Investigation of cytotoxic effects of Eugenia caryophyllus (Clove)

Year 2018, Volume: 21 Issue: 3, 173 - 177, 17.10.2018

Ceylan Hepokur

https://doi.org/10.7126/cumudj.444426

Cited By: 1

Abstract

Objectives:
Eugenia caryophyllus has been used as a
traditional medicinal herb for thousands of years. It is used as an analgesic for tooth and toothache
with the effect of eugenol substance included in clove. Clove is used at toothpaste and some mouthwash. The
aim of this study was determined of in vitro cytotoxic effects Eugenia caryophyllus on fibroblast
cells.

Materials
and Methods: Cytotoxic
activity of Eugenia caryophyllus was evaluated
using XTT assay.

Results: In the present study, toxicity of
clove that is used as a pain reliever in dentistry has examined on L929 cells. Eugenia
caryophyllus were
incubated at 24 and 48 hours.  The IC₅₀ values of Eugenia caryophyllus (Clove) were found 57,92 ± 0,98 µg/mL in 24 h,
37,97 ± 0,74 µg/mL in 48h.

Conclusions: The acceptable level of cytotoxicity associated with Eugenia caryophyllus requires further evaluation to be used

Keywords

Eugenıa caryophyllus, Cytotoxicity

Eugenıa caryophyllus (Karanfil)’un sitoksisitesinin araştırılması

Abstract

Amaç: Eugenia
caryophyllus binlerce yıldır
geleneksel şifalı bitki olarak kullanılmaktadır. Karanfil içerisindeki öjenol
maddesinin etkisi ile diş ve diş ağrıları için analjezik olarak kullanılır. Karanfil,
diş macunu ve bazı gargaralarda kullanılmaktadır. Bu çalışmanın amacı Eugenia caryophyllus in vitro sitotoksik
etkileri fibroblast hücre hatları üzerinde belirlemiştir.

Gereç ve Yöntem: Eugenia
caryophyllus'un
sitotoksik aktivitesi XTT testi kullanılarak değerlendirildi.

Bulgular: Bu çalışmada diş hekimliğinde ağrı kesici olarak
kullanılan karanfil toksisitesi L929 hücreleri üzerinde incelenmiştir. Eugenia caryophyllus 24 ve 48 saatlerde inkübe edildi. Eugenia
caryophyllus'un
(Karanfil) IC₅₀ değerleri 24 saat de 37,92 ± 0,98 µg / mL, 48 saatte
de 37,97 ± 0,74 µg / mL olarak bulundu.

Sonuç: Eugenia caryophyllus ile ilişkili kabul edilebilir sitotoksisite seviyesi,
kullanılmak üzere daha fazla değerlendirme gerektirir.

Keywords

Eugenıa caryophyllus, Sitotoksisite

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