REVELATION AND DETERMINATION OF 3-MONOCHLOROPROPANE-1,2-DIOL (3-MCPD) IN BREAD FROM MODERN BAKERIES IN KHARTOUM STATE, SUDAN.

Year 2020, Volume: 4 Issue: 2, 70 - 81, 30.11.2020

Abdalbasit Marıod , Yousıf Idrıs

Abstract

This study was carried out for the revelation and determination of 3-Monochloropropan-1, 2 diol (MCPD) in bread from modern bakeries in Khartoum state, Sudan. Twenty five samples (25) of bread were collected from different modern bakeries of Khartoum, Khartoum Bahari and Omdurman, they include, round bread (13) samples and long bread (12) samples. The moisture content and oil content of samples were determined according to the AOAC method and the quantity of 3-MCPD by Gas Chromatography Mass Spectrometry (GC-MS) was investigated. 3-MCPD exposure for individual by body weight per day for adults and children was estimated. Moisture content in round bread ranged from 31.7% to 35.7%, and in long bread from 28.2% to 33.6%. Oil content in round bread ranged from 0.7955% to 0.9894% and oil content in long bread from 0.7684% to 1.005%. 3-MCPD was detected in round bread and long bread with a concentration of 0.15493-1.05873ppm to 0.11334-0.59644ppm, respectively, which were lower than that of European Commission Scientific Committee for Food Standardization. Exposure of adults and children to 3- MCPD upon consumption of bread is estimated to be (0.015446-0.031277µg/kg)-(0.002397-0.014118µg/kg)-(0.018039-0.036528µg/kg) and (0.002799-0.016488µg/kg) for adult and children, respectively. These exposure levels do not constitute a health risk.

Keywords

3-Monochloropropan-1, 2 diol,, round bread,, long bread,, Gas Chromatography Mass Spectrometry

Supporting Institution

Ministry of Higher Education & Scientific Research, Khartoum, Sudan with the research project number 130.

Project Number

130

Thanks

Authors thanks Ministry of Higher Education & Scientific Research, Khartoum, Sudan for their financial support

References

• AOAC 2000. Association of official Analytical Chemists. Official Methods for Analysis (15th.ed) Washington, D.C., USA.
• AOCS 2017 In: Firestone D (ed) Official methods and recommended practices of the American Oil Chemists’ Society. AOCS Press, Champaign
• Belkova, B., Chytilova, L., Kocourek, V., Slukova, M., Mastovska, K., Kyselka, J., Hajslova, J. 2020. Influence of dough composition on the formation of processing contaminants in yeast-leavened wheat toasted bread, Food Chemistry (2020), doi: https://doi.org/10.1016/j.foodchem.2020.127715.
• Breilling-Utzmann, C, M., Kobler, H., Herbolzheimer, D. 2003. 3-MCPD Occurrence in bread crust and various food groups as well as formation in long, Deut lebensm Rund Sch, 99: 280-285.
• Breitling-Utzmann, C. M., Hrenn H., Haase, N. U., Unbehend G. M. 2004. Influence of Dough Ingredients on 3-MCPD Formation in Long. Czech Journal of Food Science 22, 25-28.
• Crews A. C., Chiodini A., Granvogl, M., Hamle, T. C., Hrncirik, K., Kuhlmann, J., Lampen, A., Scholz G., Weishaar, R, Wenzl, T. 2013. Analytical Approaches for MCPD Esters and Glycidyl Esters in Food and Biological Samples: A Review and Future Perspectives, Food Additives Contaminants.30, 11–45.
• Divinova, V, Dolezal, M, Velisek, J. 2007. Free and bound 3-chloropropane-1, 2 diol in coffee surrogates and malts. Czech Journal of Food Science, 25: 39–47.
• Dolezal, I, M, Kertisova J,and Velisek J. 2009. Analysis of bread lipids for 3- MCPD esters, Czech Journal of Food Science 27: 417-419.
• Dolezal, M, Chaloupska, M, Divinova, V, Svejkovska B, Velisek J. 2005. Occurrence of 3- chloropropane-1, 2-diol and its esters in coffee. European Food Research & Technology, 221(3-4):221-225. Doi: 10-1007\500217-004-1118-8.
• Hamlet, C. G., Sadd, P. A., Gray, D. A. 2003. Influence of Composition, Moisture, pH and Temperature on the Formation and Decay Kinetics of Monochloro-propanediols in Wheat Flour Dough. European Food Research & Technology 216: 122–128.
• Hamlet, C.G., Sadd, P.A., Gray, D.A. 2004. Generation of monochloropropane -diols (MCPDs) in model dough systems. L. Leavened doughs. Journal of Agricultural and Food Chemistry.52, 2059-66.
• Hamlet, C. G., Jayaratne S. M., Matthews, W. (2002). 3-Monochloropropane-1, 2-diol (3-MCPD) in food ingredients from UK food producers and ingredient suppliers, Food Additives & Contaminants, 19:1, 15-21, DOI: 10.1080/02652030110072344
• Hwang M., Yoon E., Kim J. 2009. Toxicity value for 3-monochloropropane-1, 2-diol using a benchmark dose methodology. Regulatory Toxicology and Pharmacology 53(2):102-106. doi: 10.1016/j.yrtph.2008.12.005
• WHO/JEFCA. 2007. 3-chloro-1,2-propanediol(addendum). Safety evaluation of certain food additives and contaminants prepared by the sixty. Seventh meeting of the joint FHO\WHO, Expert, committee on food additives (JECFA), Geriava WHO food additives sci, 58:239-267.
• Karsulinova, L., Folprechtova, B., Dolezal, M., Dostalova, J., Velısek J. 2007. Analysis of the lipid fraction of coffee creamers, cream aerosols and bouillon cubes. Czech Journal of Food Science 25. 257-264
• Kertisova. 2009. Sited in Colin G, Lisa A, Jan V., Marek Z, Zuzana Z, and Colin C. 2010. The occurrence Of Fatty Acid Esters Of Chloropropandiols. A review prepared for The UK Food Standards Agency. Page 18-55.
• Muller, B.E., Booer, C.D., Slaiding, I. R. 2005. Formation of heat generated toxins during the processing of malt. Proc Congr Brew Conv, 30th 167-1-167\12.
• Stauf, A., Schneider, E., Heckel, F. (2020). 12 MCPD, 3 MCPD and fatty acid esters of 2 MCPD, 3 MCPD and glycidol in fine bakery wares. European Food Research and Technology, 246: 1945–1953.
• Svejkovska, B., Novotuy, O., Divinova, V. 2004. Esters of 3-chloropropane-1,2-diol (MCPD) in foodstuffs, Czech Journal of Food Science 22:190-196.
• Vannoort, R, W., Thomson, B, M. 2005. New Zealand total diet survey. Agricultural compound residues, selected contaminants and nutrients. Wellington: New Zealand food safety Authority.
• You, J, C, W., Kwong, K, D., Chung S. W. C. 2008. Dietary exposure to chloropropanols of secondary school students in Hong Kong. Food additive Contaminants part B. surveil, 1:93-99.