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Vitamin C-E ve Monosodyum Glutamata Bağlı Ovaryan Toksisite

Year 2014, Volume: 39 Issue: 3, 517 - 524, 22.07.2014
https://doi.org/10.17826/cutf.61415

Abstract

Amaç: Bu çalışmada oral monosodyumglutamat (MSG) verilmiş dişi sıçanlara, C ve E vitaminleri kombine olarak uygulandığında meydana gelen tesire bağlı olarak oksidatif stres durumu, folikül sayısı ve hormon düzeyi gibi değişkenlerin nasıl etkilendiklerini test etmeyi amaçladık. Materyal ve Metot: 12 haftalık dişi Wistar sıçanlarından bir kısmına sadece MSG (140mg/200 g) verildi, ikinci kısmına ise MSG ile C ve E vitaminleri kombine olarak verildi. Yirmibeş sıçan toplamda 5 gruba ayrıldı (her grup için n=5). Bu gruplardan ilk ikisi kontrol grubu ve sadece MSG verilen grup olarak belirlendiler. Son üç gruba ise MSG"ye ilave olarak farklı dozlarda C ve E vitaminleri kombine olarak verildi. Değişik C ve E dozlarına göre oluşturulan bu gruplar aşağıdaki gibidirler; üçüncü gruba MSG"ye ilave olarak 0.2 mg/g bw(vücut ağırlığı) oranında C vitamini ve 0.04 IU/g bw oranında E vitamini (MSG + CE1) verildi. Dördüncü gruba, MSG"ye ilave olarak 0.4 mg/g bw oranında C vitamini ve 0.04 IU/g bw oranında E vitamini (MSG + CE2) verildi. Beşinci gruba ise , MSG"ye ilave olarak 0.6 mg/g bw oranında C vitamini ve 0.04 IU/g bw E vitamini (MSG + CE3) verildi. Malondialdehit (MDA) analizi kolorimetrik olarak yapıldı. Folikül sayısı, hematoksileneozin boyama yöntemi kullanılmak suretiyle hispatolojik olarak belirlendi. 17&#946;-estradiol ve folikül stimüle edici hormon (FSH) düzeyleri, eliza tekniği kullanılarak belirlendi. Bulgular: MSG verilmiş tüm gruplar kontrol grubu ile karşılaştırıldıklarında, MDA düzeylerinin ve atrezik folikül sayısının istatistiksel olarak önemli oranda artmış olduğu tespit edilmiştir (P < 0.05). Sadece MSG verilen grup ile kontrol grubu karşılaştırıldıklarında; sadece MSG verilmiş grupta, FSH ve 17&#946;-estradiol hormon düzeyleri ile primer, sekonder ve graff folikülleri sayılarının anlamlı olarak düşmüş oldukları tespit edildi (P < 0.05). Sadece MSG verilmiş grup ile karşılaştırıldığında, C ve E vitaminlerinin kombine olarak verilmesinin atrezikfolikül sayısı ve MDA düzeylerini anlamlı olarak düşürdüğü tespit edilmiştir. MSG"ya ilave olarak C ve E vitaminlerinin kombine olarak verildiği durumlarda MDA düzeyleri ve atrezik folikül sayısı kontrol grubu düzeyine düşmüştür (P < 0.05). Buna göre MSG + CE2 ve MSG + CE3 grupları ile sadece MSG verilmiş grup karşılaştırıldıklarında, MSG + CE2 ve MSG + CE3 gruplarında FSH düzeyleri ile primer folikül sayısının anlamlı olarak artmış olduğu (kontrol grubu düzeyine ulaşmış olduğu) tespit edilmiştir(P > 0.05). Sadece MSG verilmiş grup ile karşılaştırıldıklarında; C ve E vitaminlerinin tüm dozlarının, 17&#946;-estradiol düzeyi ile sekonder ve graff folikül sayılarını anlamlı olarak arttırdıkları tespit edilmiştir (P < 0.05). C ve E vitaminlerinin verildiği gruplarda, 17&#946;-estradiol düzeyi ile sekonder ve graffolikül sayıları, kontrol grubunun üzerindeki düzeylere ulaşmıştır (P < 0.05). Sonuç: Bulgularımız C ve E vitamin kombinesinin MSG"ya maruz kalmaya bağlı olarak gelişen ovar

