The protective effects of omega-3 fatty acids on rat testicular tissue

Year 2011, Volume: 38 Issue: 4, 382 - 386, 01.12.2011

İsmail Zararsız İlter Kuş Mürsel Davarcı Murat Abdulgani Kuş Dilara Kaman Mustafa Sarsılmaz

https://doi.org/10.5798/diclemedj.0921.2011.04.0054

Cited By: 1

Abstract

Objectives: In this study, the protective effect of omega-3 fatty acids on testicular tissue was aimed to investigate at biochemical levels. Materials and methods: Totally, 16 adult male Wistar rats were divided into two groups. Rats in Group I were used as control and only saline was given by intragastric gavage. Rats in Group II, 400 mg/kg dose ω-3 fatty ac­ids were given daily by intragastric gavage. At the end of the six-week experimental period, all rats were killed by decapitation. The testicular tissue specimens taken from animals, superoxide dismutase, glutathione peroxidase, malondialdehyde, enzyme activities were measured spectrophotometrically. In addition, blood testosterone levels were examined. Results: In our study, ω-3 fatty acids in rats were given a statistically significant increase in the levels of superoxide dismutase, and glutathione peroxidase a statistically sig­nificant decrease in malondialdehyde levels were deter­mined when compared to control group. In addition, ω-3 fatty acids in rats given a statistically significant increase in blood testosterone levels were observed. Conclusion: We concluded that ω-3 fatty acid had fa­vorable effects in rat testis tissue by preventing oxidative damage and increasing the level of testosterone.

Keywords

ω-3 fatty acids, testicle, testosterone, rat

Omega-3 yağ asitlerinin sıçan testis dokusu üzerine koruyucu etkileri

Abstract

Amaç: Bu çalışmada testis dokusu üzerine omega-3 yağ asitlerinin koruyucu etkisi biyokimyasal düzeyde araştırıl­ması amaçlandı. Gereç ve yöntem: Toplam 16 adet Wistar-Albino cinsi erişkin erkek sıçan 2 gruba ayrılarak incelendi. Grup I\'deki sıçanlar kontrol grubu olarak alındı ve sadece intragastrik gavaj ile serum fizyolojik verildi. Grup II\'deki sıçanlara ise, 400 mg/kg dozundaki ω-3 yağ asiti intragastrik gavaj yo­luyla günlük olarak verildi. Altı haftalık deney süresi so­nunda tüm sıçanlar dekapitasyon yöntemiyle öldürüldü. Hayvanlardan alınan testis doku örneklerinde superoksit dismutaz, Glutatyon peroksidaz, malondialdehit aktivite­leri spektrofotometrik olarak tayin edildi. Ayrıca kan tes­tosteron düzeyleri incelendi. Bulgular: Çalışmamızda, ω-3 yağ asidi verilen sıçanlar­da superoksit dismutaz, Glutatyon peroksidaz düzeyleri istatistiksel olarak anlamlı bir şekilde artarken, malondial­dehit düzeylerinde kontrol grubuna göre istatistiksel ola­rak anlamlı bir düşüş tespit edildi. Ayrıca omega-3 yağ asidi verilen sıçanların kan testosteron düzeylerinde ista­tistiksel olarak anlamlı bir artış görüldü. Sonuç: Sonuç olarak omega-3 yağ asitlerinin sıçan tes­tis dokusunda gerek oksidatif hasarı önleyerek, gerekse testosteron düzeyini artırarak olumlu etki gösterdiği tespit edildi.

