Spondias pinnata (L.f.) Kurz.(Anacardiaceae) is widely used as food and for medicinal properties. This study aims to disclose the free radical scavenging potential, total phenolic and flavonoid contents and phytochemical constituents of 70% methanol extract of unripe fruits of S. pinnata collected from Kaski district, Nepal. The free radical scavenging activity was evaluated by 1,1-diphenyl-2-picrylhydrazyl (DPPH) assay method. The total phenolic content (TPC) and total flavonoid content (TFC) were estimated by using Folin-Ciocalteu’s phenol reagent and aluminium chloride methods, respectively. S. pinnata fruits extract showed potent free radical scavenging activity with IC50 value 2.75±0.23 μg/ml. TPC and TFC values were found to be 229.24±0.46 mg GAE/g and 192.58±3.81 mg QE/g, respectively. Detailed chemical isolation of the extract afforded caffeic acid methyl ester (1) and rhamnetin 3-O-sophoroside (2). In conclusion, S. pinnata fruits were found to be rich source of phenolic and flavonoid compounds and possessed strong free radical scavenging property. However, further study is needed to explore its potential health benefits and bioassay guided chemical analysis should be performed to isolate and identify the bioactive compounds.
Spondias pinnata, DPPH, total phenolic content, total flavonoid content, isolation