References

  • Eweka O. Histological studies of the effects of monosodium glutamate on the kidney of adult Wistar rats. Internet J Health. 2007;6:2.
  • Olney JW. Glutamate induced neuronal neurosis in the infant mouse hypothalamus. J. Neuropath Exp Neurol. 1971;30,75-90.
  • Onakewhor J, Oforofuo I, Singh S. Chronic administration of monosodium glutamate induces oligozoospermia and glycogen accumulation in Wistar rat testes. Afr J Reprod Health. 1998;2:190–7.
  • Oforofuo O, Onakewhor J, Idaewor P. The effect of chronic administration of MSG on the histology of the adult Wistar rat testes. Biosci Res Commun. 1997;9:2.
  • Metawie MAEH, Mouselly M. Antral follicular count as a predictor of ovarian respon in induction of ovulation in patients with anovulatory Infertility. Suez Canal Univ Med J. 2003;6:35-40.
  • Regan KS, Cline JM, Creasy D, Davis B, Foley GL, Lanning L, Latendresse JR, Makris, S, Morton D, Rehm S, Stebbins K. Ovarian follicular counting in the assessment of rodent reproductive toxicity. Toxicol Pathol. 2005;33:409–12.
  • Agarwal A, Mellado AA, Premkumar BJ, Shaman A, Gupta S. The effects of oxidative stress on female reproduction: a review. Reprod Biol Endocrinol. 2012;10:49.
  • Ahluwalia P, Tewari K, Choudhary P. Studies on the effects of monosodium glutamate (MSG) on oxidative stress in erythrocytes of adult male mice. ToxicolLett. 1996;84:161-5.
  • Seiva FRF, Chuffa LGA, Braga CP, Amorim JPA, Fernandes AAH. Quercetin ameliorates glucose and lipid metabolism and improves antioxidant status in postnatally monosodium glutamate-induced metabolic alterations. Food ChemToxicol. 2012;50:3556-61. da Cunha NV, Pinge-Filho P, Neto OB, Grassiolli S, Martins-Pinge MC. COX-2 inhibition does not reverse the increased sympathetic modulation in MSG obese rats. AutonNeurosci. 2011;165:201–4.
  • El-Kenawy AEM, Osman HEH, Daghestani MH. The effect of vitamin C administration on monosodium glutamate induced liver injury. An experimental study. ExpToxicolPathol. 2013;65:513-21.
  • Kelly G. The interaction of cigarette smoking and antioxidants. part II: alpha – tocopherol. Alternat Med Rev. 2002:7
  • Gagne A, Wei SQ, Fraser WD, Julien P. Absorption, transport, and bioavailability of vitamin e and its role in pregnant Women. J ObstetGynaecol Canada. 2009;31:210-7.
  • Miclea I, Hettgig A, Zahan M, Roman I, Miclea V. The effect of several α-tocopherol concentrations on swine oocyte maturation and embryo culture. Bulletin UASVM AnimSciBiotechnol. 2009;1-2:385-92.
  • Mozes S, Sefcikova L, Lenharde Z, Raeek L. Obesity and changes of alkaline phosphatase activity in the small intestine of 40–80- day old subjects to early postnatal overfeeding of monosodium glutamate. Physiol Res 2004;53:177–86.
  • Alalwani AD. Monosodium glutamate induced testicular lesions in rats (histological study). Middle East FertilSoc J 2013; http://dx/doi/org/10.1016/j.mefs.2013.09.003
  • Diniz YS, Faine LA, Galhardi CM, Rodrigues HG, Ebaid GX, Burneiko RC. Cicogna AC. Novelli ELB. Monosodium glutamate in standart and high-fivber diets: metabolic syndrome and oxidative stress in rats. Nutrition 2005;21:749-55.
  • Eweka AO, Om’Iniabohs F. Histological studies of the effects of monosodium glutamate on the liver of adult Wistar rats. The Internet J Gastroenterol. 2008;6:2.
  • Westwood FR. The female rat reproductive cycle: a practical histological guide to staging. ToxicolPathol. 2008;36:375-84.
  • Olayaki LA, Ajao SM, Jimoh GAA, Aremu IT, Soladoye AO. Effect of vitamin C on malondialdehyde (MDA) in pregnant Nigerian women. J Appl Basic Appl Sci. 2008;4:105-8.
  • McCaskill ML, Kharbanda KK, Tuma DJ, Reynolds J, DeVasure J, Sisson JH, Wyatt TA. Hybrid malondialdehyde and acetaldehyde protein adducts form in the lungs of mice exposed to alcohol and cigarette smoke. Alcohol ClinExp Res. 2011;35:1106
  • Park CH, Kim MY, Sok DE, Kim JH, Lee JH, Kim MR. Butterbur (Petasites japonicas Max.) extract improves lipid profiles and antioxidant activities in monosodium L-glutamate-challenged mice. J Med Food. 2010;13:1216–23.
  • Singh K, Ahluwalia P. Effect of monosodium glutamate on lipid peroxidation and certain antioxidant enzymes in cardiac tissue of alcoholic adult male mice. J Cardiovasc Dis Res. 2012;3:12-8.
  • Pereira CF, de Oliveira CR. Oxidative glutamate toxicity involves mitochondrial dysfuntion and perturbation of intracellular Ca 2+ homeostasis. Neurosci Res. 2000;37:227-36.
  • Franc L, Suescun M, Miranda J, Giovambattista A, Perello M, spinedi E, Calandra R. Testis structure and function in a nongenetichyperadipose rat model at prepubertal and adult ages. Endocrinology. 2006;147:1556–63.
  • Miskowiak B, Kesa B, Limanowski A, Pantayka M, Filipiak B. Long-term effect of neonatal (MSG) treatment on reproductive system of the level rats. Folia Morphol (Warsz). 1999;58:105–13.
  • Kaipia A, Hsueh AJW. Regulation of ovarian follicle atresia. Annual Rev Physiol. 1997;59:349–63.
  • Martimbeau S, Tilly JL. Physiological cell death in endocrine-dependent tissues: an ovarian perspective. ClinEndocrinol. 1997;46:241–54.
  • Motta AB, Estevez A, Franchi A, Perez-Martinez S, Farina M, Ribeiro ML, Lassere A, Gimeno MF. Regulation of lipid peroxidation by nitric oxide and PGF 2 α during luteal regreeion in rats. Reproduction. 2001;121:631-7.
  • YazışmaAdresi / Address for Correspondence: Dr.Wiwin Rohmawati Midwifery Master Study Programme, Faculty of Medicine, Brawijaya University, Jl. Veteran, Malang, East Java, Indonesia Email: asyamwiwin@gmail.com G eliş tarihi/Received on: 04.02.2014
  • Kabul tarihi/Accepted on:03.03.2014

