Floral and Molecular Characterization of Gamma Rays Induced Mutants in Oil-Bearing Rose (Rosa damascena Mill.)

Year 2021, Volume: 7 Issue: 1, 21 - 33, 01.01.2021

Hasan Baydar , Ufuk Çelikkol Akçay , Soner Kazaz , Nilgün Göktürk Baydar

Abstract

This research was aimed to create the genetic variations for the selection of oil-bearing rose genotypes with the desirable
floral features. For this reason, gamma rays at doses of 0, 100 and 200 Gy of radioactive Cobalt-60 were applied to oilbearing
rose seeds to create genetic variations. Finally, the floral and molecular characterization of oil-bearing rose plants
derived from non-irradiated and irradiated seeds were carried out. A total of 48 genotypes including 17 from control
(0 Gy), 18 from M100 (100 Gy) and 12 from M200 (200 Gy) together with the parental species R. damascena were used
as genetic materials. Genetic analysis was performed by using 20 SSR primer pairs. After the electrophoresis, the bands
were displayed in TIF format with the aid of the Biolab UV Tech gel imaging system. The genotypes and mutants grown
from irradiated and non-irradiated seeds were significantly different with flower colours from white to dark pink and petal
numbers from 5 to 100. These results also showed that the seeds in the open-pollinated flowers of oil-bearing rose had
mostly heterozygous allele genes governing the floral traits. The efficacy of the SSR primers used to identify mutations
was different. RA003a and RA034a primers were found to be more effective in mutation screening in oil-bearing rose
genome. It was understood that the major effects of the gamma-ray irradiation were on large-scale chromosomal breaks
or deficiencies. In conclusion, oil-bearing rose seeds with or without gamma-ray irradiation would be a huge selection
source to breed novel varieties.

Keywords

Rosa damascena Mill., mutation breeding, gamma-ray, SSRs

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