Larvicidal and Ovicidal Properties of Essential Oils Derived from Origanum minutiflorum and Salvia dorystoechas against the Aedes aegypti

Year 2024, Volume: 8 Issue: 1, 18 - 23, 30.06.2024

Zeynep Nur Gültekin Şevval Kahraman Hüseyin Çetin

https://doi.org/10.31594/commagene.1445581

Abstract

This study assessed the larvicidal and ovicidal effects of essential oils extracted from the above ground of Origanum minutiflorum and Salvia dorystoechas, belonging to the Lamiaceae plant family, on Aedes aegypti mosquito larvae and eggs. The research utilized essential oil concentrations ranging from 1 to 100 ppm. Larvicidal activity was evaluated 6 hours post-exposure and subsequently at 24-hour intervals over a period of 5 days while ovicidal activity was monitored every 2 days for a total of 10 days. Findings indicated that both essential oils demonstrated significant larvicidal effects at 50 and 100 ppm concentrations against Ae. aegypti larvae. In contrast, ovicidal effects were not observed to be statistically significant. Throughout the experiment, O. minutiflorum essential oil consistently showed higher larvicidal potency compared to S. dorystoechas. Therefore, while essential oils from both O. minutiflorum and S. dorystoechas effectively target Ae. aegypti larvae, especially at elevated concentrations, they did not exhibit ovicidal properties.

Keywords

Insecticide, lamiaceae, mosquito, toxicity

Ethical Statement

No necessary

Project Number

-

Origanum minutiflorum ve Salvia dorystoechas'tan Elde Edilen Uçucu Yağların Aedes aegypti’ ye Karşı Larvisidal ve Ovisidal Özellikleri

Abstract

Bu çalışmada, Lamiaceae bitki ailesine ait Origanum minutiflorum ve Salvia dorystoechas'ın toprak üstü kısımlarından elde edilen uçucu yağların Aedes aegypti sivrisinek larvaları ve yumurtaları üzerindeki larvasidal ve ovisidal etkileri değerlendirilmiştir. Araştırmada 1 ila 100 ppm arasında değişen uçucu yağ konsantrasyonları kullanılmıştır. Larvasidal aktivite maruziyetten 6 saat sonra ve daha sonra 5 günlük bir süre boyunca 24 saatlik aralıklarla değerlendirilirken, ovisidal aktivite toplam 10 gün boyunca her 2 günde bir izlenmiştir. Bulgular, her iki uçucu yağın da Ae. aegypti larvalarına karşı 50 ve 100 ppm konsantrasyonlarında önemli larvasidal etkiler gösterdiğini ortaya koymuştur. Buna karşılık, ovisidal etkilerin istatistiksel olarak anlamlı olmadığı gözlenmiştir. Araştırma süresince, O. minutiflorum uçucu yağı S. dorystoechas'a kıyasla sürekli olarak daha yüksek larvisidal etki göstermiştir. Bu nedenle, hem O. minutiflorum hem de S. dorystoechas uçucu yağları, özellikle yüksek konsantrasyonlarda Ae. aegypti larvalarını etkili bir şekilde hedef alırken, ovisidal özellikler sergilememiştir.