Concomitant exposure to vitamin C and E inhibited monosodium glutamate - induced ovarian toxicity in female rats

Year 2014, Volume: 39 Issue: 3, 517 - 524, 22.07.2014
https://doi.org/10.17826/cutf.61415

Abstract

Purpose: Our study aimed to investigate the combinatorial effect of vitamin C and E on oxidative stress, the number of folicles, and hormonal level in female rats orally exposed to monosodium glutamate (MSG). Materials and Methods:Female, twelve weeks old Wistar rats ingested with of MSG at dose 140 mg/200 gram body weight (bw) with or without combination of vitamin C and E. Twenty five rats were divided into five group (n = 5 each), control group, MSG-treatment group, MSG-treatment + 0.2 mg/g bw vitamin C + 0.04 IU/g bw vitamin E (MSG + CE1), MSG-treatment + 0.4 mg/g bw vitamin C + 0.04 IU/g bw vitamin E (MSG + CE2), and MSG-treatment + 0.6 mg/g bw vitamin C + 0.04 IU/g bw vitamin E (MSG + CE1). Analysis of malondialdehyde (MDA) level was done colorimetrically. Analysis of the number of ovarian follicles was done histopathologically with hematoxylin eosin staining. Analysis of 17&#946;-estradiol and follicle stimulating hormone (FSH) levels were done by ELISA technique. Results: There were significantly (P < 0.05) increased ovarium MDA levels and atresia follicle number in groups exposed to MSG compared to non-exposure group. The level of FSH, 17&#946;-estradiol, the number of primary, secondary, de Graaf follicles were significantly lower in MSG-treatment group compared to control group (P < 0.05). The administration of combined vitamin C and E (second and third dose) significantly (P < 0.05) decreased the MDA levels and the number of atresia follicle compared to the MSG-exposed groups, to reach level in control group (P > 0.05). MSG + CE2 and MSG + CE3 significantly increased FSH level, number of primary follicles, compared to MSG-treatment group (P < 0.05), to reach similar level in control group (P > 0.05). All dose of combined vitamin C and E significantly increased 17&#946;-estradiol level and the number of secondary and de Graaf follicles compared to MSG-treatment group (P < 0.05), to reach significantly higher level compared to control group (P < 0.05). Conclusion: The present data suggest that combination of vitamin C and E as in this study inhibited ovarium toxicity caused by MSG treatment.

