The Investigation of the Effects of Chlorpyrifos and 2,4-Dichlorophenoxyacetic Acid Application on Bovine Liver Catalase Activity

Year 2018, Volume: 39 Issue: 3, 615 - 620, 30.09.2018

Hasan Karadağ

https://doi.org/10.17776/csj.348453

Abstract

In
this study, it was investigated whether different concentrations of
organophosphate insecticide chlorpyrifos (CPF) and systemic herbicide
2,4-dichlorophenoxyacetic acid (2,4-D) 
on bovine liver catalase (CAT) activity cause any inhibitions or
activations. For this purpose, 25, 50, 100, 250 and 500 ppm concentrations of
CPF and 2,4-D were used. Following the applications of all tested
concentrations of the both pesticides, the CAT activity elevated. Under the
exposure of 25, 50, 100, 250 and 500 ppm concentrations, % CAT activity
increases were calculated as 10.0; 6.2; 4.6; 6.9 and 6.0 in CPF apllications,
while these increases were calculated as 13.1; 10.3; 17.0; 24.4 and 18.8 in
2,4-D applications, respectively. The present research indicated the elevations
in CAT activity with 2,4-D were higher compared to CPF. This means that 2,4-D
may have increased hydrogen peroxide production more than CPF.

Keywords

Catalase, chlorpyrifos, pesticide, 2, 4-dichlorophenoxyacetic acid

Klorpirifos ve 2,4-Diklorofenoksiasetik Asit Uygulamasının Sığır Karaciğer Katalaz Aktivitesi Üzerine Etkilerinin İncelenmesi

Abstract

Bu
çalışmada, organofosfat insektisit klorpirifos (CPF)  ve sistemik herbisit 2,4-diklorofenoksiasetik
asit (2,4-D)’nin farklı konsantrasyonlarının, sığır karaciğer katalazı (CAT)
üzerine herhangi bir inhibisyon ya da aktivasyona neden olup olmadığı araştırılmıştır.
Bu amaçla CPF ve 2,4-D’nin 25, 50, 100, 250 ve 500 ppm konsantrasyonları
kullanılmıştır. Her iki pestisitin test edilen tüm konsantrasyonlarının
uygulanmasını takiben CAT aktivitesi artmıştır. 25, 50, 100, 250 ve 500 ppm
konsantrasyonların etkisinde % CAT aktivite artışları CPF uygulamalarında
sırasıyla 10.0; 6.2; 4.6; 6.9 ve 6.0 olarak hesaplanmışken bu artışlar 2,4-D
uygulamalarında sırasıyla 13.1; 10.3; 17.0; 24.4 ve 18.8 olarak hesaplanmıştır.
Sunulan araştırma CAT aktivitesi artışlarının CPF’ye oranla 2,4-D
uygulamalarında daha yüksek olduğunu göstermektedir. Bu 2,4-D’nin hidrojen
peroksit üretimini CPF’ye göre daha fazla arttırmış olabileceği anlamına
gelmektedir.

Keywords

Katalaz, Klorpirifos, 2, 4-diklorofenoksiasetik asit, pestisit

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