Keywords

İnsektisit, Lamiaceae, sivrisinek, toksisite

Project Number

-

References

• Ahebwa, A., Hii, J., Neoh, K. B., & Chareonviriyaphap, T. (2023). Aedes aegypti and Aedes albopictus (Diptera: Culicidae) ecology, biology, behaviour, and implications on arbovirus transmission in Thailand. One Health, 100555. https://doi.org/10.1016/j.onehlt.2023.100555
• Ahmed, Q., Agarwal, M., Al-Obaidi, R., Wang, P., & Ren, Y. (2021). Evaluation of aphicidal effect of essential oils and their synergistic effect against Myzus persicae (Sulzer) (Hemiptera: Aphididae). Molecules, 26(10), 3055. https://doi.org/10.3390/molecules26103055
• Arserim, S. K., Cetin, H., Yildirim, A., Limoncu, M. E., Cinbilgel, I., Kaya, T., Ozbel, Y., & Balcioglu, I. C. (2021). The toxicity of essential oils from three Origanum species against head louse, Pediculus humanus capitis. Acta Parasitologica, 66, 1003-1011. https://doi.org/10.1007/s11686-021-00370-y
• Asgarian, T. S., Vatandoost, H., Hanafi-Bojd, A. A., & Nikpoor, F. (2023). Worldwide status of insecticide resistance of Aedes aegypti and Aedes albopictus, vectors of arboviruses of Chikungunya, Dengue, Zika and Yellow Fever. Journal of Arthropod-Borne Diseases, 17(1), 1. https://doi.org/10.18502%2Fjad.v17i1.13198
• Becker, N., Petric, D., Zgomba, M., Boase, C., Madon, M., Dahl, C., & Kaiser, A. (2010). Mosquitoes and Their Control. Springer Science & Business Media.
• Cetin, H., & Yanikoglu, A. (2006). A study of the larvicidal activity of Origanum (Labiatae) species from southwest Turkey. Journal of Vector Ecology, 31(1), 118-122. https://doi.org/10.3376/1081-1710(2006)31[118:ASOTLA]2.0.CO;2
• Cetin, H., Tufan-Cetin, O., Turk, A. O., Tay, T., Candan, M., Yanikoglu, A., & Sumbul, H. (2012). Larvicidal activity of some secondary lichen metabolites against the mosquito Culiseta longiareolata Macquart (Diptera: Culicidae). Natural Product Research, 26(4), 350-355. https://doi.org/10.1080/14786411003774296
• Cetin, H., Yanikoglu, A., & Cilek, J. E. (2011). Larvicidal activity of selected plant hydrodistillate extracts against the house mosquito, Culex pipiens, a West Nile virus vector. Parasitology Research, 108, 943-948. https://doi.org/10.1007/s00436-010-2136-z
• Ebadollahi, A., Ziaee, M., & Palla, F. (2020). Essential oils extracted from different species of the Lamiaceae plant family as prospective bioagents against several detrimental pests. Molecules, 25(7), 1556. https://doi.org/10.3390/molecules25071556
• Kiran, S. R., Bhavani, K., Devi, P. S., Rao, B. R., & Reddy, K. J. (2006). Composition and larvicidal activity of leaves and stem essential oils of Chloroxylon swietenia DC against Aedes aegypti and Anopheles stephensi. Bioresource Technology, 97(18), 2481-2484. https://doi.org/10.1016/j.biortech.2005.10.003
• Koc, S., Gultekin, Z. N., Kahraman, S., Cengiz, A., Polat, B., Caliskan, C., Tufan-Cetin, O., & Cetin, H. (2024). Larvicidal and repellent effects of essential oils on the brown dog tick (Rhipicephalus sanguineus sensu lato) with description of new larval repellent activity test method. Experimental and Applied Acarology, 1-11. https://doi.org/10.1007/s10493-023-00892-2
• Koc, S., Oz, E., Aydın, L., & Cetin, H. (2012). Acaricidal activity of the essential oils from three Lamiaceae plant species on Rhipicephalus turanicus Pom. (Acari: Ixodidae). Parasitology Research, 111, 1863-1865. https://doi.org/10.1007/s00436-012-2939-1
