Mutagenic Potentials of Self-Adhesive Luting Cements as Detected by the Salmonella/Microsome Test

Year 2015, Volume: 42 Issue: 2 - Volume: 42 Issue: 2, 95 - 102, 01.08.2015

Tuğba Toz Arlin Kiremitçi Zeliha Aydoğan

Abstract

Resin cements have been considered the material of choice to cement indirect restorations, may be contact with tooth and oral tissues for prolonged periods of time. Thus, assessments of these materials’ biocompatibility is gaining increasing importance for both patients and dentists. The aim of study was to evaluate the potential mutagenicity of two self-adhesive luting cement “Biscem” Bisco, USA and “Maxcem” Kerr, Switzerland by employing the Ames Salmonella/mikrosome test. The materials were eluted in dimethyl sulphoxide and the aliquots were used and incubation period of one day at 370 C. Mutagenic effects of the materials were tested on Salmonella typhimurium strains TA 98 and TA 100 using the standard plate incorporation assay in the absence of S9 fraction from rat liver. No mutagenic effects were detected for the two self adhesive luting cements tested P >0,05 . We can conclude that Biscem and Maxcem has no mutagenic effect, but there is need for further investigations on different test strains, test aliquots and incubation periods and also in the presence of S9 fraction

Self adeziv yapıştırıcı simanların mutajenik potansiyellerinin salmonella/mikrozom testi ile değerlendirilmesi

Abstract

İndirekt restorasyonların yapıştırılmasında tercih edilen self-adeziv yapıştırıcı simanlar, diş ve ağız dokuları ile uzun süre temas halindedirler. Bu nedenle bu materyallerin biyouyumlulukları hasta ve hekimler için artan bir şekilde önem kazanmaktadır. Bu çalışmanın amacı, iki self-adeziv yapıştırıcı simanın “Biscem” Bisco, ABD ve “Maxcem” Kerr, İsviçre mutajenik potansiyellerinin Ames Salmonella/mikrozom test sistemi ile değerlendirilmesidir. Kullanılan test materyallerinin ekstraktları dimetil sülfoksit içerisinde 1 gün boyunca 37C0 ‘de bekletilerek hazırlanmıştır. Materyallerin mutajenik etkileri TA98 ve TA100 test suşlarının kullanılması ile plak inkorporasyon yönteminin uygulanması sonucu S9 kullanılmadan test edilmiştir. Her iki self-adeziv yapıştırıcı siman için herhangi bir mutajenik etki gözlenmemiştir P >0.05 . Bu çalışmanın sınırları dahilinde self-adeziv yapıştırıcı simanlar Biscem ve Maxcem mutajenik etki göstermemişlerdir. Bununla birlikte; farklı test suşları, farklı çözücüler ve farklı ekstrakt sürelerinde ve ayrıca S9 varlığında yapılmış daha fazla araştırma gereksinimi vardır.

References

• 1. De Munck, J., et al., Bonding of an auto-adhesive luting material to enamel and dentin. Dent Mater, 2004. 20(10): p. 963-71.
• 2. Schweikl, H., et al., Inhibition of TEGDMA and HEMA-induced genotoxicity and cell cycle arrest by N-acetylcysteine. Dent Mater, 2007. 23(6): p. 688-95.
• 3. Heil, J., et al., Genotoxicity of dental materials. Mutat Res, 1996. 368(3-4): p. 181- 94.
• 4. Schweikl, H. and G. Schmalz, Triethylene glycol dimethacrylate induces large deletions in the hprt gene of V79 cells. Mutat Res, 1999. 438(1): p. 71-8.
• 5. Spagnuolo, G., et al., NF-kappaB protection against apoptosis induced by HEMA. J Dent Res, 2004. 83(11): p. 837-42.
• 6. Schweikl, H., G. Schmalz, and T. Spruss, The induction of micronuclei in vitro by unpolymerized resin monomers. J Dent Res, 2001. 80(7): p. 1615-20.
• 7. Mortelmans, K. and E. Zeiger, The Ames Salmonella/microsome mutagenicity assay. Mutat Res, 2000. 455(1-2): p. 29-60.
• 8. Maron, D.M. and B.N. Ames, Revised methods for the Salmonella mutagenicity test. Mutat Res, 1983. 113(3-4): p. 173-215.
• 9. Ames, B.N., Identifying environmental chemicals causing mutations and cancer. Science, 1979. 204(4393): p. 587-93.
• 10. Jorgensen, K.V., J.W. Clayton, and R.L. Price, Evaluation of aflatoxin B1 mutagenesis: addition of glutathione and glutathione-S-transferase to the Salmonella mutagenicity assay. Environ Mutagen, 1987. 9(4): p. 411-9.
• 11. Rosin, M.P. and H.F. Stich, Assessment of the use of the Salmonella mutagenesis assay to determine the influence of antioxidants on carcinogen-induced mutagenesis. Int J Cancer, 1979. 23(5): p. 722-7.
• 12. Levin, D.E. and B.N. Ames, Classifying mutagens as to their specificity in causing the six possible transitions and transversions: a simple analysis using the Salmonella mutagenicity assay. Environ Mutagen, 1986. 8(1): p. 9- 28.
• 13. Schweikl, H., G. Schmalz, and K. Rackebrandt, The mutagenic activity of unpolymerized resin monomers in Salmonella typhimurium and V79 cells. Mutat Res, 1998. 415(1-2): p. 119-30.
• 14. Kleinsasser, N.H., et al., Genotoxicity and cytotoxicity of dental materials in human lymphocytes as assessed by the single cell microgel electrophoresis (comet) assay. J Dent, 2004. 32(3): p. 229-34.
• 15. Arossi, G.A., et al., Induced DNA damage by dental resin monomers in somatic cells. Basic Clin Pharmacol Toxicol, 2010. 106(2): p. 124-9.
• 16. Suarez, S., R.A. Sueiro, and J. Garrido, Genotoxicity of the coating lacquer on food cans, bisphenol A diglycidyl ether (BADGE), its hydrolysis products and a chlorohydrin of BADGE. Mutat Res, 2000. 470(2): p. 221-8.
• 17. Poplawski, T., et al., Cytotoxicity and genotoxicity of glycidyl methacrylate. Chem Biol Interact, 2009. 180(1): p. 69-78.
• 18. Prica, D., et al., Genotoxicity evaluation of five different dentin bonding agents by chromosomal aberration analysis. J Oral Rehabil, 2006. 33(6): p. 462-71.