j du health sci inst Düzce Üniversitesi Sağlık Bilimleri Enstitüsü Dergisi 2146-443X Duzce University 10.33631/duzcesbed.562838 Health Care Administration Sağlık Kurumları Yönetimi Düzce İlinde Koyunlarda Coxiella burnetii ve Chlamydia abortus Etkenlerine karşı Antikorların Araştırılması Investigation of Coxiella burnetii and Chlamydia abortus Antibodies in Sheep in Düzce Region https://orcid.org/0000-0001-7215-5229 Karagül Mustafa Sencer Kocaeli University Kartepe Vocational School of Equine Science https://orcid.org/0000-0001-8656-3354 Malal Mehmet Engin Pendik Veterinary Control Institute https://orcid.org/0000-0003-0894-7357 Akar Kadir Pendik Veterinary Control Institute 09 30 2019 9 3 106 109 05 10 2019 06 24 2019 Copyright © 2010, Journal of Duzce University Health Sciences Institute 2010 Journal of Duzce University Health Sciences Institute

Amaç: Bu çalışmanınamacı Düzce ilindeki koyunlarda, Coxiellaburnetii ve Chlamydia abortusetkenine karşı spesifik antikorları araştırmaktır. Q humması ve koyunlarınenzootik abortusu hastalıklarının ayrı ayrı nedeni olan söz konusu 2 patojen,küçük ruminantlarda yavru atmalar ile ilişkilidir. Mikrobiyal etkenlerin nedenolduğu yavru atmalara bağlı ekonomik kayıplar dünyanın birçok ülkesinde kaydadeğer seviyededir.Gereç veYöntemler:Çalışma kapsamında serolojik testler için koyun kan örnekleri Düzce ilindentoplandı. Ticari ELISA kitleri, C.burnetii ve C. abortusetkenlerine karşı spesifik antikorların tespiti için kullanıldı. Düzce ilinin 5ilçesindeki 12 köy veya mahallede yer alan 14 sürüden alınmış 72 numuneçalışmada kullanıldı. Numuneler, 2-5 yaş aralığında yavru atma geçmişi olmayandişi hayvanlardan alındı.Bulgular: Sırasıylakoyunların enzootik abortusu ve Q humması hastalıkları için total seroprevalans%20,83 ve %26,38 olarak bulundu. Fakat seropozitif sürü oranı, totalseroprevalansdan daha yüksek oranda sırasıyla koyunların enzootik abortusu ve Qhumması hastalıkları için %42,8 ve %50 olarak bulundu. 14 sürü içinde 7 sürüdeher iki hastalık için seropozitif hayvanlar tespit edildi.Sonuç: Çalışmasonuçları Düzce ilindeki araştırılan koyun sürülerinin C. burnetii ve C. abortusetkenlerine maruz kaldığını doğrulamaktadır. Elde edilen verilere göre, Düzceilinde koyun yavru atma vakalarında çalışmada araştırılan iki abort etkeninmuhtemel bağlantısının değerlendirilmesi faydalı olacaktır.

Aim: The aimof this study is to investigate the Coxiellaburnetii and Chlamydia abortusantibodies in sheep in Düzce province. These two pathogens are causative agentsof Query fever and Enzootic abortion of ewes, respectively which are related toabortion in small ruminants. Economic loss due to the abortions which arecaused by microbiological agents is considerably high in many countries. Material and Methods: Blood samples of sheep were collected from Düzce province of Turkey forserological tests. Commercial enzyme-linked immunosorbent assay (ELISA) wasused for the detection of specific antibodies to C. burnetii and C. abortus.A total of 72 samples obtained from 14 herds located in 12 villages/districtsof 5 towns in Düzce province were utilized in this study. Samples werecollected from 2-5-year-old female animals without an abortion history.Results: Total seroprevalenceof Enzootic abortion of ewes and Query fever was found to be 20.83% and 26.38%,respectively. However, the proportion of seropositive herds was found to be42.8% for Enzootic abortion of ewes and 50% for Q fever, which is higher thantotal seroprevalence. 6 flocks out of 14 were found to be seropositive for bothof the diseases simultaneously.Conclusion: This study confirms the exposure of sheep herds in Düzce province to C. burnetii and C. abortus and provides original seroprevalence data about Düzce.The data gathered are beneficial to evaluate the possible connection of twoinvestigated abortogenic pathogens in sheep abortion cases in Düzce province.

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