Micromeria myrtifolia Boiss. & Hohen. Bitkisinin Farmakolojik Potansiyelinin Araştırılması: Fenolik İçeriğinin ve Biyolojik Aktivitelerinin Belirlenmesi

Year 2024, Volume: 8 Issue: 2, 103 - 114

Ezgi Ersoy , Rümeysa Batal Yıldızhan , Mustafa Abdullah Yılmaz , Yeter Yeşil , Hasan Şahin , Esra Eroğlu Özkan , Mehmet Boğa

https://doi.org/10.31594/commagene.1576852

Abstract

Micromeria myrtifolia Boiss. & Hohen., Türkiye'nin önemli tıbbi bitkilerindendir ve ülke genelinde farklı tıbbi amaçlarla oldukça yaygın olarak kullanılmaktadır. Bu çalışmada, M. myrtifolia örnekleri Muğla, Türkiye'den toplanmış hem topraküstü kısımlarından hem de köklerden ayrı ayrı etanol ekstreleri hazırlanmıştır. Ekstrelerin fitokimyasal içeriklerini belirlemek için detaylı bir LC-MS/MS analizi yapılmıştır. Analiz sonuçlarına göre her iki ekstrenin de fenolik bileşikler açısından oldukça zengin olduğu belirlenmiştir. Her iki ekstrede de ana bileşen olarak nikotiflorin öne çıkmış ve topraküstü kısımlarından hazırlanan ekstrede 12878.31±355.44 μg/g, kök ekstresinde ise 47512.4±1311.34 μg/g nikotiflorin tespit edilmiştir. Ayrıca ekstrelerde rozmarinik asit ve kafeik asit gibi önemli fenolik asitler ile hesperidin gibi flavonoitlerin varlığı da belirlenmiştir. Ekstrelerin antioksidan aktiviteleri DPPH serbest radikal giderici, ABTS katyon radikali giderici ve CUPRAC aktivitesi metotları ile değerlendirilmiş, buna göre her iki ekstre de sentetik antioksidan bileşiklere kıyasla anlamlı antioksidan aktiviteler sergilemiştir. Bunun yanı sıra ekstreler enzim inhibisyon aktiviteleri açısından değerlendirildiğinde, ekstrelerin 2 mg/mL konsantrasyonda %73.03±0.16 (topraküstü) ve %47.06±0.41 (kök) oranında anti-glukozidaz aktivite gösterdiği tespit edilmiştir. Kök ekstresi ayrıca orta düzeyde butirilkolinesteraz inhibe edici aktivite de göstermiştir. Bu çalışma, M. myrtifolia bitkisinin kimyasal içeriği ve biyolojik aktiviteleri konusunda mevcut literatüre önemli katkılarda bulunmakla birlikte, bitkinin geleneksel ve modern tıp uygulamalarındaki yeni ve potansiyel kullanım alanlarını keşfetmeye yönelik gelecek araştırmalara da zemin hazırlamaktadır.

Keywords

Micromeria Benth., LC-MS/MS, Antioksidan, Enzim İnhibisyonu

Investigating the Pharmacological Potential of Micromeria myrtifolia Boiss. & Hohen.: Phenolic Profiling and Biological Activity Assessments

Abstract

Micromeria myrtifolia Boiss. & Hohen. is a valuable medicinal plant in Türkiye, recognized for its extensive applications across the country. In this study, plant samples were collected from Muğla, Türkiye and extracts from both the aerial parts and roots were prepared using ethanol. To elucidate their phytochemical composition, a comprehensive LC-MS/MS analysis was conducted. The results revealed that both extracts were quite rich in phenolic compounds. Notably, nicotiflorin was the major constituent in both extracts (12878.31355.44 μg/g extract in the aerial parts and 47512.41311.34 μg/g extract in the roots) along with significant phenolic acids such as rosmarinic acid and caffeic acid as well as flavonoids like hesperidin. Moreover, both extracts demonstrated substantial antioxidant activities compared to the synthetic antioxidant compounds as evaluated by DPPH free radical scavenging, ABTS cation radical scavenging, and CUPRAC activity assays. Both aerial parts and root extracts also exhibited meaningful anti-glucosidase activity with 73.030.16% and 47.060.41%, respectively, at 2 mg/mL concentration. The root extract also showed moderate butyrylcholinesterase inhibitory activity. This study contributes to the existing body of knowledge with valuable insights regarding the phytochemical profile and biological activities of M. myrtifolia, paving the way for future research aimed at exploring its medicinal properties and potential uses in traditional and modern medicine.

Keywords

Micromeria Benth, LC-MS/MS, antioxidant, enzyme inhibition

Ethical Statement

Ethics committee approval is not required for this study.

Supporting Institution

Dicle Üniversitesi

Thanks

This study is a part of Rumeysa BATAL YILDIZHAN’s master thesis from Dicle University, Faculty of Pharmacy, Analytical Chemistry Department

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