Farklı Seyreltik Sodyum Hidroksit Ön İşlem Metotlarının Kargı Kamışı (Arundo donax L.) Bitkisinden Fermente Olabilir Şeker ve Etanol Üretimine Etkileri

Year 2021, Volume: 36 Issue: 1, 81 - 92, 30.06.2021

Recep İrfan Nazlı

Abstract

Bu çalışma 5 farklı seyreltik sodyum hidroksit (NAOH) ön işlem metodunun (%1.0, 1.5, 2.0, 2.5 ve 3.0) kargı kamışı bitkisinden biyoetanol üretimine etkilerini araştırmak amacıyla yürütülmüştür. Ön işlem metotları otoklav koşullarında 121 °C’de ve 30 dakika süreyle yürütülmüştür. Araştırmada en yüksek indirgen şeker verimi %2.5 NAOH ön işlem metodundan elde edilmesine karşın, yüksek katı veriminden dolayı en yüksek teoriksel etanol verimi %1.0 NAOH ön işlem metodundan elde edilmiştir. Bu sonuçlar, araştırmada test edilen ön işlem metotları içerisinde % 1.0 NAOH ön işlem metodunun kargı kamışı bitkisinden sürdürülebilir bir biyoetanol üretimi gerçekleştirmek için öncelikli olarak tercih edilebileceğini göstermektedir.

