In vitro Cytogenetic Analysis in Human Cultured Blood Lymphocytes of Newly Synthesızed Two Different Benzoic Acid Derivatives [(4-(3-acetyl-2-methyl-4-phenyl-1H-pyrrol-1-yl) Benzoic Acid) and (4-(3-cinamoyl-2-methyl-4-phenyl-1H-pyrrol-1-yl) Benzoic Acid)]

Year 2022, Volume: 1 Issue: 1, 6 - 20, 30.01.2022

Rumeysa Meşe Hasan Basri İla

Abstract

Globally, benzoic acid and its various derivatives are widely used as antibacterial and antifungal agents, as well as in the hygiene, cosmetic, food, and pharmaceutical industries. This study is planned to reveal the cytotoxic and genotoxic identity of the newly synthesized pyrrole derivative molecules [4-(3-acetyl-2-methyl-4-phenyl-1H-pyrrol-1-yl) Benzoic Acid (D085)] and pyrrole-chalcone hybrid derivative [4-(3-cinamoyl-2-methyl-4-phenyl-1H-pyrrol-1-yl) Benzoic Acid (D085-K)]. Experiments were performed using in vitro chromosome aberration (CA) and micronucleus (MN) tests in human peripheral lymphocytes (HPL). The test substance concentrations used in the study were determined via a preliminary study. Based on the genotoxic and cytotoxic effects of both test compounds, the 24-hour treatment was found to be more effective than the 48-hour treatment. The fact that the induced MN frequency is similar to the CA frequency gives a preliminary idea about the clastogenic potential of the test substances. Compound D085 induced a base change mutation, while D085-K induced a frameshift mutation. Furthermore, in this study, high concentrations of the test compounds were generally found to be cytotoxic according to MI and NDI data as indicators of cytotoxicity.

Keywords

4-(3-acetyl-2-methyl-4-phenyl-1H-pyrrol-1-yl) Benzoic Acid, 4-(3-cinamoyl-2-methyl-4-phenyl-1H-pyrrol-1-yl) Benzoic Acid, Genotoxicity, Chromosome Aberration (CA), Micronucleus (MN), Ames/Salmonella/Microsome Bacterial Reverse Mutation Test

Supporting Institution

Çukurova Üniversitesi

Project Number

FYL-2018-10557

Thanks

This research was funded by Unit of Scientific Research Projects, Cukurova University (CUBAP), Turkey.

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