Year 2019, Volume 2 , Issue 2, Pages 93 - 99 2019-12-26

Identification and Authentication of Microbes Causing Urinary Tract Infection and Detection of Antibacterial Activity for Methanolic Extract of Senna alexanderina against these Pathogenic Bacteria in Khartoum State, Sudan

Abubker O. Babiker [1] , Areeg A. Abdelwakeel [2] , Ibrahim F. Ahmed [3] , Ahmed Elshikh [4]


The aim of this study is to isolate and identify the microbes causing urinary tract infection and antibacterial of those microbes by used methanolic extract of plant and antibiotics. One hundred samples were collected for both genders in Khartoum State. From sixty-three out of one hundred samples obtained on different types of microbes are Staphylococcus   aureus   (33.3%), Enterococcus fecales (9.5%), Escherichea coli (19%), Klebsiella pneumonae (7.9%), Protus mirabilis, Pseudomonas aeuroginosa (9.5%), Citrobacter  ssp (4.7%), Candida albicans (7.9%). The antibacterial results against isolated microorganisms using methanolic extract of Senna alexanderina showed resistance to these microbes except S. aureus was sensitive; also, most microbes were sensitive to antibiotics.

urinary tract infection, methanolic extract, Senna alexanderina, Pathogenic Bacteria, Microbes
  • References 1. Abeysinghe P.D. (2010). Antibacterial activity of some medicinal mangroves against antibiotic resistant pathogenic bacteria. Indian Journal of Pharmaceutical Science, 72, 167-72
  • 2. Dole C. (2004). In the shadows of medicinal and modernity medicinal integration and secular histories of religious healing in turkey journal culture. Medicinal Psychiatry, 28(3),255-80.
  • 3. Gezici S. and Sekeroglu N. (2019). Current perspectives in the application of medicinal plants against cancer: novel therapeutic agents. Anti-Cancer Agents in Medicinal Chemistry (Formerly Current Medicinal Chemistry-Anti-Cancer Agents), 19(1), 101-111.
  • 4. Gomez MR, Cerutti S, Sombra LL, Silva MF and Martı´nez LD. (2007). Determination of heavy metals for the quality control in Argentinian herbal medicines by ETAAS and ICP-OES. Food Chem Toxicol 45:1060–1064.
  • 5. Kanwar K., Pandey R., Gezici S., Azmi W. (2019). Enzymes as competent tool for efficient management of pathogen’s biofilms. Annals of Phytomedicine, 8(1), 70-81.
  • 6. Kavanagh F. (1972). Analytical Microbiology, Vol II, Academic Press, New York and London, pp. 11.
  • 7. Miles A. A. and Misra S. S., (1938). Estimation of bacterial power of blood, J Hygiene (Lond) 38: 732-49.
  • 8. Saranraj P. and Sivasakthivelan P. (2012). Screening of Antibacterial Activity of the Medicinal Plant Phyllanthus amarus Against Urinary Tract Infection Causing Bacterial Pathogens, Applied Journal of Hygiene, 1(3), 19-24.
  • 9. Selim S. A., El Alfy S. M., Abdel Aziz M. H., Mashait M. and Warrad M. F. (2013). Antibacterial activity of selected Egyptian ethnomedicinal plants, Malaysian Journal of Microbiology, 9(1), pp. 111-115.
  • 10. Sharma A., Verma R. and Ramteke P. (2009). Antibacterial Activity of Some Medicinal Plants Used by Tribals Against Uti Causing Pathogens, World Applied Sciences Journal, 7(3), 332-339.
  • 11. Shida W., Tateishi H., Tahara, Y. Fujita, M. Husham Majeed Alsaadi D., Watanabe M., Koga R., Radwan M.O., Ciftci H.I., Gezici S., Kurauchi Y., Katsuki H., Otsuka M., Sugimura K., Wada M., Sekeroglu N., Watanabe T. (2019). Antileukemic Activity of Twig Components of Caucasian Beech in Turkey. Molecules, 24(21), 3850.
  • 12. Viswanathan S. and Nallamuthu T. (2012). Phytochemical screening and antimicrobial activity of leaf extracts of Senna alexandrina Mill. against human pathogens, International Journal of Current Science, 2, 51-56.
  • 13. World Health Organization (WHO), (1988). The international pharmacopoeia, 3rd ed. Vol. 3. Quality specifications. Geneva.
Primary Language en
Subjects Science
Published Date December
Journal Section Articles
Authors

Author: Abubker O. Babiker
Institution: UNIVERSITY OF HEALTH SCIENCES, SUDAN NYALA VOCATIONAL SCHOOL OF HEALTH SCIENCES
Country: Sudan


Author: Areeg A. Abdelwakeel
Institution: UNIVERSITY OF HEALTH SCIENCES, HARTUM GRADUATE SCHOOL OF HEALTH SCIENCES (SUDAN)
Country: Sudan


Author: Ibrahim F. Ahmed
Institution: UNIVERSITY OF HEALTH SCIENCES, HARTUM GRADUATE SCHOOL OF HEALTH SCIENCES (SUDAN)
Country: Sudan


Orcid: 0000-0002-0682-0312
Author: Ahmed Elshikh
Institution: International University of Africa
Country: Sudan


Thanks We are grateful to Department of Microbiology, Faculty of Pure and Applied Sciences, International University of Africa, Khartoum, Sudan.
Dates

Publication Date : December 26, 2019

APA Babiker, A , Abdelwakeel, A , Ahmed, I , Elshikh, A . (2019). Identification and Authentication of Microbes Causing Urinary Tract Infection and Detection of Antibacterial Activity for Methanolic Extract of Senna alexanderina against these Pathogenic Bacteria in Khartoum State, Sudan. Current Perspectives on Medicinal and Aromatic Plants (CUPMAP) , 2 (2) , 93-99 . Retrieved from https://dergipark.org.tr/en/pub/cupmap/issue/51022/650509