Effects of oxytocin and oxytocin receptor antagonist atosiban on nociception and morphine analgesia in rats

Year 2017, Volume: 39 Issue: 4, 665 - 670, 18.12.2017

Ahmet Sevki Taskıran Ercan Ozdemir Handan Gunes Berat Erdem Gokhan Arslan

https://doi.org/10.7197/223.v39i32356.369060

Abstract

Objective: Oxytocin is a peptide-based hormone released
from the supraoptic nucleus and paraventricular nucleus in the hypothalamus and consisting of nine
amino acids. It has been shown that oxytocin may have an effect on opiate
receptors. Atosiban is a oxytocin receptor antagonist. Our aim in this study
was to investigate the effects of oxytocin and atosiban on nociception and
morphine analgesia.

Method: In our study, 48 Wistar Albino 230-260 g male rats
were used. The animals were divided into eight groups (control, 200 μg/kg
oxytocin, 3 mg/kg atosiban, oxytocin+ atosiban, 5 mg/kg morphine, morphine+
oxytocin, morphine+atosiban and morphine+ oxytocin+ atosiban). Serum
physiologic to the control group, oxytocin and atosiban were given
intraperitoneally (i.p.) at the indicated doses to the other groups. Morphine
was administered subcutaneously (s.c.). Analgesic effects were assessed by hot
plate and tail flick analgesia tests. The resulting analgesic effect was
measured and recorded at the 15th, 30th, 60th, 90th and 120th minutes.
Assessment of analgesic effect was formulated as % analgesia (MPE) (% analgesia
= 100x [post drug reaction time-pre drug reaction time]/ [cut off time-pre drug
reaction time]).

Results: Oxytocin showed analgesic activity (p<0,05). Atosiban showed
hyperalgesic activity and decreased the analgesic activity of oxytocin when
given with oxytocin (p<0,05). Oxytocin increased the analgesic activity of
morphine (p<0.05). In addition, although atosiban did not alter the
analgesic activity of morphine, morphine analgesia increased by oxytocin was
reduced (p<0.05).

Conclusions: According to these results, it can be said that although atosiban does
not change on the analgesic effect of morphine alone, it blocks the effect of
oxytocin on morphine analgesia.

Keywords

Atosiban, oxytocin, nociception, morphine analgesia, analgesia tests

Sıçanlarda oksitosin ve oksitosin reseptör antagonisti atosibanın nosisepsiyon ve morfin analjezi üzerine etkileri

Abstract

Amaç: Oksitosin, hipotalamusta supraoptik çekirdek ve
paraventrikülerçekirdeklerden salınan, dokuz amino asitten oluşan, peptid
yapılı bir hormondur. Oksitosin'in opiyat reseptörleri üzerinde etkili
olabileceği gösterilmiştir. Atosiban, oksitosinin reseptör antagonistidir. Bu
çalışmada amacımız, oksitosin ve atosibanın nosisepsiyon ve morfin analjezisi
üzerine etkilerini araştırmaktır.

Yöntem:
Çalışmamızda 48 Wistar Albino 230-260 g erkek sıçan kullanıldı. Hayvanlar
kontrol, 200 μg/kg oksitosin, 3 mg/kg atosiban, oksitosin+atosiban, 5 mg/kg
morfin, morfin+oksitosin, morfin+ atosiban ve morfin+oksitosin+atosiban olmak
üzere sekiz gruba ayrıldı. Kontrol grubuna serum fizyolojik, diğer gruplara
belirtilen dozlarda oksitosin ve atosiban intraperitoneal (i.p.) olarak
verildi. Morfin, subkütan (s.c.) yoldan verildi. Analjezik etkinliktail flick
ve hot plate analjezi testleriyle değerlendirildi. Ortaya çıkan analjezik etki
15., 30., 60., 90. ve 120. dakikalarda ölçüldü ve kaydedidi. Analjezik etkinin
değerlendirilmesi % analjezi (MPE) (% analjezi = 100x [ilaç sonrası tepki
süresi - ilaç öncesi tepki süresi] / [test kesme süresi - ilaç öncesi tepki
süresi] şeklinde formüle edildi.

Bulgular:
Oksitosin analjezik etkinlik gösterdi (p <0,05). Atosiban, hiperaljezik
aktivite gösterdi ve oksitosin ile birlikte verildiğinde oksitosin'in analjezik
etkinliğini azalttı (p <0,05). Oksitosin morfinin analjezik etkinliğini
arttırdı (p<0,05). Buna ek olarak, atosiban morfinin analjezik etkinliğini değiştirmese de,
oksitosin ile artmış morfin analjezisini azalttı (p <0.05).

Sonuç:
Bu sonuçlara göre, atosibanın tek başına morfinin analjezik etkisini
değiştirmemesine rağmen, oksitosinin morfin analjezi üzerindeki etkisini bloke
ettiği söylenebilir.

Keywords

Atosiban, oksitosin, nosisepsiyon, morfin analjezi, analjezi testleri

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