Keywords

Sodyum hidroksit, ön işlem, kargı kamışı, etanol, şeker

References

• Aditiya, H.B., Mahlia, T.M.I., Chong, W.T., Nur, H., Sebayang, A.H. (2016) Second generation bioethanol production: A critical review. Renewable and sustainable energy reviews 66:631-653.
• Aita, G.A., Salvi, D.A., Walker, M.S. (2011) Enzyme hydrolysis and ethanol fermentation of dilute ammonia pretreated energy cane. Bioresource Technology 102:4444-4448.
• Barcelos, C.A., Maeda, R.N., Betancur, G.J.V., Pereira, N. (2013) The essentialness of delignification on enzymatic hydrolysis of sugar cane bagasse cellulignin for second generation ethanol production. Waste and Biomass Valorization 4:341-346.
• Boose, A.B., Holt, J.S. (1999) Environmental effects on asexual reproduction in Arundo donax. Weed Research, 39:117–27.
• Cao, W., Sun, C., Liu, R., Yin, R., Wu, X. (2012) Comparison of the effects of five pretreatment methods on enhancing the enzymatic digestibility and ethanol production from sweet sorghum bagasse. Bioresource Technology 111:215-221.
• Chen, B.Y., Chen, S.W., Wang, H.T. (2012) Use of different alkaline pretreatments and enzyme models to improve low-cost cellulosic biomass conversion. Biomass and Bioenergy 39:182-191.
• Chen, M., Xia, L., Xue, P. (2007) Enzymatic hydrolysis of corncob and ethanol production from cellulosic hydrolysate. International Biodeterioration & Biodegradation 59:85-89.
• Cheng, Y.S., Zheng, Y., Yu, C.W., Dooley, T.M., Jenkins, B.M., Vander Gheynst, J.S. (2010) Evaluation of high solids alkaline pretreatment of rice straw. Applied biochemistry and biotechnology 162:1768-1784.
• Choudhary, R., Umagiliyage, A.L., Liang, Y., Siddaramu, T., Haddock, J., Markevicius, G. (2012) Microwave pretreatment for enzymatic saccharification of sweet sorghum bagasse. Biomass and Bioenergy 39:218-226.
• Eliana, C., Jorge, R., Juan, P., Luis, R. (2014) Effects of the pretreatment method on enzymatic hydrolysis and ethanol fermentability of the cellulosic fraction from elephant grass. Fuel 118: 41-47.
• Haq, F., Ali, H., Shuaib, M., Badshah, M., Hassan, S.W., Munis, M.F.H., Chaudhary, H.J. (2016) Recent progress in bioethanol production from lignocellulosic materials: A review. International Journal of Green Energy 13:1413-1441.
• Haque, M.A., Barman, D.N., Kang, T.H., Kim, M.K., Kim, J., Kim, H., Yun, H.D. (2013) Effect of dilute alkali pretreatment on structural features and enhanced enzymatic hydrolysis of Miscanthus sinensis at boiling temperature with low residence time. Biosystems engineering 114:294-305.
• Hendricks, A.T.W., Zeeman, G. (2009) Pretreatments to enhance the digestibility of lignocellulosic biomass. Bioresource Technology 100:10-18.
• Jiang, D., Ge, X., Zhang, Q., Li, Y. (2016) Comparison of liquid hot water and alkaline pretreatments of giant reed for improved enzymatic digestibility and biogas energy production. Bioresource technology 216:60-68.
• Jung, W., Savithri, D., Sharma-Shivappa, R., Kolar, P. (2020) Effect of sodium hydroxide pretreatment on lignin monomeric components of Miscanthus× giganteus and enzymatic hydrolysis. Waste and Biomass Valorization, 11:5891-5900.
• Kärcher, M.A., Iqbal, Y., Lewandowski, I., Senn, T. (2015) Comparing the performance of Miscanthus x giganteus and wheat straw biomass in sulfuric acid based pretreatment. Bioresource technology, 180, 360-364.
• Kaur, K., Phutela, U.G. (2016) Enhancement of paddy straw digestibility and biogas production by sodium hydroxide-microwave pretreatment. Renewable Energy 92:178-184.
• Keshav, P.K., Banoth, C., Anthappagudem, A., Linga, V.R., Bhukya, B. (2018) Sequential acid and enzymatic saccharification of steam exploded cotton stalk and subsequent ethanol production using Scheffersomyces stipitis NCIM 3498. Industrial Crops and Products 125:462-467.
• Khullar, E., Dien, B.S., Rausch, K.D., Tumbleson, M.E., Singh, V. (2013) Effect of particle size on enzymatic hydrolysis of pretreated Miscanthus. Industrial crops and products 44:11-17.
• Kuşvuran, A., Kuşvuran, Ş., Nazlı, R. İ., Tansı, V. (2019) Kargı Kamışı (Arundo donax L.)’nda Tuz Stresinin Morfolojik ve Fizyolojik Özelliklere Etkisi. Yüzüncü Yıl Üniversitesi Tarım Bilimleri Dergisi, 29:233-241.
• Láinez, M., Ruiz, H.A., Castro-Luna, A.A., Martínez-Hernández, S. (2018) Release of simple sugars from lignocellulosic biomass of Agave salmiana leaves subject to sequential pretreatment and enzymatic saccharification. Biomass and bioenergy 118:133-140.
• Lemons e Silva, C.F., Artigas Schirmer, M., Nobuyuki Maeda, R., Araújo Barcelos, C., Pereira, N., (2015) Potential of giant reed (Arundo donax L.) for second generation ethanol production. Electronic Journal of Biotechnology 18:10-15.
