In vitro Propagation of Micromeria dolichodontha P.H. Davis via Axillary Shoot Proliferation

Year 2018, Issue: 14, 337 - 342, 31.12.2018

Yelda Emek

https://doi.org/10.31590/ejosat.492321

Cited By: 2

Abstract

The goal of this paper was to determine the protocol for in vitro axillary shoot propagation of Micromeria dolichodontha P.H. Davis, an endemic endangered
and medicinal plant. Plantlets obtained from in vitro germinated seeds
were used as explants for axillary shoot propagation. The plantlets were separated from their roots
and cultured on Murashige & Skoog (MS) media wihout and with different
types and concentrations of cytokinins (BA, KIN or TDZ). The maximum number of
shoots was formed on MS medium with 0.1 mgL^-1  benzyladenine (BA), and the highest seedling
length was formed on MS medium with 0.01 mgL^-1 BA. ½ MS medium
wihout and with different types and concentrations of auxin (IBA or IAA) were
used for rooting of shoots. The maximum root formation (75 %) were observed on
½ MS medium with 0.5 mgL^-1 IBA.

Keywords

Endemic, Micromeria dolichodontha, in vitro, axillary shoots

Micromeria dolichodontha P.H. Davis 'nın In vitro Aksiller Sürgün Yoluyla Çoğaltımı

Abstract

Bu
çalışmanın amacı endemik, nesli kritik tehlikede olan ve tıbbi Micromeria
dolichodontha P.H. Davis türünün
in vitro aksiller sürgün yoluyla çoğaltma protokolünü belirlemektir. In vitro şartlar altında tohumların
çimlendirilmesi ile elde edilen bitkicikler aksiller sürgün çoğaltımı için eksplant
olarak kullanılmışlardır. Bitkicikler köklerinden ayrılarak farklı tip ve
konsantrasyonda sitokinin (BA, KIN veya TDZ) içeren ve kontrol olarak herhangi
bir bitki büyüme düzenleyicisi içermeyen Murashige & Skoog (MS) besin ortamlarında
kültüre alınmışlardır. En yüksek sürgün sayısı 0.1 mgL^-1 benziladenine (BA)
içeren MS ortamında ve en uzun sürgünler 0.01 mgL^-1 BA
içeren MS ortamında oluşmuştur. Sürgünlerin köklendirilmesi için farklı tip ve
konsantrasyonda oksin (IBA veya IAA) içeren ve herhangi bir bitki büyüme
düzenleyicisi içermeyen ½ MS ortamı kullanılmıştır. En yüksek kök oluşumu (% 75)
0.5
mgL^-1 IBA içeren ½ MS ortamında gözlenmiştir.

