THE CYTOTOXIC EFFECTS OF PROTEASOME INHIBITOR MG132 ON 4T1 BREAST AND B16F10 MELANOMA CANCER CELLS

Year 2009, Issue: 019, 15 - 22, 15.08.2009

Azmi Yerlikaya Harun Dokudur Semih Şeker

Abstract

Proteasome
is a proteolytic complex found in all eukaryotes, archaeabacteria and some
eubacteria. Increases in proteasome activity and subunits are commonly found in
many cancers. Proteasome inhibitors exhibits antitumor activities in a number
of cancer cell cultures by triggering apoptosis. Studies are currently
conducted  in many laboratories in order
to use proteasome inhibitors in cancer treatment. In this study, we
investigated the cytotoxic effects of proteasome inhibitor MG132 on 4T1 breast
and B16F10 melanoma cancer cells. It was determined that both cell lines are
sensitive to MG132 using morphologic criteria commonly found in apoptotic cells
and typan blue exclusion test. Results suggest that the proteasome inhibitors
may show effective anti-cancer effects against melonoma and breast cancers and
are therefore warranted to further investigation. 

Keywords

Proteasome, MG132, cancer, cytotoxic effects

PROTEOZOM İNHİBİTÖRÜ MG132’NİN 4T1 MEME VE B16F10 MELANOMA KANSER HÜCRELERİNDEKİ SİTOTOKSİK ETKİLERİ

Abstract

Proteozom,
tüm ökaryotlar, arkeobakteriler ve bazı bakterilerde bulunan proteolitik
aktiviteye sahip bir komplekstir. Çoğu kanserde proteozom aktivitesinde ve
alt-birimlerinde yükselmeler sıklıkla görülmektedir. Proteozom inhibitörleri
kanser hücre kültürlerinde apoptozisi uyararak anti-tümör aktivite
göstermektedirler. Proteozom inhibitörlerini kanser tedavisinde kullanmak için
çalışmalar birçok laboratuarda halen devam etmektedir. Bu çalışmada proteozom
inhibitörü MG132’nin 4T1 meme ve B16F10 melanoma kanser hücrelerindeki
sitotoksik etkileri araştırıldı. Apoptotik hücrelerde yaygın olarak kullanılan
morfolojik kriterler ve tripan mavisi testi ile her iki hücre kültürünün
MG132’ye hassas olduğunu belirledik. Bulgular, proteozom inhibitörlerinin
melanoma ve meme kanseri hücrelerine karşı anti-kanser etki
gösterebileceklerini ve bundan dolayı daha ileri düzeyde araştırılması
gerektiğini önermektedir.

Keywords

Proteozom, MG132, kanser, sitotoksik etki

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