Ethnopharmacological Survey on Phlomis grandiflora: in vivo Antihemorrhoidal Model

Year 2019, Volume: 2 Issue: 1, 40 - 46, 29.07.2019

Ceylan Dönmez , Ufuk Koca Çalışkan

https://doi.org/10.38093/cupmap.596972

Cited By: 1

Abstract

Phlomis grandiflora H.S Thompson
var. grandiflora, belongs to
Lamiaceae family, has been utilized traditionally for the treatment of wound,
inflammation, ulcer, diabetes and hemorrhoid mostly. Though hemorrhoids are so
widespread in the world, studies on the prevention and treatment with natural
pharmaceutical preparations of them are quite limited. People, who suffer from
hemorrhoids, tries variety of self treatment methods such as utilizing animal
products and homemade herbal preparations. Aim of this study is to evaluate the
ethnopharmacological application of P.
grandiflora on antihemorrhoidal activity of the leaf methanolic extract,
which was applied topically (50 % cream) for 7 days onto rats having croton
oil-induced hemorrhoid. Changes in the biochemical parameters such as TNF-α and VEGF showed that the plant extract
is slightly more effective at healing hemorrhoids compared to the reference preparation.
This is the first report revealing that the methanolic leaf extract of P. grandiflora showed topical antihemorrhoidal
activity. When chemical studies are taken into consideration, it has been
concluded that the plant extract containing a significant level of phenolic
compounds, might be a promising source for the investigation of the antihemorrhoidal
effect of medicinal plants, in the means of P.
grandiflora, and related disturbances.

Keywords

Ethnopharmacology, hemorrhoid, Phlomis, medicinal food, traditional medicine

Supporting Institution

Gazi University Scientific Research Projects Unit

Project Number

02/2016-03

Thanks

We would like to thank Gazi University Scientific Research Projects Unit for its project (02/2016-03 code number) fund support. We would like to thank Vet. Salih Salar from Kobay Experimental Animals Laboratory.

References

• Abascal, K., and Yarnell, E., 2005. Botanical treatments for hemorrhoids. Alternative & Complementary Therapies, 11(6), 285-289.
• Aka-Dönmez, C., Boyacioğlu, Ö., Korkusuz, P., and Koca-Çalışkan, U., 2019. The Fifth International Mediterranean Symposium on Medicinal and Aromatic Plants. In: From a Traditional Remedy to Modern therapy; in vivoAntihemorrhoidal Study of Malvasylvestris L., MESMAP, 5, Nevşehir, 260-265.
• Amor, I.L.B., Boubaker, J., Sgaier, M.B., Skandrani, I., Bhouri, W., Neffati, A., Kilani, S., Bouhlel, I., Ghedira, K. and Chekir-Ghedira, L., 2009. Phytochemistry and biological activities of Phlomis species. Journal of Ethnopharmacology, 125(2), 183-202.
• Azeemuddin, M., Viswanatha, G.L., Rafiq, M., Thippeswamy, A.H., Baig, M.R., Kavya, K. J., and Shyam, R., 2014. An improved experimental model of hemorrhoids in rats: evaluation of antihemorrhoidal activity of an herbal formulation. ISRN Pharmacology, 2014, 1-7.
• Bates, D.O., 2010. Vascular endothelial growth factors and vascular permeability. Cardiovascular Research, 87(2), 262-271.
• Çelik, S., Göktürk, R. S., Flamini, G., Cioni, P. L., and Morelli, I., 2005. Essential oils of Phlomis leucophracta, Phlomis chimerae and Phlomis grandiflora var. grandiflora from Turkey. Biochemical Systematics and Ecology, 33(6), 617-623.
• Demirci, F., Guven, K., Demirci, B., Dadandi, M.Y., and Baser, K.H.C., 2008. Antibacterial activity of two Phlomis essential oils against food pathogens. Food Control, 19(12), 1159-1164.
• Dey, Y.N., Wanjari, M.M., Kumar, D., Lomash, V., andJadhav, A.D., 2016. Curativeeffect of Amorphophallus paeoniifolius tuber on experimental hemorrhoids in rats. Journal of Ethnopharmacology, 192, 183-191.
• Dönmez, C., 2019. Pharmacognosic researches on Solanum melongena L. used against hemorrhoid as folk remedy [Halk arasında hemoroide karşı kullanılan Solanum melongena L. bitkisi üzerinde farmakognozik araştırmalar], Gazi University, Department of Pharmacognosy, Ankara, 65-85.
• Gürbüz, İ., Üstün, O., Yesilada, E., Sezik, E., and Kutsal, O., 2003. Anti-ulcerogenic activity of some plants used as folk remedy in Turkey. Journal of Ethnopharmacology, 88(1), 93-97.
• Koca-Çalışkan, U., Aka, C., Anlaş, F.C., Üstün-Alkan, F., and Üstüner, O., 2017a. Mediterranean Symposium on Medicinal and Aromatic Plants. In: The Antioxidant and Proliferative Activities of Different Extracts from Some Medicinal Plants on Mouse Fibroblast Cells. MESMAP, 3, Kyrenia, 235.
• Koca-Çalışkan, U., Aka, C., and Oz, M.G., 2017b. Plants used in Anatolian traditional medicine for the treatment of hemorrhoid. Records of Natural Products, 11(3), 235-250.
• Nishiki, K., Nishinaga, K., Kudoh, D., and Iwai, K., 1988. Croton oil-induced hemorrhoid model in rat: comparison of anti-inflammatory activity of diflucortolone valerate with other glucocorticoids. Nihonyakurigakuzasshi. Folia Pharmacologica Japonica, 92(4), 215-225.
• Şenol, F.S., Günbatan, T., Öztürk, N., Gürbüz, İ., and Orhan, İ.E., 2018. Phenolic acid profile and in vitro neurobiological effect of Phlomis grandiflora HS Thompson var. grandiflora. Journal of the Chemical Society of Pakistan, 40(1), 129-138.
• Singleton, V.L., and Rossi, J.A., 1965. Colorimetry of total phenolics with phosphomolybdic-phosphotungstic acid reagents. American Journal of Enology and Viticulture, 16, 144-158.
• Takeda, Y., Kinugawa, M., Masuda, T., Honda, G., Otsuka, H., Sezik, E., and Yesilada, E., 1999. Phlomisethanoside, a phenylethanoid glycoside from Phlomis grandiflora var. grandiflora. Phytochemistry, 51(2), 323-325.
• Varut, L., 2012. Hemorrhoids: from basic pathophysiology to clinical management. World Journal of Gastroenterology, 18(17), 2009-2017.
• Woisky, R.G., and Salatino, A., 1998. Analysis of propolis: some parameters and procedures for chemical quality control. Journal of Apicultural Research, 37, 99-105.