Effect of captopril on the oxidative damage caused by pentylenetetrazole in the SHSY-5Y human neuroblastoma cell line

Year 2020, Volume: 42 Issue: 4, 479 - 483, 31.12.2020

Murat Doğan Mustafa Karademir

https://doi.org/10.7197/cmj.830835

Cited By: 1

Abstract

Objective: Epilepsy is a crucial brain disorder that seizures could cause a neuronal loss in the hippocampus. Oxidative stress has an important role in the pathology of this way. The aim of this study was to investigate the neuroprotective effect of captopril, on pentylenetetrazole (PTZ) induced epileptic seizures in SH-SY5Y cell line. 

Method: In this XTT cell viability assay, captopril was performed in vitro SH-SY5Y cell culture to evaluate PTZ-induced neurotoxicity. Tissue TOS concentrations at the cell supernatants were quantified with the automated assay method. Hydrogen peroxide was used for the calibration of the assay.

Results: When XTT cell viability results were evaluated, captopril did not affect neuronal viability in SH-SY5Y cell line. Moreover, captopril did not have significant effect on TOS levels (***P>0. 001)

Conclusions: Results showed that, captopril did not have neuroprotective properties in SH-SY5Y cell line after PTZ-induced neurotoxicity.

Keywords

Neurotoxicity, captopril, PTZ

Supporting Institution

Sivas Cumhuriyet University, School of Medicine, CUTFAM Research Center, Sivas, Turkey

Thanks

The authors would like to thank the Sivas Cumhuriyet University, School of Medicine, CUTFAM Research Center, Sivas, Turkey, for providing the necessary facilities to conduct this study.

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