Keywords

ω-3 yağ asiti, testis, testosteron, sı­çan

References

• Nordoy A. Is there a rational use for n-3 fatty acids (fish oils) in clinical medicine? Drugs 1991; 42(3):331-42.
• Bourre JM, Bonneil M, Chaudiere J, Clement M, Dumont O, Durand G. Structural and functional importance of dietary polyunsaturated fatty acids in the nervous system. Adv Exp Med Biol 1992;318(1):211-29.
• Masters C. Omega-3 fatty acids and the peroxisome. Mol Cell Biochem 1996;165(2):83-93.
• Zararsız İ, Kuş İ, Akpolat N, Songur A, Ögetürk M, Sarsılmaz M. Protective Effects of Omega-3 Essential Fatty Acids Against Formaldehyde-Induced Neuronal Damage in Prefrontal Cortex of Rats. Cell Biochem Funct 2006;.24(3):237-44.
• Zararsiz I, Sonmez MF, Yilmaz HR, et al. Effects of omega-3 essential fatty acids against formaldehyde induced neph- ropathy in rats. Toxicol Ind Health 2006b;22(5):223-9.
• Gamoh S, Hashimoto M, Sugioka K, Shahdat Hossain M, Hata N, Misawa Y, Masumura S. Chronic administra- tion of docosahexaenoic acid improves reference mem- ory-related learning ability in young rats. Neuroscience 1999;93(1):237-41.
• Zararsız İ, Sönmez MF, Yılmaz HR, Pekmez H, Kuş İ, Sarsıl- maz M. Sıçanlarda formaldehit uygulamasıyla akciğerlerde oluşan hasar üzerine omega-3 yağ asitlerinin koruyucu et- kisi. Selçuk Tıp Dergisi 2004;20(3):93-8.
• Sarsılmaz M, Songur A, Ozyurt H, et al. Potential role of di- etary ω-3 essential fatty acids on some oxidant/antioxidant parameters in rats’ corpus striatum. Prostaglandins Leukot Essent Fatty Acids 2003;69(4):253-9.
• Wills E D. The role of Dietary Components in Oxidative Stress in Tissues. Sies H (Ed.). Academic Press. Orlando, 1985; s: 197-220.
• Yılmaz, H.R, Songur A, Özyurt B, Zararsız İ, Sarsılmaz M. The effects of n-3 polyunsaturated fatty acids by gavage on some metabolic enzymes of rat liver. Prostaglandins Leukot Essent Fatty Acids 2004;71(2):131-5.
• Parker G, Gibson N.A, Brotchie H, Heruc G, Rees A.M, Pavlovic D.H. Omega-3 Fatty Acids and Mood Disorders. Am J Psychiatry 2006;163(6): 969-78.
• Saravanan P, Davidson NC, Schmidt EB, Calder PC. Car- diovascular effects of marine omega-3 fatty acids. The Lan- cet 2010; 376 (9740): 540-50.
• Comhaire FH, Mahmoud A. The role of food supplements in the treatment of the infertile man. Reprod Biomed Online 2003;7(4): 385-91.
• Esterbauer H, Cheeseman KH. Determination of alde- hydic lipid peroxidation products: malonaldehyde and 4-hidroxynonenal. Methods Enzymol 1990;186(1):407-21.
• Sun Y, Oberley LW, Li Y. A simple method for clinical assay of superoxide dismutase. Clin Chem 1988; 34(3):497-500.
• Paglia DE, Valentine WN. Studies on the quantitative and qualitative characterisation of erythrocyte glutathione per- oxidase. J Lab Clin Med 1967;70(1): 158-69.
• Stone NJ. Fish comsumption, fish oil, lipids, and coronary heart disease. Am J Clin Nutr 1997;65(4):1083-6.
• Miyasaka CK, de Souza JA, Torres RP, Filho JM, Lajolo FM, Curi R. Effect of the administration of fish oil by gav- age on activities of antioxidant enzymes of rat lymphoid organs. Gen Pharmacol 1998;30(5):759-62.
• Hallivel B. Free radicals, antioxidants and human dis- ease; curiosity, course or consequence? Lancet 1994; 344 (8924):721-4.
• Zararsız İ, Meydan S, Sarsılmaz M, Songur A, Ozen OA, Sogut S. Protective effects of omega-3 essential fatty acids against formaldehyde-induced cerebellar damage in rats. Toxicol Ind Health 2011;27(6):489-95.
• Akyol Ö. Beyin tümörlerinde doku SOD, CAT ve GSH-Px aktiviteleri. Uzmanlık tezi, Ankara: Ankara Üniversitesi Tıp Fakültesi, Biyokimya Bölümü, Ankara 1994.
• Kus İ, Zararsız İ, Ögetürk M, Yılmaz H.R, Sarsılmaz M. Deneysel formaldehit toksisitesinde testis SOD, GSH-Px, MDA düzeyleri ve ω-3 yağ asitlerinin koruyucu etkisi. Fırat Tıp Dergisi 2008;13(1): 10-4.
• Pompella A. Biochemistry and histochemistry of oxi- dant stress and lipid peroxidation. Int J Vitam Nutr Res 1997;67(5): 289-97.