Automation, Evaluation The Laboratory Performance And Clinical Usefullness Of A Low Cost Fructosamine Assay

Year 2019, Volume: 33 Issue: 1, 9 - 17, 26.04.2019

Tuncay Küme , Canan Çoker , Abdurrahman Çömlekçi Fırat Bayraktar Funda Doğruak Banu Önvural

https://doi.org/10.5505/deutfd.2019.72324

Abstract

INTRODUCTION: The aim of this study was to optimise and automate a low cost fructosamine assay and then to investigate the relationship between serum fructosamine levels and long-term complications. 
METHODS: Serum fructosamine and HbA1c were measured in non-diabetic controls (n = 146) with a mean age of 60 ± 20 and diabetic subjects (n = 353) with a mean age of 61 ± 11. The optimization of fructosamine analysis by nitrobluetetrazolium method requires the optimal solubility of diformazan and mathematical correction of uric acid interferance. The within-day imprecision and between-day imprecision of the automated method were 0.7% (n=10) and 2.5% (n=10) respectively. 
RESULTS: Mean fructosamine concentrations were significantly increased in DM (342 ± 74 μmol/L) compared with control group (270 ± 47 μmol/L) (p <0.01). This cross-sectional study suggested that fructosamine concentration was not related to long-term complications of DM (p >0.05). However, since it is highly correlated with HbA1c (r = 0.817, p <0.01).
DISCUSSION AND CONCLUSION: Fructosamine test may be a useful laboratory parameter for monitoring glycemia also being an inexpensive, appropriate for automation and relatively simple test. Thus prospective studies are necessary to determine the relationship between fructosamine levels and long-term complications.

Keywords

Type 2 diabetes mellitus, diabetic complications, nonenzymatic glycation, glycemic control, glycated serum proteins, fructosamine

Düşük Maliyetli Bir Fruktozamin Testinin Otomasyonu, Laboratuvar Performansı Ve Klinik Yararlılığının Değerlendirilmesi

Abstract

GİRİŞ ve AMAÇ: Bu çalışmanın amacı düşük maliyetli bir fruktozamin ölçüm yöntemini otoanalizöre uyarlamak ve optimize etmek; daha sonra serum fruktozamin seviyesi ile uzun dönemli komplikasyonlar arasındaki ilişkiyi araştırmaktır.
YÖNTEM ve GEREÇLER: Yaşları ortalama 60 ± 20 sağlıklı kontroller (n = 146) ile yaşları ortalama 61 ± 11 diyabetik deneklerde (n = 353) serum fruktozamin ve HbA1c seviyeleri ölçüldü. Fruktozamin ölçümü için “Nitrobluetetrazolium yöntemi” otoanalizöre uyarlandı ve diformazanın yeterli çözünmesinin sağlanması ile ürik asit interferansının matematiksel düzeltmesi ile optimizasyonu sağlandı. Otomatize yönetimin gün içi ve günler arası tekrarlanabilirliği sırasıyla %0.7 (n=20) ve %2.5 (n=20) olarak saptandı.
BULGULAR: Ortalama fruktozamin konsantrasyonu DM’lerde (342 ± 74 μmol/L) kontrol grubuna (270 ± 47 μmol/L) göre belirgin yüksekti (p <0.01). Bu kesitsel çalışma, DM’un uzun dönemli komplikasyonlarıyla ilgili olmadığını gösterdi (p >0.05). Bununla beraber HbA1c ile koreledir (r = 0.817, p <0.01).
TARTIŞMA ve SONUÇ: Fruktozamin testi; ucuz, otomasyona uygun ve basit bir test olması yanında, gliseminin izlenmesinde yararlı bir laboratuvar parametresidir. Fakat fruktozamin seviyesi ile uzun dönemli komplikasyonlar arasındaki ilişkiyi belirlemek için prospektif çalışmalara ihtiyaç vardır.

Keywords

Tip 2 diyabetes mellitus, diyabetik komplikasyonlar, nonenzimatik glikozilasyon, glisemik kontrol, glikozile serum proteinleri, fruktozamin

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