Year 2020, Volume 8 , Issue 2, Pages 1250 - 1257 2020-04-30

Leucoagaricus leucothites’in In Vitro Antimikrobiyal Aktivite Taraması ve Etanol Ekstrakt Kompozisyonunun Gaz Kromatografisi/Kütle Spektrometresi ile Belirlenmesi
In Vitro Antimicrobial Activity Screening of Leucoagaricus leucothites and Determination of the Ethanol Extract Composition By Gas Chromatography/Mass Spectrometry

Kerem CANLI [1] , İlgaz AKATA [2] , Ali YETGİN [3] , Atakan BENEK [4] , Ergin Murat ALTUNER [5]


Makro mantarlar sadece tıbbi özelliklere sahip olmayan, aynı zamanda da besleyici olan iyi besin kaynaklarıdır. Yüzyıllar boyunca kanser, hiperkolesterolemi ve hipertansiyon gibi çeşitli hastalıkları önlemek için kullanılmışlardır. Ayrıca makro mantarların antimikrobiyal aktivite gösterdikleri de bilinmektedir, bu nedenle bu çalışmanın ana amacı, bir makro mantar olan Leucoagaricus leucothites (Vittad.) Wasser 1977’nin etanol ekstraktının antimikrobiyal aktivitesini ortaya koymak ve ayrıca Gaz Kromatografisi-Kütle Spektrometresi ile kimyasal bileşimin belirlenmesini sağlamaktır. Antimikrobiyal taramada on dokuz bakteri, bir adet maya suşu kullanılmıştır. Bu çalışmadan elde edilen verilere göre L. leucothites farklı konsantrasyonlarda kullanılan tüm mikroorganizmalara karşı farklı seviyelerde hem antibakteriyel hem de antifungal aktivite ortaya koymuştur. Her ne kadar daha önce yapılmış birkaç çalışma bulunsa da; bu çalışmanın, L. leucothites'in antibakteriyel ve antifungal potansiyeli ve L. leucothites etanol ekstraktında bulunan bileşikler ile ilgili ilk ayrıntılı tarama raporu olduğu öne sürülebilir.

Macrofungi are good food resources, which have not only medicinal properties but are nutritive as well. For centuries they have been used for preventing several diseases including cancer, hypercholesterolemia and hypertension. They are also known to present antimicrobial activity, thus the aim of the present study is to put forward antimicrobial activity of ethanol extract of Leucoagaricus leucothites (Vittad.) Wasser 1977, a macro fungi and in addition to determine the chemical composition by Gas Chromatography-Mass Spectrometry. Nineteen bacteria strains and one yeast strain are used in antimicrobial screening. According to the data obtained from the study L. leucothites presented both antibacterial and antifungal activity against the bacteria and yeast strains used at different concentrations with different levels. Although there are some previous studies, it can be proposed that this study is the first detailed screening report regarding the antibacterial and antifungal potential of L. leucothites and the compounds found in L. leucothites ethanol extract.

