H)-one formulated compound 3 was found as the most active one with 30.0% biofilm formation inhibition.

Keywords

4(3H)-Quinazolinones, 1-3-4-thiadiazoles, anti-biofilm activity, P. aeruginosa

Ethical Statement

This study didn’t need an Ethics Committee Approval.

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