Marulda solgunluğa neden olan Fusarium oxysporum f. sp. lactucae etmenine karşı Metarhizium anisoplae’nın biyokontrol potansiyelinin belirlenmesi

Year 2022, Issue: 35, 335 - 340, 07.05.2022

Remzi Sağlık Nedim Altın

https://doi.org/10.31590/ejosat.1063983

Abstract

Bu çalışma entomopatojen fungus olan Metarhizium anisopliae’nın marulda solgunluk hastalığına neden olan Fusarium oxysporum f.sp. lactucae etmenine karşı antagonistik etkisini belirlemek amacıyla yürütülmüştür. Marul tarlalarından alınan hastalıklı bitkilerden Fusarium oxysporum f.sp. lactucae izolasyonu yapılmıştır. Daha önceki çalışmalardan elde edilen Metarhizium anisopliae izolatlarının antagonistik etkileri in vitro koşullarda ikili karşılaştırma yöntemine göre belirlenmiştir. İn vivo koşullarda ise saksı denemeleri yapılmıştır. Yapılan denemeler sonucunda in vitro koşullarda Fusarium oxysporum f.sp. lactucae etmenine karşı en iyi yüksek etkiyi % 60.44 ile YK33 izolatı göstermiştir. Fusarium oxysporum f.sp. lactucae patojeni ile Metarhizium anisopliae’nın YK33-c izolatı arasında ise 15.12 mm’lik engelleme bölgesi oluşmuştur. Saksı denemelerinde ise en az hastalık şiddeti % 30 ile YK33-c ve Yk43-b izolatlarında görülmüştür. Kontrole göre yapılan etki değerlendirmesinde her iki izolat hastalığa karşı % 65 etki göstermiştir. Elde edilen bu sonuçlar marulda solgunluğa neden olan Fusarium oxysporum f.sp. lactucae etmenine karşı entomopatojen fungus olan Metarhizium anisopliae’nın kullanılabileceğini ortaya koymuştur.

Keywords

Entomopatojen, Fusarium oxysporum f. sp. lactucae, Marul, Metarhizium anisoplae

Supporting Institution

Düzce Üniversitesi, Bilimsel Araştırma Projeleri Birimi

Project Number

Proje no: 2020.11.02.1075

Thanks

Yazarlar Metarhizium anisopliae izolatlarının temini için Prof. Dr. Salih KARABÖRKLÜ’ye teşekkür eder.

Determination of the biocontrol potential of Metarhizium anisoplae against Fusarium oxysporum f. sp. lactucae causing wilt in lettuce

Abstract

This study was carried out to determine the antagonistic effect of the entomopathogenic fungus Metarhizium anisopliae against Fusarium oxysporum f.sp. lactucae, which causes wilt disease in lettuce. Fusarium oxysporum f.sp. lactucae was isolated from diseased plants taken from lettuce fields. The antagonistic effects of Metarhizium anisopliae isolates obtained from previous studies were determined according to dual culture methods in vitro conditions. Pot experiments were carried out under in vivo conditions. As a result of the experiments, YK33 isolate showed the highest effect with 60.44% against Fusarium oxysporum f.sp. lactucae in vitro conditions. A 15 mm inhibition zone was formed between the pathogen Fusarium oxysporum f.sp. lactucae and the YK33-c isolate of Metarhizium anisopliae. In pot experiments, the least disease severity was determined with 30% in YK33-c and Yk43-b isolates. Both isolates showed 65% effect against disease in the effect evaluation compared to the control. These results revealed that the entomopathogenic fungus Metarhizium anisopliae can be used against Fusarium oxysporum f.sp. lactucae, which causes wilt in lettuce.

Keywords

Entomopathogen, Fusarium oxysporum f. sp. lactucae, Lettuce, Metarhizium anisoplae

