Year 2020, Volume 3 , Issue 1, Pages 54 - 60 2020-06-27

Free Radical Scavenging Activity and Chemical Constituents of the Unripe Fruits of Spondias pinnata (L.f.) Kurz. from Nepal

Kusum SAI [1] , Hari Prasad DEVKOTA [2] , Rashmi THAPA [3] , Prakash POUDEL [4] , Khem Raj JOSHI [5]

Spondias pinnata (L.f.) Kurz.(Anacardiaceae) is widely used as food and for medicinal properties. This study aims to disclose the free radical scavenging potential, total phenolic and flavonoid contents and phytochemical constituents of 70% methanol extract of unripe fruits of S. pinnata collected from Kaski district, Nepal. The free radical scavenging activity was evaluated by 1,1-diphenyl-2-picrylhydrazyl (DPPH) assay method. The total phenolic content (TPC) and total flavonoid content (TFC) were estimated by using Folin-Ciocalteu’s phenol reagent and aluminium chloride methods, respectively. S. pinnata fruits extract showed potent free radical scavenging activity with IC50 value 2.75±0.23 μg/ml. TPC and TFC values were found to be 229.24±0.46 mg GAE/g and 192.58±3.81 mg QE/g, respectively. Detailed chemical isolation of the extract afforded caffeic acid methyl ester (1) and rhamnetin 3-O-sophoroside (2). In conclusion, S. pinnata fruits were found to be rich source of phenolic and flavonoid compounds and possessed strong free radical scavenging property. However, further study is needed to explore its potential health benefits and bioassay guided chemical analysis should be performed to isolate and identify the bioactive compounds.
Spondias pinnata, DPPH, total phenolic content, total flavonoid content, isolation
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Primary Language en
Subjects Chemistry, Multidisciplinary
Published Date June
Journal Section Articles

Orcid: 0000-0000-0000-0000
Author: Kusum SAI
Institution: Pokhara University
Country: Nepal

Author: Hari Prasad DEVKOTA (Primary Author)
Institution: Kumamoto University
Country: Japan

Orcid: 0000-0000-0000-0000
Author: Rashmi THAPA
Institution: Pokhara University
Country: Nepal

Orcid: 0000-0000-0000-0000
Author: Prakash POUDEL
Institution: Purbanchal University
Country: Nepal

Orcid: 0000-0000-0000-0000
Author: Khem Raj JOSHI
Institution: Pokhara University
Country: Nepal

Thanks Authors are grateful to School of Health and Allied Sciences, Faculty of Health Sciences, Pokhara University for providing laboratory facilities and to Dr. Radheshyam Kayastha, former Professor, Tribhuvan University, Nepal for plant specimen identification.

Publication Date : June 27, 2020

APA Saı, K , Devkota, H , Thapa, R , Poudel, P , Joshı, K . (2020). Free Radical Scavenging Activity and Chemical Constituents of the Unripe Fruits of Spondias pinnata (L.f.) Kurz. from Nepal . Current Perspectives on Medicinal and Aromatic Plants (CUPMAP) , 3 (1) , 54-60 . DOI: 10.38093/cupmap.730458