In vitro study on antioxidant, antibacterial and DNA interaction activities of extracts from Arbutus andrachne L.

Year 2019, Volume: 7 Issue: 3, 293 - 300, 29.10.2019

Ceren Baskan Dudu Duygu Kılıç Belgin Sırıken Güler Tanrikulu Melek Gül Ömer Ertürk

https://doi.org/10.31195/ejejfs.599543

Cited By: 1

Abstract

Traditionally, the fruits and leaves of Arbutus andrachne are well known and used in for applications in
antimicrobial, antioxidant and anticancer activity. This study was aimed to
determine the in vitro antibacterial,
antioxidant and DNA interaction activities of the different extracts of Arbutus andrachne.

Arbutus andrachne was collected from Amasya Province during
May 2018. Dried leaves powder were mixed with of suitable solution. Then, the
mixtures were extracted with the soxhlet apparatus for 4 hours. The
antibacterial effects of extracts were researched on pathogens, namely Staphylococcus aureus ATCC 25923, Staphylococcus aureus ATCC 25953, Escherichia coli ATCC 25922, Pseudomonas aeruginosa ATCC 27853, Pseudomonas aeruginosa ATCC 9027, Bacillus cereus ATCC 7064, Bacillus subtilis ATCC 6633, and Salmonella enteritidis ATCC 13076 using
disc diffusion methods. Gentamicin and cefotaxime were used as control for
bacteria. To try to understand each plant extracts effect on the antioxidant
methods, we studied the different methods as named radical scavenging activity
(DPPH), metal chelating activity, ferric reducing antioxidant power assays. In
the plant extracts, total phenolic and total flavonoid contents were determined
by spectrophotometric methods due to investigating the effect of secondary
metabolites on antioxidant activity. To explore the beneficial effect of the
extracts on hydroxyl radical-mediated DNA damage plasmid DNA pUC18 was used.

As a result, Arbutus andrachne
extracts had antibacterial effect when they compared with control group
antibiotics. According to disc diffusion methods, the highest antibacterial
effect was identified in ethanol, chloroform and hexane extract, respectively.
Ethanol extracts of Arbutus andrachne
showed the best antioxidant activity. Moreover, extracts of Arbutus andrachne had repair effects on
plasmid DNA in H₂O₂ condition.

The investigated Arbutus
andrachne extract showed significant bioactivities. These extracts have
been a promising candidate for the preparation of new natural products.
However, future studies should be carried out to verify such actions in
different matrices.

Keywords

Arbutus andrachne L. antioxidant, antimicrobial, DNA interaction

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