Adhesion of Candida Albicans and Candida Parapsilosis to Different Restorative Materials

Year 2019, Volume: 22 Issue: 4, 461 - 468, 29.12.2019

Soley Arslan Ayşe Nedret Koç Burhanettin Avcı Hacer Balkaya Nazire Nurdan Çakır

https://doi.org/10.7126/cumudj.644750

Cited By: 1

Abstract

Objective: The aim of this study is to compare the susceptibility
of seven different restorative materials (three conventional composite resins,
two bulk-fill composite resins, one giomer, and one high viscosity glass
ionomer material) to adhere Candida
albicans and Candida parapsilosis.

Material and methods: In this
study, thirty cylindrical specimens of each material were made according to
instructions of the manufacturers. The surface roughness of the specimens was
assessed using a profilometer. Thereafter, the specimens were incubated with a
reference strain of Candida albicans (ATCC
64548) and Candida parapsilosis (ATCC
22019). The proliferated colonies counted as CFU/ml. One-way analysis of
variance (ANOVA) was used to evaluate the surface roughness and the adhesion
value of the materials. Tukey’s post-hoc test was used for subsequent pairwise
comparisons.

Results: There was a statistically significant difference
between the groups in terms of the surface roughness of the materials (p<0.05). The high viscosity glass
ionomer material exhibited significantly higher surface roughness values while X-trafil
(a bulkfill composite resin) had the lowest surface roughness values. Also, there
was a significant difference between Candida
adhesion values of the materials (p<0.05).

Conclusion: There was no significant relationship between surface
roughness and adhesion of Candida
albicans and Candida parapsilosis.
Involvement was seen more for Candida albicans compared to Candida
parapsilosis in all restorative materials.