References

  • Eweka O. Histological studies of the effects of monosodium glutamate on the kidney of adult Wistar rats. Internet J Health. 2007;6:2.
  • Olney JW. Glutamate induced neuronal neurosis in the infant mouse hypothalamus. J. Neuropath Exp Neurol. 1971;30,75-90.
  • Onakewhor J, Oforofuo I, Singh S. Chronic administration of monosodium glutamate induces oligozoospermia and glycogen accumulation in Wistar rat testes. Afr J Reprod Health. 1998;2:190–7.
  • Oforofuo O, Onakewhor J, Idaewor P. The effect of chronic administration of MSG on the histology of the adult Wistar rat testes. Biosci Res Commun. 1997;9:2.
  • Metawie MAEH, Mouselly M. Antral follicular count as a predictor of ovarian respon in induction of ovulation in patients with anovulatory Infertility. Suez Canal Univ Med J. 2003;6:35-40.
  • Regan KS, Cline JM, Creasy D, Davis B, Foley GL, Lanning L, Latendresse JR, Makris, S, Morton D, Rehm S, Stebbins K. Ovarian follicular counting in the assessment of rodent reproductive toxicity. Toxicol Pathol. 2005;33:409–12.
  • Agarwal A, Mellado AA, Premkumar BJ, Shaman A, Gupta S. The effects of oxidative stress on female reproduction: a review. Reprod Biol Endocrinol. 2012;10:49.
  • Ahluwalia P, Tewari K, Choudhary P. Studies on the effects of monosodium glutamate (MSG) on oxidative stress in erythrocytes of adult male mice. ToxicolLett. 1996;84:161-5.
  • Seiva FRF, Chuffa LGA, Braga CP, Amorim JPA, Fernandes AAH. Quercetin ameliorates glucose and lipid metabolism and improves antioxidant status in postnatally monosodium glutamate-induced metabolic alterations. Food ChemToxicol. 2012;50:3556-61. da Cunha NV, Pinge-Filho P, Neto OB, Grassiolli S, Martins-Pinge MC. COX-2 inhibition does not reverse the increased sympathetic modulation in MSG obese rats. AutonNeurosci. 2011;165:201–4.
  • El-Kenawy AEM, Osman HEH, Daghestani MH. The effect of vitamin C administration on monosodium glutamate induced liver injury. An experimental study. ExpToxicolPathol. 2013;65:513-21.
  • Kelly G. The interaction of cigarette smoking and antioxidants. part II: alpha – tocopherol. Alternat Med Rev. 2002:7
  • Gagne A, Wei SQ, Fraser WD, Julien P. Absorption, transport, and bioavailability of vitamin e and its role in pregnant Women. J ObstetGynaecol Canada. 2009;31:210-7.
  • Miclea I, Hettgig A, Zahan M, Roman I, Miclea V. The effect of several α-tocopherol concentrations on swine oocyte maturation and embryo culture. Bulletin UASVM AnimSciBiotechnol. 2009;1-2:385-92.
  • Mozes S, Sefcikova L, Lenharde Z, Raeek L. Obesity and changes of alkaline phosphatase activity in the small intestine of 40–80- day old subjects to early postnatal overfeeding of monosodium glutamate. Physiol Res 2004;53:177–86.
  • Alalwani AD. Monosodium glutamate induced testicular lesions in rats (histological study). Middle East FertilSoc J 2013; http://dx/doi/org/10.1016/j.mefs.2013.09.003
  • Diniz YS, Faine LA, Galhardi CM, Rodrigues HG, Ebaid GX, Burneiko RC. Cicogna AC. Novelli ELB. Monosodium glutamate in standart and high-fivber diets: metabolic syndrome and oxidative stress in rats. Nutrition 2005;21:749-55.