Keywords

Endemik, Micromeria dolichodontha, in vitro, aksiller sürgünler

References

• Abou-Jawdah, Y., Wardan, R., Sobh, H., Salameh, A. 2004. Antifungal activities of extracts from selected Lebanese wild plants against plant pathogenic fungi. Phytopathologia Mediterranea 43, 377–386.
• Agostini, G., Echeverrigaray, S. 2006. Micropropagation of Cunila incisa Benth., a potential source of 1,8-cineole. Rev. Bras. Pl. Med., Botucatu 8, 186-189
• Ali-Shtayeh, MS., Al-Nuri M.A., Yaghmour, R.M.R., Faidi, YR.1997. Antimicrobial activity of Micromeria nervosa from the Palestinian Area. Journal of Ethnopharmacolog 58, 143–147.
• Arslan, N., Gürbüz, B., Gümüşçü, A., Sarıhan, E.O., İpek, A., Özcan, S., Mirici, S. 2002. Researches on cultivation of Sternbergia candida Mathew et. Baytop. Proceedings of 14th Meeting of Plant Originated Drugs Eskişehir, Turkey
• Aslan, I., Calmasur, O., Sahon, F., Caglar, O. 2005. Insecticidal effects of essential plant oils against Ephestia kuehniella (Zell.), Lasioderma serricorne (F.) and Sitophilus granarius (L.). Journal of Plant Diseases and Protection 112 (3), 257–267.
• Bassolino, L., Giacomelli, E., Giovanelli, S., Pistelli, L., Casetti, A., Damonte, G., Bisio, A., Ruffoni, B. 2015. Tissue culture and aromatic profile in Salvia dolomitica Codd. Plant Cell, Tissue and Organ Culture 121 (1), 83–95.
• Başer, K.H.C., Kırımer, N., Duman H. 1997. Composition of the essential oil of Micromeria dolichodontha P. H. Davis. Flavour and Fragrance Journal,, 12, 289-291
• Baytop, T. 1984. Therapy with Plants in Turkey, Istanbul University Publication (No. 2355) 520p, Istanbul, Turkey.Bezić, N., Dunkić, V., Vuko, E. 2013. Antiphytoviral Activity of Essential Oils of Some Lamiaceae Species and There Most Important Compounds on CMV and TMV. In: Méndez-Vilas, A. (ed.), Microbial pathogens and strategies for combating them: science, technology and education 2, 982-988.Formatex Research Center, Spain.
• Chen, U.C., Shiau, Y.J., Tsay, H.S., Hsia, C.N. 2005. Influence of cytokinin and ventilating container closure on shoot proliferation and hyperhydricity of in vitro Salvia miltiorriza culture. J. Taiwan Agric. Res. 54, 93-102.
• Davis, P. H. 1982. Flora of Turkey and the East Aegean Islands . Edinburgh University Press, . Edinburgh.Dudai, N., Poljakoff-Mayber, A., Mayer, A.M., Putievsky, E., Lerner, H.R. 1999. Essential oils as allelochemicals and their potential use as bioherbicides. Journal of Chemical Ecology 25(5), 1079-1089.
• Dudai, N., Chaimovitsh, D., Larkov, O., Fischer, R., Blaicher, Y., Mayer, A.M. 2009. Allelochemicals released by leaf residues of Micromeria fruticosa in soils, their uptake and metabolism by inhibited wheat seed. Plant Soil 314, 311–317.
• Duru, M.E., Öztürk, M., Ugur, A., Ceylan, O. 2004.The constituents of essential oil and in vitro antimicrobial activity of Micromeria cilicica from Turkey. Journal of Ethnopharmacology 94, 43–48.
• Echeverrigaray, S., Basso, R., Andrade, L.B. 2005. Micropropagation of Lavandula dentata from axillary buds of field-grown adult plants. Biol. Plant. 49, 439-442.
• Echeverrigaray, S. Carrer, R.S., Andrade, L.B. 2010. Micropropagation of Salvia guaranitica Benth. through axillary shoot proliferation. Brazilian Archives of Biology and Technology 53(4), 883-888.
• Ekim, T., Koyuncu, M., Vural, M., Duman, H., Aytaç, Z., Adıgüzel, N., 2000. Türkiye Bitkileri Kırmızı Kitabı (Red Data Book of Turkish Plants), Pteridophyta and Spermatophyta. Türkiye Tabiatını Koruma Derneği, Ankara.
