Assessment Through Multilocation Trial for Unique Germplasm of Hibiscus sabdariffa L. with Special Reference to Flower and Fruit Colour

Year 2024, Volume: 10 Issue: 2, 119 - 126, 31.07.2024

Mohan Lal Twahira Begum Tanmita Gupta

Abstract

Hibiscus sabdariffa L. commonly referred to as “Roselle” and “red sorrel” is a globally known annual herbaceous species
of Malvaceae family holds utmost significance owing to its medicinal properties namely antioxidant, antimicrobial, and
also for its proven utility as a natural colorant. The dark-red colored anthocyanin rich calyx has multi-purpose utilities
including food, beverages, and medicines. The plant species shows its significant and promising potential in avenues of
natural red colorants particularly water-soluble ones. Highly adaptive to varying soil conditions and humidity, the North
Eastern states of India serve as a perfect cultivation house for this exquisite plant. In the present study 32 germplasm were
planted for subsequent year Kharif 2021 and Kharif 2022 in randomized block design with three replicates. From the two
years’ evaluation 5 identified lines were subjected to multi-locational trials (MLT) along with one check variety named
as local variety comprising of four different locations of NE India during Kharif 2023. Diverse climatic conditions of this
region are responsible for the formation of new genotypes with varying chemical compositions. For this purpose, the MLT
was performed at four different locations of North East India to confirm their stability performance. The main objective
was to carry out a morphological and qualitative data analysis of H. sabdariffa. Plant height, colour of flower, colour of
fruit, number of fruits per plant, average fruit weight and stem colour were evaluated. The qualitative and quantitative
results demonstrate the identified lines were superior to that of the local (check) variety.

Keywords

Calyx, fruit size, flower colour, multilocation trial, roselle

References

• Ahmed K and Hudson F, (1979). The fatty acid composition of Hibiscus sabdariffa seed oil. J Sci Food Agric 33:1305-9.
• Ali BH, Wabel NA and Blunden G, (2005). Phytochemical, pharmacological and toxicological aspects of Hibiscus sabdariffa L.: a review. Phytother Res An International Journal Devoted to Pharmacological and Toxicological Evaluation of Natural Product Derivatives 19(5):369-75.
• Ali MK, Ashraf A, Biswas NN, Kamakar UK and Afroz S, (2011). Antinociceptive, anti-inflammatory and antidiarrheal activities of ethanolic calyx extract of Hibiscus sabdariffa Linn. (Malvaceae) in mice. J Integr Med 9(6):626-631.
• Ali SAE, Mohamed AH and Mohammed GEE, (2014). Fatty acid composition, anti-inflammatory and analgesic activities of Hibiscus sabdariffa Linn. Seeds. J Adv Vet Anim Res1 (2):50-57.
• Amlashi HA, Madani H, Sonboli A, Khaghani S and Ramezani M, (2020). Volatile composition of the leaves and calyces essential oil of roselle (Hibiscus sabdariffa L.) from Iran. J Essent Oil- Bear Plants 23(4):743-755.
• Bako IG, Mabrouk MA and Abubakar A, (2009). Antioxidant effect of ethanolic seed extract of Hibiscus sabdariffa Linn. (Malvaceae) alleviate the toxicity induced by chronic administration of sodium nitrate on some haematological parameters in wistars rats. Adv J Food Sci Technol 1(1):39-42.
• Betiku and Adepoju, (2013). Sorrel (Hibiscus sabdariffa) Seed oil extraction optimization and quality characterization. Am Chem Sci J3(4):449- 458
• Camelo-Méndez GA, Ragazzo-Sánchez JA, Jiménez- Aparicio AR, Vanegas-Espinoza PE and Paredes-López O, (2013). Comparative study of anthocyanin and volatile compounds content of four varieties of Mexican roselle (Hibiscus sabdariffa L.) by multivariable analysis. Plant Foods Hum Nutr 68(3):229-234.
• Carvajal-Zarrabal O, Barradas-DermitzDM,Orta- Flores Z, Hayward-Jones PM, Nolasco-Hipólito C and Aguilar- Uscanga MG,(2012). Hibiscus sabdariffa L., roselle calyx, from ethnobotany to pharmacology. J Exp Pharmacol 4:25-29.
• Cisse M, Valliant F, Acosta O, Dhuique-Mayer C and Dornier M, (2009). Thermal de- gradation kinetics of anthocyanins from blood orange, blackberry, and roselle using arrhenius, eyring, and ball models. J Agric Food Chem 57:6285-6291.
• Daudu O, Yusuf A, Falusi O, Dangana MC, Abubakar A and Yahaya SA, (2015). Collection and evaluation of roselle (Hibiscus sabdariffa L.) germplasm in Nigeria. Afr J Food Sci 9(3):92-96.
• Duke JA and DuCellier JL, (1993). CRC Handbook of Alternative Cash Crops. 1st ed. CRC Press Publisher.
• Gautam RD, (2004). Sorrel- a lesser known source of medicinal soft drink and food in India. Nat Prod Radiance 3(5):338-341. https://www.drugs.com/ npc/hibiscus.html
• Idris MHM, Budin SB, Osman M and Mohamed J, (2012). Protective role of Hibiscus sabdariffa calyx extract against streptozotocin induced sperm damage in diabetic rats. EXCLI J 11:659-669.
• Ilodibia CV, AchebeUA, ArubaluezeCU and IbehE, (2019). A comparative study on morphology, mineral and vitamin compositions of two variants of Hibiscus sabdariffa (L.) AAES 4(1):50-56 https://doi.org/10.26832/24566632.2019.040108
• Inikpi E, