comu j. agri. fac. ÇOMÜ Ziraat Fakültesi Dergisi 2147-8384 2564-6826 Çanakkale Onsekiz Mart University 10.33202/comuagri.1387460 Zootechny (Other) Hayvansal Üretim (Diğer) Odun Çeliklerinin Mikroskobik İnceleme ve Görüntülenmesinde Farklı Boyama Tekniklerinin Kullanımı Üzerine Araştırmalar https://orcid.org/0000-0001-6897-8708 Engin Hakan CANAKKALE ONSEKIZ MART UNIVERSITY, FACULTY OF AGRICULTURE, DEPARTMENT OF HORTICULTURE https://orcid.org/0000-0003-0497-4331 Kuzucu Fatih Cem CANAKKALE ONSEKIZ MART UNIVERSITY, FACULTY OF AGRICULTURE, DEPARTMENT OF HORTICULTURE https://orcid.org/0000-0002-0012-9782 Gökbayrak Zeliha CANAKKALE ONSEKIZ MART UNIVERSITY, FACULTY OF AGRICULTURE, DEPARTMENT OF HORTICULTURE, DEPARTMENT OF HORTICULTURE 07 22 2024 12 1 108 120 11 07 2023 03 20 2024 Copyright © 2013, COMU Journal of Agriculture Faculty 2013 COMU Journal of Agriculture Faculty

Öz Bu çalışmada, asma çeliklerinden kök oluşumunun mikroskobik incelenme ve görüntülenmesinde oldukça etkili olan ve tarafımızdan geliştirilen boyama ve görüntüleme yöntemleri sunulmaktadır. Farklı boya maddeleri farklı hücre tipleri ve bileşenleriyle tepkimeye girerek kendilerine özğü renk özelliklerini ortaya çıkarırlar. Asma kesitlerinde doğal renk farklılıkları birbirine çok yakındır. Bundan dolayı asma kök oluşumunun ve dal dokularının yüzey ve iç özellikleri göstermek için konsantrasyonunu arttırmanın en iyi yolu boyamadır. Bu amaçla araştırmamızda asma çeliklerinden kök oluşumunda hücre ve doku düzeyleri üzerine ayırt edici bilgiler sağlayarak net bir tanımlama ortaya koyabilmek için farklı boyama maddeleri ve bunların farklı karışımları üzerinde çalışılmıştır. Asma kesitlerine yapılan boyama uygulamaları Toluidine blue O, Aniline blue, Safranin O, Bromophenol blue, Methyl green, Basic fuchsin, Giemsa stain, Fast green FCF ve Carmin’dir. Bu uygulamalardan Toluidine blue O, Safranin O, Bromophenol blue, Fast green FCF ve Aniline blue boyamaları hücre ve doku düzeyleri üzerine etkili sonuçlar ortaya koymuştur. Ayrıca araştırmamızda kullanılan iki çift boyama yönteminden (Safranin O+ Bromophenol blue ve Bromophenol blue+Fast green FCF) başarılı sonuçlar alınmıştır. Her iki çift boyama yöntemi de asma gövde ve kök kesitlerinin mikroskop altında incelenmesinde kullanılması tavsiye edilir. Boyanan kesitlerin yüzeyindeki renklenmelerin ve keskinliğin mikroskop altında görüntüleme ve fotoğraflamasının iyileştirilmesi için tarafımızdan ‘halka ışık’ geliştirilmiştir. Halka ışık, numunenin tamamı üzerine beyaz ışık, sarı ışık ve her iki ışık yoğunluğunu farklı oranlarda kullanarak aydınlatma olanağı sunmaktadır.

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