Genetic Variation in Drought Linked Morpho-physiological Characters and Microsatellite DNA Loci in Rice (Oryza sativa L.)

Year 2017, Volume: 3 Issue: 1, 25 - 40, 31.01.2017

Pankaj Bhatıa

Abstract

Evaluation for drought tolerance and SSR (microsatellite) markers based molecular polymorphism were investigated
in F6
plant population raised via single seed descent method from a cross between drought tolerant japonica rice
variety Azucena and drought sensitive premium traditional Basmati rice variety Taraori Basmati HBC 19. A total
of 50 F6
plants were evaluated individually for drought tolerance on 1-9 scale on the basis of agronomic characteristics,
root and shoot traits, relative water content and visual observations; the average score ranged between 1to
8.3. Fourteen plants each in the category of drought tolerant and drought sensitive were selected from F6
population
for SSR marker analysis using 30 SSR markers covering all the chromosomes. The 28 Azucena × HBC19 F6
plants
had an allele from either of the two parental lines (homozygous condition) or alleles from both the parental rice varieties
(heterozygous condition). Frequency of HBC19 specific alleles was higher in comparison to Azucena in selected
drought tolerant and drought sensitive Azucena x HBC19 F6
plants, which may be indicative of segregation distortion.
At ten SSR loci new/recombinant alleles were obtained which indicate the active recombination between genomes of
two rice varieties. Cluster tree analysis and principal component analysis demonstrate hig

Keywords

Genetic diversity; drought stress; microsatellite; Oryza sativa; root traits;, recombinant inbred lines

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