Keywords

Bulk-fill composite, candida adhesion, restorative material, surface roughness

References

• 1. Loesche WJ. Role of Streptococcus mutans in human dental decay. Microbiol Rev 1986;50(4):353-380.
• 2. Bjorndal L, Larsen T. Changes in the cultivable flora in deep carious lesions following a stepwise excavation procedure. Caries Res 2000;34(6):502-508.
• 3. Takahashi N, Nyvad B. Caries ecology revisited: microbial dynamics and the caries process. Caries Res 2008;42(6):409-418.
• 4. Cura F, Palmieri A, Girardi A, Martinelli M, Scapoli L, Carinci F. Lab-Test((R)) 4: Dental caries and bacteriological analysis. Dent Res J (Isfahan) 2012;9(Suppl 2):S139-141.
• 5. Udayalaxmi J, Shenoy N. Comparison Between Biofilm Production, Phospholipase and Haemolytic Activity of Different Species of Candida Isolated from Dental Caries Lesions in Children. J Clin Diagn Res 2016;10(4):DC21-23.
• 6. Beldüz N, Kamburoğlu A, Yılmaz Y, Tosun I, Beldüz M, Kara C. Evaluation of candida albicans biofilm formation on various dental restorative material surfaces. Niger J Clin Pract 2017;20(3):355-360.
• 7. Negroni M, González MI, Levin B, Cuesta A, Iovanniti C. Candida carriage in the oral mucosa of a student population: adhesiveness of the strains and predisposing factors. Rev Argent Microbiol 2002;34(1):22-28.
• 8. Kadir T, Uygun B, Akyuz S. Prevalence of Candida species in Turkish children: relationship between dietary intake and carriage. Arch Oral Biol 2005;50(1):33-37.
• 9. Elguezabal N, Maza JL, Ponton J. Inhibition of adherence of Candida albicans and Candida dubliniensis to a resin composite restorative dental material by salivary secretory IgA and monoclonal antibodies. Oral Dis 2004;10(2):81-86.
• 10. Pereira-Cenci T, Cury AA, Cenci MS, Rodrigues-Garcia RC. In vitro Candida colonization on acrylic resins and denture liners: influence of surface free energy, roughness, saliva, and adhering bacteria. Int J Prosthodont 2007;20(3):308-310.
• 11. Waltimo T, Tanner J, Vallittu P, Haapasalo M. Adherence of Candida albicans to the surface of polymethylmethacrylate--E glass fiber composite used in dentures. Int J Prosthodont 1999;12(1):83-86.
• 12. Ilie N, Bucuta S, Draenert M. Bulk-fill resin-based composites: an in vitro assessment of their mechanical performance. Oper Dent 2013;38(6):618-625.
• 13. Benetti AR, Havndrup-Pedersen C, Honoré D, Pedersen MK, Pallesen U. Bulk-fill resin composites: polymerization contraction, depth of cure, and gap formation. Oper Dent 2015;40(2):190-200.
• 14. Friedl K, Hiller KA, Friedl KH. Clinical performance of a new glass ionomer based restoration system: a retrospective cohort study. Dent Mater 2011;27(10):1031-1037.
• 15. Kimyai S, Savadi-Oskoee S, Ajami AA, Sadr A, Asdagh S. Effect of three prophylaxis methods on surface roughness of giomer. Med Oral Patol Oral Cir Bucal 2011;16(1):e110-114.
• 16. Muadcheingka T, Tantivitayakul P. Distribution of Candida albicans and non-albicans Candida species in oral candidiasis patients: Correlation between cell surface hydrophobicity and biofilm forming activities. Arch Oral Biol 2015;60(6):894-901.
• 17. Trofa D, Gácser A, Nosanchuk JD. Candida parapsilosis, an emerging fungal pathogen. Clin Microbiol Rev 2008;21(4):606-625.
• 18. Lazarin AA, Machado AL, Zamperini CA, Wady AF, Spolidorio DM, Vergani CE. Effect of experimental photopolymerized coatings on the hydrophobicity of a denture base acrylic resin and on Candida albicans adhesion. Arch Oral Biol 2013;58(1):1-9.
• 19. Maza JL, Elguezabal N, Prado C, Ellacuría J, Soler I, Pontón J. Candida albicans adherence to resin-composite restorative dental material: influence of whole human saliva. Oral Surg Oral Med Oral Pathol Oral Radiol Endod 2002;94(5):589-592.
• 20. Radford DR, Sweet SP, Challacombe SJ, Walter JD. Adherence of Candida albicans to denture-base materials with different surface finishes. J Dent 1998;26(7):577-583.
• 21. Bürgers R, Schneider-Brachert W, Rosentritt M, Handel G, Hahnel S. Candida albicans adhesion to composite resin materials. Clin Oral Investig 2009;13(3):293-299.
• 22. Mayahara M, Kataoka R, Arimoto T, Tamaki Y, Yamaguchi N, Watanabe Y, Yamasaki Y, Miyazaki T. Effects of surface roughness and dimorphism on the adhesion of Candida albicans to the surface of resins: scanning electron microscope analyses of mode and number of adhesions. J Investig Clin Dent 2014;5(4):307-312.
• 23. Kawai K, Takaoka T. Inhibition of bacterial and glucan adherence to various light-cured fluoride-releasing restorative materials. J Dent 2001;29(2):119-122.
• 24. Rawls HR, Zimmerman BF. Fluoride-exchanging resins for caries protection. Caries Res 1983;17(1):32-43.
• 25. Kothavade RJ, Kura MM, Valand AG, Panthaki MH. Candida tropicalis: its prevalence, pathogenicity and increasing resistance to fluconazole. J Med Microbiol 2010;59(Pt 8):873-880.
• 26. van Asbeck EC, Clemons KV, Stevens DA. Candida parapsilosis: a review of its epidemiology, pathogenesis, clinical aspects, typing and antimicrobial susceptibility. Crit Rev Microbiol 2009;35(4):283-309.
• 27. Laffey SF, Butler G. Phenotype switching affects biofilm formation by Candida parapsilosis. Microbiol 2005;151(Pt 4):1073-1081.
• 28. Wingard JR. Importance of Candida species other than C albicans as pathogens in oncology patients. Clin Infect Dis 1995;20(1):115-125.
• 29. Pereira CA, Costa AC, Silva MP, Back-Brito GN, Jorge AO. Candida albicans and virulence factors that increases its pathogenicity. In A. Méndez-Vilas, editor. The Battle Against Microbial Pathogens: Basic Science, Technological Advances and Educational Program. Badajoz, Spain: Formatex 2015:631-636.
• 30. Brunel L, Neugnot V, Landucci L, Boze H, Moulin G, Bigey F, et al. High-level expression of Candida parapsilosis lipase/acyltransferase in Pichia pastoris. J Biotechnol 2004;111(1):41-50.