• Komalamisra, N., Trongtokit, Y., Rongsriyam, Y., & Apiwathnasorn, C. (2005). Screening for larvicidal activity in some Thai plants against four mosquito vector species. Southeast Asian Journal of Tropical Medicine and Public Health, 36(6), 1412.
• Lobato Rodrigues, A. B., Martins, R. L., Rabelo, É. D. M., Tomazi, R., Santos, L. L., Brandão, L. B., ... & de Almeida, S. S. M. D. S. (2021). Development of nano-emulsions based on Ayapana triplinervis essential oil for the control of Aedes aegypti larvae. PLoS One, 16(7), e0254225. https://doi.org/10.1371/journal.pone.0254225
• López, V., Pavela, R., Gómez-Rincón, C., Les, F., Bartolucci, F., Galiffa, V., ... & Benelli, G. (2019). Efficacy of Origanum syriacum essential oil against the mosquito vector Culex quinquefasciatus and the gastrointestinal parasite anisakis simplex, with insights on acetylcholinesterase inhibition. Molecules, 24(14), 2563. https://doi.org/10.3390/molecules24142563
• Moein, M. R., Ghasemi, Y., Moein, S., & Nejati, M. (2010). Analysis of antimicrobial, antifungal and antioxidant activities of Juniperus excelsa M. B subsp. polycarpos (K. Koch) Takhtajan essential oil. Pharmacognosy Research, 2(3), 128. https://doi.org/10.4103%2F0974-8490.65505
• Mundim-Pombo, A.P.M., Carvalho, H.J.C., Rodrigues Ribeiro, R. Leon, M., Maria D.A., & Miglino, M.A. (2021). Aedes aegypti: egg morphology and embryonic development. Parasites Vectors 14, 531. https://doi.org/10.1186/s13071-021-05024-6
• Polat, Y. (2017). Bazı bitki uçucu yağlarının kum sineklerine karşı fumigant toksik etkisinin araştırılması. Ms Thesis in Akdeniz University, Antalya, Türkiye
• Sarma, R., Adhikari, K., Mahanta, S., & Khanikor, B. (2019). Combinations of plant essential oil based terpene compounds as larvicidal and adulticidal agent against Aedes aegypti (Diptera: Culicidae). Scientific Reports, 9(1), 9471. https://doi.org/10.1038/s41598-019-45908-3
• Ser, O., & Cetin, H. (2019). Investigation of susceptibility levels of Culex pipiens L. (Diptera: Culicidae) populations to synthetic pyrethroids in Antalya province of Turkey. Journal of Arthropod-Borne Diseases, 13(3), 243. https://pubmed.ncbi.nlm.nih.gov/31879665
• Soonwera, M. (2015). Efficacy of essential oil from Cananga odorata (Lamk.) Hook. f. & Thomson (Annonaceae) against three mosquito species Aedes aegypti (L.), Anopheles dirus (Peyton and Harrison), and Culex quinquefasciatus (Say). Parasitology Research, 114(12), 4531-4543. https://doi.org/10.1007/s00436-015-4699-1
• Sousa, V. I., Parente, J. F., Marques, J. F., Forte, M. A., & Tavares, C. J. (2022). Microencapsulation of essential oils: A review. Polymers, 14(9), 1730. https://doi.org/10.3390/polym14091730
• Touray, M., Bakirci, S., Ulug, D., Gulsen, S. H., Cimen, H., Yavasoglu, S. I., ... & Hazir, S. (2023). Arthropod vectors of disease agents: their role in public and veterinary health in Turkiye and their control measures. Acta Tropica, 106893. https://doi.org/10.1016/j.actatropica.2023.106893
• Tuberoso, C. I., Kowalczyk, A., Coroneo, V., Russo, M. T., Dessì, S., & Cabras, P. (2005). Chemical composition and antioxidant, antimicrobial, and antifungal activities of the essential oil of Achillea ligustica all. Journal of Agricultural and Food Chemistry, 53(26), 10148-10153. https://doi.org/10.1021/jf0518913
• Warikoo, R., Wahab, N., & Kumar, S. (2011). Oviposition-altering and ovicidal potentials of five essential oils against female adults of the dengue vector, Aedes aegypti L. Parasitology Research, 109, 1125-1131. https://doi.org/10.1007/s00436-011-2355-y