EVALUATION OF GENISTEIN CONTENT IN CHICKPEA (CICER ARIETINUM L.) AND MUNG BEAN VIGNA RADIATA L.) SPROUTS GERMINATED UNDER DIFFERENT CONDITIONS

Year 2019, Volume: 2 Issue: 1, 1 - 10, 29.07.2019

Jaya Arora , Bhanupriya Kanthalıya Abhishek Joshı

Abstract

Cicer arietinum L. (chickpea)
and Vigna radiata L. (mung bean) are
two important pulses, being continuously used by a large number of populations all over the world. Sprouts of both
pulses are also gaining importance due to the presence
of high level of dietary fibers, vitamin B-Complex, vitamin A, vitamin C,
omega-3 fatty acids and proteins. There are lots of studies done on evaluation
of total isoflavonoids content present in
seeds of various pulses including soybean,
chickpea, lentil etc. Various biochemical parameters of sprouts of both pluses
have also been studied till date but no specific studies on secondary
metabolite content during sprouting have been done. The aim of the present
study was an evaluation of growth and
genistein content from 1^st day to 6^th day of sprouting,
evaluation of different growth conditions (BOD, in vitro, in vivo) for
the same parameters along with the effect
of  prohexadione
on the sprouts of both pluses have been done. Among the three studied genotypes
of chickpea, CSCD-884 genotype was
the best for the high genistein content. However, in case of mung bean, V. RMW 344 genotype contains higher
genistein content. With the change in growth condition, in vitro group give best results in genistein content in mung bean
sprouts (28.32 fold higher) while in case of chickpea sprouts BOD group give
the best result (12.8 fold higher).
Remarkably, lower concentrations of prohexadione-pure treatment enhance the
genistein content in both the chickpea and mung
bean sprouts at BOD condition. A significant increase was also observed
in genistein content in chickpea sprouts while treated with prohexadion-calcium at in vitro condition. This study also reveals that mung beans can
supplement higher levels of genistein contents than chickpeas if sprouted at
controlled culture conditions. This finding could expand the potential for the
development of both pulses sprouts as a functional food by giving stress
treatment and enhancing the total isoflavonoid contents. 

Keywords

Cicer arietinum sprout, Vigna radiata sprout, Elicitation, Prohexadione, Genistein

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