Yeni bir imin bileşiğinin yapısal karakterizasyonu ve DNA bağlanma özellikleri

Year 2020, Volume: 41 Issue: 2, 407 - 412, 25.06.2020

Muhammet Köse Ozge Gungör Julide Nacaroglu Ballı Hilal Kırpık

https://doi.org/10.17776/csj.594938

Abstract

Bu çalışmada, o-vanilin ve 4-izopropilanilin
reaksiyonundan yeni bir imin bileşiği (M) sentezlenmiştir. Bileşiğin yapısı,
spektroskopik ve analitik yöntemlerle karakterize edilmiştir. Bileşiğin tekli
kristalleri elde edilerek katı hal yapısı X-ışını kırınım çalışması ile
karakterize edilmiştir. Bileşiğin UV-Vis absorpsiyon ve emisyon özellikleri
farklı çözücülerde çalışılmıştır. Ayrıca, bileşiğin DNA bağlama kabiliyeti
araştırılmış ve standart DNA bağlayıcı ajanlarla karşılaştırılmıştır. Bileşik,
spermin ve etidyum bromüre yakın bağlanma sabiti (4 x 10⁵ M^-1) sahip olduğu bulunmuştur.

Structural characterization and DNA binding properties of a new imine compound

Abstract

In this study, a new imine compound (M) was prepared from the reaction of o-vanillin and 4-isopropylaniline. The structure of the compound was characterized by the spectroscopic and analytical methods. Single crystals of the compound were grown and solid-state structure of the compound was further characterized by X-ray diffraction study. The UV-Vis absorption and emission properties of the compound were studied in different solvents. Moreover, DNA binding ability of the compound was investigated and compared with the standard DNA binding agents. The compound showed similar binding constant (4 x 105 M-1) to those spermine and ethidiumbromide.

Keywords

Imine, Absorption, Emission, X-ray, DNA binding

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