Proliferation of Mesenchymal Stem Cells in Carbonate Apatite-Chitosan Scaffolds in Bone Tissue Engineering Techniques

Year 2019, Volume: 22 Issue: 4, 412 - 418, 29.12.2019

Aqsa Sjuhada Oki Maretaningtias Dwi Ariani

https://doi.org/10.7126/cumudj.595455

Cited By: 1

Abstract

Objectives: Chitosan (poly-1,4-D-glucosamine) become popular
as bone graft material. It has a high biocompatibility and biodegradability,
non-toxic, bacteriostatic, and has good adsorption properties. It also enhances
the proliferation and differentiation of the osteoblast cell cultures. However,
chitosan also has a weakness for cell adhesion and the lack of sufficient bone
formation capabilities. To improve it, we tried to develop a chitosan scaffolds
that combine with carbonate apatite (CA), which has excellent biocompatibility
properties of the tissues of the human body. 

Purpose:
to make the carbonate apatite-chitosan scaffolds (CA-ChSs) and evaluate CA-ChSs
from the standpoint of cell proliferation using mesenchymal stem cells (MSCs). 

Materials and Methods: Porous chitosan matrix was made by using the
lyophilization technique. ChSs containing chitosan powder was made by the
following procedure. 100, 150, 200, and 400 mg of chitosan powder was dissolved
in 5 ml of 2% acetic acid at room temperature. To make CA-ChSs, ChSs containing
200 mg of chitosan powder was chosen. Ultraviolet radiation was then performed
for 2 hours. CA-ChSs structure was observed by a scanning electron microscope
(SEM). Proliferation of MSCs in CA-ChSs evaluated at days 1, 3, 5 and 7.

Results:This study demonstrated that CA-ChSs containing
200 mg of chitosan powder and 50 mg CA has a three-dimensional structure that
is porous and attachment powder CA in the pores and absorbance values were
increased from the examination day 1 until day 7. 

Conclusion:Based on these results, it is expected CA-ChSs
may be candidates for bone graft material in tissue engineering techniques.

Keywords

chitosan, scaffold, bone regeneration, tissue engineering, mesenchymal stem cell

References

• Debnath, T. Ghosh, S. Potlapuvu, US. Kona, L, Kamaraju, SR. Sarkar, S. Gaddam, S. Chelluri, LK. Proliferation and differentiation potential of human adipose-derived stem cells grown on cytosan hydrogel. Plos One. 2015; 10(3): e0120803.