  • [1] F. Watanabe, Y. Yabuta, T. Bito, and F. Teng, “Vitamin B12-Containing plant food sources for vegetarians,” Nutrients, vol. 6, no. 5, pp. 1861-1873, 2014.
  • [2] G. Cardwell, J. Bornman, A. James, and L. Black, “A review of mushrooms as a potential source of dietary vitamin D,” Nutrients, vol. 10, no. 10, pp. 1498, 2018.
  • [3] J. Falandysz, “Selenium in Edible Mushrooms,” J. Environ. Sci. Heal. C, vol. 26, no. 3, pp. 256-299, 2008.
  • [4] M. Bonatti, P. Karnopp, H. M. Soares, and S. A. Furlan, “Evaluation of Pleurotus ostreatus and Pleurotus sajor-caju nutritional characteristics when cultivated in different lignocellulosic wastes,” Food Chem., vol. 88, no. 3, pp. 425-428, 2004.
  • [5] D. Agrahar-Murugkar and G. Subbulakshmi, “Nutritional value of edible wild mushrooms collected from the Khasi Hills of Meghalaya,” Food Chem., vol. 89, no. 4, pp. 599-603, 2005.
  • [6] L. M. Cheung and P. C. Cheung, “Mushroom extracts with antioxidant activity against lipid peroxidation,” Food Chem., vol. 89, no. 3, pp. 403-409, 2005.
  • [7] A. Imtiaj and T. S. Lee, “Screening of antibacterial and antifungal activities from Korean wild mushrooms,” World J Agric. Sci., vol. 3, no. 3, pp. 316-321, 2007.
  • [8] J. Clardy and C. Walsh, “Lessons from natural molecules,” Nature, vol. 432, no. 7019, pp. 829-837, 2004.
  • [9] D. Webster, P. Taschereau, R.J. Belland, C. Sand, and R. P. Rennie, “Antifungal activity of medicinal plant extracts; preliminary screening studies,” J. Ethnopharmacol., vol. 115, no. 1, pp. 140-146, 2008.
  • [10] K. Canli, E. M. Altuner, I. Akata, Y. Turkmen, and U. Uzek, “In vitro antimicrobial Screening of Lycoperdon lividum and determination of the ethanol extract composition by gas chromatography/mass spectrometry,” Bangladesh J. Pharmacol., vol. 11, no. 2, pp. 389-394, 2016.
  • [11] K. Canli, I. Akata, and E. M. Altuner, “In vitro antimicrobial activity screening of Xylaria hypoxylon,” Afr. J. Tradit. Complement. Altern. Med., vol. 13, no. 4, pp. 42-46, 2016.
  • [12] WHO. (2019, Aug 15). Antibiotic resistance [Online]. Available: https://www.who.int/news-room/fact-sheets/detail/antibiotic-resistance.
  • [13] B. Paudel, H. D. Bhattarai, J. S. Lee, S. G. Hong, H. W. Shin, and J. H. Yim, “Antibacterial potential of antarctic lichens against human pathogenic gram‐positive bacteria,” Phytother. Res., vol. 22, no. 9, pp. 1269-1271, 2008.
  • [14] E. M. Altuner, K. Canli, and I. Akata, “Antimicrobial screening of Calliergonella cuspidata, Dicranum polysetum and Hypnum cupressiforme,” J. Pure Appl. Microbio., vol. 8, no. 1, pp. 539-545, 2014.
  • [15] N. Bala, E. A. Aitken, N. Fechner, A. Cusack, and K. J. Steadman, “Evaluation of antibacterial activity of Australian basidiomycetous macrofungi using a high-throughput 96-well Plate assay,” Pharm. Biol., vol. 49, no. 5, pp. 492-500, 2011.
  • [16] B. Dülger, F. Şen, and F. Gücin, “Antimicrobial activity of the macrofungi Russula delica Fr.,” Turkish J. Biol., vol. 23, no. 1, pp. 127-134, 1999.
  • [17] N. Gunde-Cimerman, “Medicinal value of the genus Pleurotus (Fr.) P. Karst.(Agaricales Sl, Basidiomycetes),” Int. J. Med. Mushrooms, vol. 1, no. 1, pp. 69-80, 1999.
  • [18] V. E. C. Ooi, “Medicinally important Fungi,” in Science and Cultivation of Edible Fungi, V. Griensven, Ed., Rotterdam: Balkema, 2010, pp. 41-51.
  • [19] S. P. Wasser and A. L. Weis, “Medicinal properties of substances occurring in higher basidiomycetes mushrooms: current perspectives,” Int. J. Med. Mushrooms, vol. 1, no. 1, pp. 31-62, 1999.
  • [20] S. P. Wasser and A. L. Weis, “Therapeutic effects of substances occurring in higher basidiomycetes mushrooms: a modern perspective,” Crit. Rev. Immunol., vol. 19, no. 1, pp. 65-96, 1999.
  • [21] B. Dulger, T. B. Suerdem, D. Yesilyurt, N. Hacioglu, and A. Camdeviren, “Evaluation of antimicrobial activity of the macrofungus Phellinus torulosus,” J. Biol. Sci., vol. 5, no. 4, pp. 436-439, 2005.