• Elangomathavan, R., Prakash, S., Kathiravan, K., Seshadri, S., Ignacimuthu, S. 2003. High frequency in vitro propagation of Kidney Tea Plant. Plant Cell Tiss Org 72, 83-86.
• Erdağ, B., Emek, Y., Kurt, S. 2010. Clonal propagation of Dorystoechas hastata via axillary shoot proliferation. Turkish Journal of Botany 34, 233-240.
• Fracaro, F., Echeverrigaray, S. 2001. Micropropagation of Cunila galioides, a popular medicinal plant of south Brazil. Plant Cell, Tissue and Organ Culture 64, 1-4.
• Güllüce, M., Sökmen, M., Şahin, F., Sökmen, A., Adıgüzel, A., Özer, H. 2004. Biological activities of the essential oil and methanolic extract of Micromeria fruticosa (L.) Druce ssp. serpyllifolia (Bieb.) P.H. Davis plants from the eastern Anatolia region of Turkey. Journal of the Science of Food and Agriculture 84(7), 735–741.
• Güner, A., Özhatay, N., Ekim, T., Başer, C., 2000. “Flora of Turkey and the East Aegean Islands (Supplement 2)” Vol. 11. University Press, Edinburgh.
• Kadota, M., Niimi, Y. 2003. Effect of cytokinin types and their concentration on shoot proliferation and hyperhydricity in in vitro pear cultivar shoots. Plant Cell Tiss. Org. Cult. 72, 261-265.Lai-Keng, C., Leng, W. 2004. Plant regeneration from stem nodalsegments of Orthosiphon stamineus Benth., a medicinal plant with diuretic activity. In vitro Cell Dev Biol Plant 40,115-118.
• Murashige, T., Skoog, F. 1962. A revised medium for rapid growth and bioassays with tobacco tissue cultures. Plant Physiology 15, 473-497.
• Özcan, M. 1999. Antifungal effects of Micromeria myrtifolia Boiss. and Hohen in Boiss. and Prangos uechtritzii (Boiss.) Hawsskn decoctions. Acta Alimentaria 28, 355–360.Philips, C.G., Hubstenbenger, J.F. 1995. Micropropagation by proliferation of axillary buds In: Plant Cell, Tissue and Organ Culture Fundamental Methods. Gamborg, O. L. and Philips, G. C. (eds), 45-47, Germany.
• Rout, G.R., Samantaray, S., Das, P. 2000. In vitro manipulation and propagation of medicinal plants. Biotechnol Adv 18, 91–120
• Sarac, N., Ugur, A. 2007 Antimicrobial activites and usage in folkloric medicine of some Lamiaceae species growing in Muğla Turkey. EurAsian Journal of BioSciences 4, 28-37.
• Stojanovic, G., Palic, I. 2008. Antimicrobial and antioxidant activity of Micromeria Bentham Species. Current Pharmaceutical Design 14 (29), 3196-3202.
• Sunandakumari, C., Martin, K.P., Chithra, M., Sini, S., Madhusoodanan, P.V. 2004. Rapid axillary bud proliferation and ex vitro rooting of herbal spice, Mentha piperita L. Indian J Biotechnol 3, 108-112.
• Tabanca, N., Kirimer, N., Demirci, B., Demirci, F., Baser, K.H.C. 2001. Composition and antimicrobial activity of the essential oils of Micromeria cristata subsp. phrygia and the enantiomeric distribution of borneol. Journal of Agricultural and Food Chemistry 49, 4300-4303.
• Tošić, S., Nikolić, S., Jovanović, M., Zlatković, B., Stojičić, D.2015. Micropropagation of Micromeria juliana (L.) Benth.ex Rchb. (Lamiaceae). Biologica Nyssana 6(1), 17-23.Vladimir-Knežević, S., Blažeković, B., Bival Štefan, M., Alegro, A., Köszegy, T., Petrik, J. 2011. Antioxidant activities and polyphenolic contents of three selected Micromeria species from Croatia. Molecules 16, 1454-1470.
• Ziv, M., 1991. Vitrification: morphological and physiological disorders of in vitro plants. In: Debergh, P. C.; Zimmerman, R. H. (eds). Micropropagation– Technology and application. Dordrecht 45-69, Kluwer Academic Publish.
• Zuzarte, M.R., Dinis, A.M., Cavaleiro, C., Salgueiro, L. R., Canhoto, J. M. 2010. Trichomes, essential oils and in vitro propagation of Lavandula pedunculata (Lamiaceae). Industrial Crops and Products 32,580–587.