• Lemões, J.S., e Silva, C.F.L., Avila, S.P.F., Montero, C.R.S., e Silva, S.D.D.A., Samios, D., Peralba, M.D.C.R. (2018) Chemical pretreatment of Arundo donax L. for second-generation ethanol production. Electronic Journal of Biotechnology 31:67-74.
• Menezes, E.G., do Carmo, J.R., Alves, J.G.L., Menezes, A.G., Guimarães, I.C., Queiroz, F., Pimenta, C.J. (2014) Optimization of alkaline pretreatment of coffee pulp for production of bioethanol. Biotechnology progress 30:451-462.
• Michalska, K., Bizukojć, M., Ledakowicz, S. (2015) Pretreatment of energy crops with sodium hydroxide and cellulolytic enzymes to increase biogas production. Biomass and Bioenergy 80: 213-221.
• Miller, G.L., 1959. Use of dinitrosalicylic acid reagent for determination of reducing sugar. Anal. Chem. 31:426–428.
• Nazlı, R. İ. (2017) Akdeniz iklim koşullarında bazı çok yıllık buğdaygillerin (miskantus, dallı darı, kargı kamışı, yumrulu yem kanyaşı) enerji bitkisi olarak kullanım olanakları. Doktora Tezi. Çukurova Üniversitesi.
• Nazli, R.I., Tansi, V., Öztürk, H.H., Kusvuran, A. (2018) Miscanthus, switchgrass, giant reed, and bulbous canary grass as potential bioenergy crops in a semi-arid Mediterranean environment. Industrial Crops and Products 125:9-23.
• Nazli, R. İ. (2020) Evaluation of different sweet sorghum cultivars for bioethanol yield potential and bagasse combustion characteristics in a semiarid Mediterranean environment. Biomass and Bioenergy 139:105624.
• Niemi, P., Pihlajaniemi, V., Rinne, M., Siika-aho, M. (2017) Production of sugars from grass silage after steam explosion or soaking in aqueous ammonia. Industrial Crops and Products 98: 93-99.
• Nishimura, H., Kamiya, A., Nagata, T., Katahira, M., Watanabe, T. (2018) Direct evidence for α ether linkage between lignin and carbohydrates in wood cell walls. Scientific reports 8:1-11.
• Pandey, A., Soccol, C.R., Nigam, P., Soccol, V.T. (2000) Biotechnological potential of agro-industrial residues. I: sugarcane bagasse. Bioresour. Technol 74:69–80.
• Pesce, G.R., Fernandes, M. C., Mauromicale, G. (2020) Globe artichoke crop residues and their potential for bioethanol production by dilute acid hydrolysis. Biomass and Bioenergy 134:105471.
• Phitsuwan, P., Sakka, K., Ratanakhanokchai, K. (2016) Structural changes and enzymatic response of Napier grass (Pennisetum purpureum) stem induced by alkaline pretreatment. Bioresource technology 218:247-256.
• Rossa, B., Tuaers, A.V., Naidoo, G., Von Willert, D.J. (1998) Arundo donax L. (Poaceae) — a C3 species with unusually high photosynthetic capacity. Botanica Acta, 111:216–221.
• Qing, Q., Wyman, C.E. (2011) Supplementation with xylanase and β-xylosidase to reduce xylo-oligomer and xylan inhibition of enzymatic hydrolysis of cellulose and pretreated corn stover. Biotechnology for biofuels 4:1-12.
• Santos, C.C., de Souza, W., Sant’Anna, C., Brienzo, M. (2018) Elephant grass leaves have lower recalcitrance to acid pretreatment than stems, with higher potential for ethanol production. Industrial Crops and Products 111:193-200.
• Si, S., Chen, Y., Fan, C., Hu, H., Li, Y., Huang, J., Liao, H., Hao, B., Li, Q., Peng, L., Tu, Y., 2015. Lignin extraction distinctively enhances biomass enzymatic saccharification in hemicelluloses-rich Miscanthus species under various alkali and acid pretreatments. Bioresource technology 183:248-254.
• Umagiliyage, A.L., Choudhary, R., Liang, Y., Haddock, J., Watson, D.G. (2015) Laboratory scale optimization of alkali pretreatment for improving enzymatic hydrolysis of sweet sorghum bagasse. Industrial Crops and Products 74:977-986.
• Xu, J., Cheng, J.J., Sharma-Shivappa, R.R., Burns, J.C. (2010) Sodium hydroxide pretreatment of switchgrass for ethanol production. Energy & Fuels 24:2113-2119.
• Xu, J., Cheng, J.J. (2011) Pretreatment of switchgrass for sugar production with the combination of sodium hydroxide and lime. Bioresource technology 102:3861-3868.
• Wang, Z., Keshwani, D.R., Redding, A.P., Cheng, J.J. (2010) Sodium hydroxide pretreatment and enzymatic hydrolysis of coastal Bermuda grass. Bioresource technology, 101:3583-3585.
• Van Soest, P.J. (1963) Use of detergents in the analysis of fibrous feeds. 2. A rapid method for the determination of fiber and lignin. Journal of the Association of Official Agricultural Chemists 46:829–835.
• Yan, X., Cheng, J.R., Wang, Y.T., Zhu, M.J. (2020) Enhanced lignin removal and enzymolysis efficiency of grass waste by hydrogen peroxide synergized dilute alkali pretreatment. Bioresource technology 301:122756.
• Zhang, M., Wang, F., Su, R., Qi, W., He, Z. (2010) Ethanol production from high dry matter corncob using fed-batch simultaneous saccharification and fermentation after combined pretreatment. Bioresource Technology 101:4959-4964.
• Zhu, S., Wu, Y., Yu, Z., Liao, J., Zhang, Y. (2005) Pretreatment by microwave/alkali of rice straw and its enzymic hydrolysis. Process Biochem. 40:3082–3086.