Molecular fingerprinting of Botrytis cinerea population structure from different hosts

Year 2018, Volume: 35 Issue: 2, 121 - 134, 13.12.2018

İlknur Polat , Görkem Sülü Aytül Kitapcı , Emine Gümrükçü Ömür Baysal

https://doi.org/10.16882/derim.2018.410051

Cited By: 1

Abstract

Botrytis cinerea
(teleomorph: Botryotinia fuckeliana)
causes gray mold disease on vegetable crops in greenhouses. Profound knowledge
on pathogen diversity is necessary for efficiently disease management. In this
study, forty-two B. cinerea isolates
collected from 36 different greenhouses in Antalya province of Turkey were
investigated. Twelve SRAP (sequence-related amplified polymorphism) and 18 ISSR
(inter simple sequence repeat) primers producing high polymorphic fragments
were used to genetic diversity of B.
cinerea isolates infecting dill, basil, lettuce, bean, cucumber, tomato,
pepper and eggplant. The unweighted pair-group method with arithmetic average
analysis (UPGMA) was used to evaluate of combined ISSR and SRAP data showing a
similarity range 0.15-0.90 among the isolates. Cophenetic correlation of the
tree was high level (r=0.93). Interestingly, cluster analysis showed a
divergent group consisting of lettuce isolates which were genetically different
from the other isolates. On the other hand, transposable elements (Flipper and Boty) were detected among
isolates from all the hosts. Isolates containing only the Fliper element were detected. The results showed that genetically
characterized B. cinerea populations
by a high level of genetic diversity were associated with genotype flow and the
evolutionary potential of B. cinerea.
In further studies, the newly tested molecular markers are useful and can be
suggested for analyzing of genetic diversity and population structure of this
pathogen on different hosts.

Keywords

ISSR, SRAP, ISSR, SRAP, Gray mold, Genetic diversity, Host differentiation, ISSR, SRAP, Transposable elements

Farklı konukçulardan elde edilen Botrytis cinerea populasyon yapısının moleküler tanılanması

Abstract

Botrytis cinerea (teleomorph:
Botryotinia fuckeliana) örtüaltı
sebze yetiştiriciliğinde kurşuni küf hastalığı etmenidir. Patojende oluşan
farklılıkların bilinmesi hastalıkla mücadelenin etkinliğini arttırmaktadır.
Çalışmada, Türkiye’nin Antalya ilinde yer alan 36 farklı seradan 42 adet izolat
kullanılmıştır. On iki SRAP (sequence-related amplified polymorphism) primer
kombinasyonu ve 18 ISSR (inter simple sequence repeat) primeri dereotu,
fesleğen, marul, fasulye, hıyar, domates, biber ve patlıcandan elde edilen B. cinerea izolatlarının genetik
farklılıklarının belirlenmesinde oldukça yüksek polimorfizm sağlamışlardır.
ISSR ve SRAP markırlardan elde edilen sonuçlar UPGMA (The unweighted pair-group
method with arithmetic average analysis) analizine göre izolatlar arasında 0.15-0.90
oranında değişen benzerlik elde edilmiştir. Ayrıca, cophenetic correlation
değeri r=0.93 ile oldukça yüksek bulunmuştur. Cluster analizi sonuçları
değerlendirildiğinde marul izolatları diğer izolatlara göre oldukça uzak
kümelenmiştir. Ayrıca, tüm izolatlar için tranpozabl elementler (Flipper ve Boty) araştırılmış ve sadece Flipper
element tespit edilmiştir. Elde edilen genetik karakterizasyon sonuçlarına
göre, B. cinerea populasyonunda
oldukça yüksek seviyede genetik farklılıklar bulunmuştur. Bu durum, B.
cinerea’nın evrimselleşme
potensiyeli ve gen akışlarından kaynaklanabilir. Farklı konukçulardan elde
edilen bu patojenin genetik farklılıklarının belirlenmesinde kullanılan
moleküler markırlar, ileride yapılacak çalışmalara da ışık tutmaktadır.

Keywords

ISSR, SRAP, ISSR, SRAP, Kurşuni küf, Genetik farklılık, Konukçu farklılığı, ISSR, SRAP, Transpoazabl elementler

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