GC-MS/MS and 1H-NMR analysis of endemic Campanula baskilensis Behçet leaf fractions

Year 2024, Volume: 7 Issue: 1, 48 - 57, 06.07.2024

Sarmad Marah , Tevfik Ozen , İbrahim Demirtas , Yaşar İpek , Fatih Gül , Lütfi Behçet

https://doi.org/10.38093/cupmap.1477900

Abstract

In this research, 1H-NMR and CG-MS/MS spectrophotometric techniques were used to elucidate the chemical profile of leaf fractions of Campanula baskilensis Behçet. A column chromatography system was used for the fractionation of the crude extract. Fifteen separate fractions were obtained throughout the fractionation procedure. Thirty-nine molecules were identified using both spectrometry techniques. Thirty-one compounds of these molecules were identified, and eight compounds were listed as unknown. For lupeyl acetate, it was determined as 77.80% in Fr6-2, 63.21% in Fr3-3, 43.81% in Fr4-3, and for 1-octadecene, it was determined as 36.23% in Fr3-2 and 32.14% in Fr4-1. Additionally, levels of 34.88% for hexadecanoic acid and 34.05% for borneol were recorded at Fr8. It is also noteworthy that, as an unknown molecule, Fr3-4 is formed at a rate of 97.28%. Molecular structures such as hexadecanoic acid (methyl palmitate), benzoic acid, eicosanoic acid, limonene, borneol, and hentriacontanol were supported by 1H-NMR analysis.
Understanding the chemical constituents of C. baskilensis plant will provide opportunities to suggest broader research in the future. The identified molecules can be the subject of isolation and further original models for various in-vitro and in-silico bioassays or effective drug development and application.

Keywords

C. baskilensis, GC-MS/MS, ¹H-NMR, Fractionation

Project Number

PYO.FEN.1904.20.003

Thanks

Ondokuz Mayıs Üniversitesi, BAPKOB

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