Project Number

Proje no: 2020.11.02.1075

References

• Bucarei, L.B., Iglesias, A.F., González, M.G., Aguayo, G.S., Fernández, J.C., Castro, J.F. & Campos, J.O. (2020). Antifungal Activity of Beauveria bassiana Endophyte against Botrytis cinerea in Two Solanaceae Crops. Microorganisms, 8, 65.
• Claerbout, J., Venneman, S., Vandevelde, I., Decombel, A., Bleyaert, P., Volckaert, A., Neukermans, J. & Höfte, M. (2018). First Report of Fusarium oxysporum f. sp. lactucae Race 4 on Lettuce in Belgium . Plant Disease, 102, 1037.
• Dara, S.K. (2019). Non-Entomopathogenic Roles of Entomopathogenic Fungi in Promoting Plant Health and Growth. Insects, 10, 277.
• Dara, S.S.R., Dara, S.S. & Dara, S.K. (2018).Preliminary Report on the Potential of Beauveria bassiana and Metarhizium anisopliae s.l. in Antagonizing the Charcoal Rot Causing Fungus Macrophomina phaseolina in Strawberry. E-J. Entomol. Biol. Erişim:10.01.2022, <https://ucanr.edu/blogs/blogcore/postdetail.cfm?postnum=28274>
• Franco, F.R. (2018). A new approach for delivery of entomopathogenic fungi for plant protection against insect pests and plant diseases via maize seed coating. Lincon University, Thesis of Doctoral, Christchurch-New Zealand.
• Gilardi, G., Tinivella, F., Gullino, M.L. & Garibaldi, A. (2005). Seed dressing to control Fusarium oxysporum f. sp. Lactucae. Journal of Plant Diseases and Protection,112(3), 240-246.
• Gilardi, G., Franco Orteg, S., van Rijswick, P. C. J., Ortu, G., Gullino, M.L. & Garibaldi, A. (2017a). A new race of Fusarium oxysporum f. sp. lactucae of lettuce. Plant Pathology, 66, 677–688.
• Gilardi, G., C. Pons, B. Gard, S. Franco-Ortega & Gullino, M.L. (2017b). Presence of Fusarium Wilt, Incited by Fusarium oxysporum f.sp. lactucae, on Lettuce in France. Plant Disease, 101(6), 1053.
• Gilardi, G., Garibaldi, A. & Gullino, M.L. 2007. Effect of Antagonistic Fusarium spp. and of Different Commercial Biofungicide Formulations on Fusarium Wilt of Lettuce. Phytoparasitica, 35(5), 457-465.
• Gordon, T.R. & Koike. S.T. 2015. Management of Fusarium wilt of lettuce. Crop Protection, 73, 45-49.
• Gothandapani, S., Boopalakrishnan, G., Prabhakaran, N., Chethana, B. S., Aravindhan, M., Saravanakumar, M. & Ganeshan, G. (2015). Evaluation of entomopathogenic fungus against Alternaria porri (Ellis) causing purple blotch disease of onion. Archives of Phytopathology and Plant Protection, 48(2), 135-144.
• Hubbard JC. & Gerik JS. (1993). A new wilt disease of lettuce incited by Fusarium oxysporum f. sp. lactucum forma specialis nov. Plant Disease, 77, 750–754.
• Karabörklü S., Altın, N. & Keskin, Y. (2019). Native Entomopathogenic fungi isolated from Duzce, Turkey and their virulence on the mealworm beetle Tenebrio molitor L. (Coleoptera: Tenebrionidae). Philippines Agricultural Scientist, 102(1), 82-89.
• Matuo T. & Motohashi S. (1967). On Fusarium oxysporum f. sp. lactucae n.f. causing root rot of lettuce. Transactions of the Mycological Society of Japan, 8, 13–5.
• Meyling, N.V., Arthur, S., Pedersen, K.E., Dhakal, S., Cedergreen, N. & Fredensborg, B.L., (2018). Implications of sequence and timing of exposure for synergy between the pyrethroid insecticide alpha‐cypermethrin and the entomopathogenic fungus Beauveria bassiana. Pest Management Science, 74, 2488–2495.
• Nair, M.N., Simon, S. & Lal, A.A. (2021). Effect of Metarhizium anisopliae on Rhizoctonia solani and Meloidogyne graminicola in Rice Seedlings. Int.J.Curr.Microbiol.App.Sci., 10(03), 895-903.
• Hao, Q., Albaghdady, D.M.D., Yannong Xiao, Y., Xueqiong Xiao, X., Chenmi Mo, C., Tian, T. & Wang, G. (2021). Endophytic Metarhizium anisopliae is a potential biocontrol agent against wheat Fusarium head blight caused by Fusarium graminearum. Journal of Plant Pathology,103, 875-885.
• Paugh, K.R. & Gordon, T.R. (2020). The population of Fusarium oxysporum f. Sp. lactucae in California and Arizona. Plant disease, 104,1811-1816.
• Rath, A.C. (2000). The use of entomopathogenic fungi for control of termites. Biocontrol Science and Technology, 10, 563-581.
• Ravindran, K., Chitra, S.,Wilson, A. & Sivaramakrishnan, S. (2014). “Evaluation of antifungal activity of Metarhizium anisopliae against plant phytopathogenic fungi,” in Microbial Diversity and Biotechnology in Food Security (New Delhi: Springer), 251–255.
• Roberti, R., Ghisellini, L. & Innocenti, G. (1993). Biological control of blackleg of beet (Phoma betae) by Metarhizium anisopliae. Journal of Plant Diseases and Protection, 100(2), 203-210.
• Sarven, M.S., Hao, Q., Deng, J., Fang Yang, F., Wang, G., Xiao, Y. & Xiao, X. (2020). Biological Control of Tomato Gray Mold Caused by Botrytis Cinerea with the Entomopathogenic Fungus Metarhizium anisopliae. Pathogens, 9(3),1-17.
• Sasan, R.K. (2012). The analysis of Metarhizium robertsii potential as endophytic plant root coloniser, plant growth enhancer and antagonist to bean root pathogen Fusarium solani f. sp. phaseolis. Brock Universty, Thesis of MSc, St. Catharines, Ontario.
• Taylor, A., Jackson, A.C. & Clarkson, J.P. (2019). First Report of Fusarium oxysporum f. sp. lactucae Race 4 Causing Lettuce Wilt in England and Ireland. Plant Disease, 103(5), 1033-1033.