  • [22] E. M. Altuner and K. Canli, “In vitro antimicrobial screening of Hypnum andoi AJE Sm,” Kastamonu Üniv. Orman Fak. Derg., vol. 12, no. 1, pp. 97-101, 2012.
  • [23] E. M. Altuner, “Bazı karayosunu türlerinin antimikrobiyal aktivitesinin belirlenmesi”, Doktora tezi, Ankara Üniversitesi, Ankara, Türkiye, 2008.
  • [24] K. A. Hammer, C. F. Carson, and T. V. Riley, “Antimicrobial activity of essential oils and other plant extracts,” J. Appl. Microbiol., vol. 86, no. 6, pp. 985-990, 1999.
  • [25] E. M. Altuner, I. Akata, and K. Canli, “In vitro antimicrobial activity screening of Bovista nigrescens Pers,” Kastamonu Üniv. Orman Fak. Derg., vol. 12, no. 1, pp. 90-96, 2012.
  • [26] K. Canli, A. Yetgin, I. Akata, and E. M. Altuner, “In vitro antimicrobial screening of Aquilaria agallocha roots,” Afr. J. Tradit. Complement. Altern. Med., vol. 13, no. 5, pp. 178-181, 2016.
  • [27] J. M. Andrews, “BSAC standardized sisc susceptibility testing method (Version 6),” J. Antimicrob. Chemother., vol. 60, no. 1, pp. 20-41, 2007.
  • [28] A. M. Mahasneh and A. A. El-Oqlah, “Antimicrobial activity of extracts of herbal plants used in the traditional medicine of Jordan,” J. Ethnopharmacol., vol. 64, no. 3, pp. 271-276, 1999.
  • [29] S. Silici and A. N. Koc, “Comparative study of in vitro methods to analyse the antifungal activity of propolis against yeasts isolated from patients with superficial mycoses,” Lett. Appl. Microbiol., vol. 43, no. 3, pp. 318-324, 2006.
  • [30] M. A.Hossain and A. Rahman, “Chemical composition of bioactive compounds by gc-ms screening and anti-fungal aroperties of the crude extracts of cabbage samples,” Asian J. Biotechnol., vol. 3, pp. 68-76, 2011.
  • [31] K. Canli, A. Yetgin, I. Akata, and E. M. Altuner, “Antimicrobial activity and chemical composition screening of Anacyclus pyrethrum Root,” Indian J. Pharm. Educ., vol. 51, pp. 244-248, 2017.
  • [32] K. Canli, A. Yetgin, I. Akata, and E. M. Altuner, “Antimicrobial activity and chemical composition screening of Epilobium montanum Root,” Indian J. Pharm. Educ., vol. 51, pp. 239-243, 2017.
  • [33] Core R Team. (2019, Aug 15). R: A language and environment for statistical computing [Online]. Available: https://www.R-project.org/.
  • [34] C. S. Kalaivani, S. S. Sathish, N. Janakiraman, and M. Johnson, “Gc-ms studies on Andrographis paniculata (Burm. f.) Wall. Ex Nees-a medicinally important plant,” Indian Int. J. Med. Arom. Plants, vol. 2, no.1, pp. 69-74, 2012.
  • [35] M. M. Rahman, S. H. Ahmad, M. T. M. Mohamed, and M. Z. Ab Rahman, “Antimicrobial compounds from leaf extracts of Jatropha curcas, Psidium guajava, and Andrographis paniculata,” Sci. World J., 2014, Art no. 635240.
  • [36] C. B. Huang, B. George, and J. L. Ebersole, “Antimicrobial activity of n-6, n-7 and n-9 Fatty acids and their esters for oral microorganisms,” Arch. Oral. Biol., vol. 55, no. 8, pp. 555-560, 2010.
  • [37] C. S. Lunde, S. R. Hartouni, J. W. Janc, M. Mammen, P. P. Humphrey, and B. M. Benton, “Telavancin disrupts the dunctional integrity of the bacterial membrane through targeted interaction with the cell wall precursor lipid II,” Antimicrob. Agents Chemother., vol. 53, no. 8, pp. 3375-3383, 2009.
  • [38] B. Aslim and S. Ozturk, “Phenolic composition and antimicrobial and antioxidant activities of Leucoagaricus leucothites (Vittad.) Wasser,” J. Med. Food, vol. 14, no. 11, pp. 1419-1424, 2011.
  • [39] M. Sevindik, A. Rasul, G. Hussain, H. Anwar, M. K. Zahoor, I. Sarfraz, K S. Kamran, H. Akgul, I. Akata, and Z. Selamoglu, “Determination of anti-oxidative, anti-microbial activity and heavy metal contents of Leucoagaricus leucothites,” Pak. J. Pharm. Sci., vol. 31, no. 5, pp. 2163-2168, 2018.
Primary Language en
Subjects Engineering
Journal Section Articles
Authors