  • Eweka AO, Om’Iniabohs F. Histological studies of the effects of monosodium glutamate on the liver of adult Wistar rats. The Internet J Gastroenterol. 2008;6:2.
  • Westwood FR. The female rat reproductive cycle: a practical histological guide to staging. ToxicolPathol. 2008;36:375-84.
  • Olayaki LA, Ajao SM, Jimoh GAA, Aremu IT, Soladoye AO. Effect of vitamin C on malondialdehyde (MDA) in pregnant Nigerian women. J Appl Basic Appl Sci. 2008;4:105-8.
  • McCaskill ML, Kharbanda KK, Tuma DJ, Reynolds J, DeVasure J, Sisson JH, Wyatt TA. Hybrid malondialdehyde and acetaldehyde protein adducts form in the lungs of mice exposed to alcohol and cigarette smoke. Alcohol ClinExp Res. 2011;35:1106
  • Park CH, Kim MY, Sok DE, Kim JH, Lee JH, Kim MR. Butterbur (Petasites japonicas Max.) extract improves lipid profiles and antioxidant activities in monosodium L-glutamate-challenged mice. J Med Food. 2010;13:1216–23.
  • Singh K, Ahluwalia P. Effect of monosodium glutamate on lipid peroxidation and certain antioxidant enzymes in cardiac tissue of alcoholic adult male mice. J Cardiovasc Dis Res. 2012;3:12-8.
  • Pereira CF, de Oliveira CR. Oxidative glutamate toxicity involves mitochondrial dysfuntion and perturbation of intracellular Ca 2+ homeostasis. Neurosci Res. 2000;37:227-36.
  • Franc L, Suescun M, Miranda J, Giovambattista A, Perello M, spinedi E, Calandra R. Testis structure and function in a nongenetichyperadipose rat model at prepubertal and adult ages. Endocrinology. 2006;147:1556–63.
  • Miskowiak B, Kesa B, Limanowski A, Pantayka M, Filipiak B. Long-term effect of neonatal (MSG) treatment on reproductive system of the level rats. Folia Morphol (Warsz). 1999;58:105–13.
  • Kaipia A, Hsueh AJW. Regulation of ovarian follicle atresia. Annual Rev Physiol. 1997;59:349–63.
  • Martimbeau S, Tilly JL. Physiological cell death in endocrine-dependent tissues: an ovarian perspective. ClinEndocrinol. 1997;46:241–54.
  • Motta AB, Estevez A, Franchi A, Perez-Martinez S, Farina M, Ribeiro ML, Lassere A, Gimeno MF. Regulation of lipid peroxidation by nitric oxide and PGF 2 α during luteal regreeion in rats. Reproduction. 2001;121:631-7.
  • YazışmaAdresi / Address for Correspondence: Dr.Wiwin Rohmawati Midwifery Master Study Programme, Faculty of Medicine, Brawijaya University, Jl. Veteran, Malang, East Java, Indonesia Email: asyamwiwin@gmail.com G eliş tarihi/Received on: 04.02.2014
  • Kabul tarihi/Accepted on:03.03.2014
There are 30 citations in total.

Details

Primary Language Turkish
Journal Section Research
Authors

Wiwin Rohmawati This is me

Yuni Istiananingsih This is me

Nurdiana Nurdiana This is me

Wisnu Barlianto This is me

Pande Made Dwijayasa This is me

Publication Date July 22, 2014
Published in Issue Year 2014 Volume: 39 Issue: 3

Cite

MLA Rohmawati, Wiwin et al. “Vitamin C-E Ve Monosodyum Glutamata Bağlı Ovaryan Toksisite”. Cukurova Medical Journal, vol. 39, no. 3, 2014, pp. 517-24, doi:10.17826/cutf.61415.