Determining the effect of an electromagnetic field generated by a high voltage power line on rat spermatogonia cells

Year 2018, Volume: 45 Issue: 4, 447 - 461, 13.12.2018

Mehmet Cihan Yavaş , Veysi Akpolat Engin Deveci Hakki Murat Bilgin İbrahim Kaplan Ugur Seker , İsmail Yildiz Esref Alkis Mustafa Salih Celik Mehmet Zülküf Akdağ

https://doi.org/10.5798/dicletip.497923

Cited By: 3

Abstract

Objective: Purpose
of the study was to research the effect of an electromagnetic field created by
a high voltage line on the testes tissue and the serum biochemistry of Wistar
albino male rats. At the same time, in the study also examined the protective
effects of melatonin and ganoderma lucidum.

Methods: In
the study, 64 rats were divided into eight equal groups (n: 8). In experiments
lasting 26 and 52 days, the following groups were used: High voltage (HV), HV +
Ganoderma lucidum (GI), HV + Melatonin (MEL), and Sham-control. MEL (10 mg/kg)
was administered intraperitoneally and GI (20 mg/kg) as oral lavage. Extremely
low frequency electric (80.3 V/m) and magnetic fields (2.48 µT) were applied
for eight hours per day.

Results:
The biochemical results of the study show an increase in total oxidant status (TOS)
and oxidative stress index (OSI) level and a decrease in total antioxidant
status (TAS) level in the 26 and 52 day high voltage groups, compared to the
control group. In the high voltage groups, the nitric oxide (NO) value
increased with the increase of the exposure period. Testicular mass (p>0.05),
TAS, TOS, OSI and NO (p<0.05) were found at 26 days of comparison. Testicular
weight, TAS, TOS, OSI (p<0.05), and NO (p>0.05) were found in 52 day
comparisons. In routine histopathology data, we detected effects on both the 26
and 52 day HV groups, such as degeneration in spermatic cells and full
structural deterioration and increasing hyalinisation. We also detected an step
up in the ligament cell in the tubular field for the 52-day test groups. In
melatonin added group, proliferating cell nuclear antigen (PCNA)-positive cells
were more active. TUNEL assay analysis showed that there was no significant
difference between the the 26 day experiment groups (p>0.05), but that there
was a significant dissimilarity between the 52 day experiment groups
(p<0.05).

Conclusions:
In our study, the oxidative effect of created by a high voltage results in
changes in serum biochemistry of rats and a number of degenerative
deterioration in the histological structure of testes. Aslo, it is shown that
the oxidative effect created by a high voltage has negative effects on the creation
of spermatogonia. GI partly protects against these effects, while melatonin is
more effective in this regard.

Keywords

Melatonin, ganoderma, high voltage, electromagnetic field, spermatogonia, antioxidant

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