Orcid: 0000-0001-6061-6948
Author: Kerem CANLI (Primary Author)
Institution: DOKUZ EYLUL UNIVERSITY, FACULTY OF SCIENCE, DEPARTMENT OF BIOLOGY
Country: Turkey


Orcid: 0000-0002-1731-1302
Author: İlgaz AKATA
Institution: ANKARA UNIVERSITY, FACULTY OF SCIENCE, DEPARTMENT OF BIOLOGY
Country: Turkey


Orcid: 0000-0001-7683-8836
Author: Ali YETGİN
Institution: Toros Tarım Sanayi ve Ticaret A.Ş.
Country: Turkey


Orcid: 0000-0001-6726-5968
Author: Atakan BENEK
Institution: KASTAMONU UNIVERSITY, FACULTY OF SCIENCE AND LETTERS, DEPARTMENT OF BIOLOGY
Country: Turkey


Orcid: 0000-0001-5351-8071
Author: Ergin Murat ALTUNER
Institution: KASTAMONU UNIVERSITY, FACULTY OF SCIENCE AND LETTERS, DEPARTMENT OF BIOLOGY
Country: Turkey


Dates

Publication Date : April 30, 2020

Bibtex @research article { dubited642707, journal = {Düzce Üniversitesi Bilim ve Teknoloji Dergisi}, issn = {}, eissn = {2148-2446}, address = {}, publisher = {Duzce University}, year = {2020}, volume = {8}, pages = {1250 - 1257}, doi = {10.29130/dubited.642707}, title = {In Vitro Antimicrobial Activity Screening of Leucoagaricus leucothites and Determination of the Ethanol Extract Composition By Gas Chromatography/Mass Spectrometry}, key = {cite}, author = {Canlı, Kerem and Akata, İlgaz and Yetgi̇n, Ali and Benek, Atakan and Altuner, Ergin Murat} }
APA Canlı, K , Akata, İ , Yetgi̇n, A , Benek, A , Altuner, E . (2020). In Vitro Antimicrobial Activity Screening of Leucoagaricus leucothites and Determination of the Ethanol Extract Composition By Gas Chromatography/Mass Spectrometry . Düzce Üniversitesi Bilim ve Teknoloji Dergisi , 8 (2) , 1250-1257 . DOI: 10.29130/dubited.642707
MLA Canlı, K , Akata, İ , Yetgi̇n, A , Benek, A , Altuner, E . "In Vitro Antimicrobial Activity Screening of Leucoagaricus leucothites and Determination of the Ethanol Extract Composition By Gas Chromatography/Mass Spectrometry" . Düzce Üniversitesi Bilim ve Teknoloji Dergisi 8 (2020 ): 1250-1257 <https://dergipark.org.tr/en/pub/dubited/issue/55096/642707>
Chicago Canlı, K , Akata, İ , Yetgi̇n, A , Benek, A , Altuner, E . "In Vitro Antimicrobial Activity Screening of Leucoagaricus leucothites and Determination of the Ethanol Extract Composition By Gas Chromatography/Mass Spectrometry". Düzce Üniversitesi Bilim ve Teknoloji Dergisi 8 (2020 ): 1250-1257
RIS TY - JOUR T1 - In Vitro Antimicrobial Activity Screening of Leucoagaricus leucothites and Determination of the Ethanol Extract Composition By Gas Chromatography/Mass Spectrometry AU - Kerem Canlı , İlgaz Akata , Ali Yetgi̇n , Atakan Benek , Ergin Murat Altuner Y1 - 2020 PY - 2020 N1 - doi: 10.29130/dubited.642707 DO - 10.29130/dubited.642707 T2 - Düzce Üniversitesi Bilim ve Teknoloji Dergisi JF - Journal JO - JOR SP - 1250 EP - 1257 VL - 8 IS - 2 SN - -2148-2446 M3 - doi: 10.29130/dubited.642707 UR - https://doi.org/10.29130/dubited.642707 Y2 - 2020 ER -
EndNote %0 Düzce Üniversitesi Bilim ve Teknoloji Dergisi In Vitro Antimicrobial Activity Screening of Leucoagaricus leucothites and Determination of the Ethanol Extract Composition By Gas Chromatography/Mass Spectrometry %A Kerem Canlı , İlgaz Akata , Ali Yetgi̇n , Atakan Benek , Ergin Murat Altuner %T In Vitro Antimicrobial Activity Screening of Leucoagaricus leucothites and Determination of the Ethanol Extract Composition By Gas Chromatography/Mass Spectrometry %D 2020 %J Düzce Üniversitesi Bilim ve Teknoloji Dergisi %P -2148-2446 %V 8 %N 2 %R doi: 10.29130/dubited.642707 %U 10.29130/dubited.642707
ISNAD Canlı, Kerem , Akata, İlgaz , Yetgi̇n, Ali , Benek, Atakan , Altuner, Ergin Murat . "In Vitro Antimicrobial Activity Screening of Leucoagaricus leucothites and Determination of the Ethanol Extract Composition By Gas Chromatography/Mass Spectrometry". Düzce Üniversitesi Bilim ve Teknoloji Dergisi 8 / 2 (April 2020): 1250-1257 . https://doi.org/10.29130/dubited.642707
AMA Canlı K , Akata İ , Yetgi̇n A , Benek A , Altuner E . In Vitro Antimicrobial Activity Screening of Leucoagaricus leucothites and Determination of the Ethanol Extract Composition By Gas Chromatography/Mass Spectrometry. DÜBİTED. 2020; 8(2): 1250-1257.
Vancouver Canlı K , Akata İ , Yetgi̇n A , Benek A , Altuner E . In Vitro Antimicrobial Activity Screening of Leucoagaricus leucothites and Determination of the Ethanol Extract Composition By Gas Chromatography/Mass Spectrometry. Düzce Üniversitesi Bilim ve Teknoloji Dergisi. 2020; 8(2): 1250-1257.