Determination of TNF-beta Marker in Intrauterine E. coli Induced Endometritis Model in Rats

Year 2021, Volume: 3 Issue: 1, 16 - 20, 30.03.2021

Mustafa Makav , Mushap Kuru

https://doi.org/10.51262/ejtox.893215

Cited By: 1

Abstract

Objectives: This study aims to investigate the importance of tumor necrosis factor (TNF)-beta marker in the endometritis model created by E. coli.

Materials and Methods: Rats were divided into control and endometritis groups with 7 rats in each group in the study. All animals were fed as ad libitum. No application was made to rats in the control group. Progesterone (P4) was administered subcutaneously to rats in the experimental group at a daily dose of 16 mg/kg between the 0th and 4th days of the study. E. coli was administered intrauterinely at 25 μl and 1 × 105 CFU/rat on the 3rd day. Blood samples were taken at the end of the study after the lives of all animals were terminated in accordance with ethical rules. Hemogram analysis was performed for white blood cell (WBC) levels from the blood samples taken. The TNF-beta analysis was performed with a commercial kit by separating serum from blood samples.

Results: WBC (p=0.0004) and TNF-beta (p=0.027) were statistically higher in the endometritis group compared to the control group.

Conclusion: Consequently, increased TNF-beta in endometritis may be a diagnostic marker for inflammations in the genital tract. However, its effectiveness may be better demonstrated by conducting comprehensive studies with the relevant marker.

Keywords

TNF-beta, WBC, Endometritis, Rat

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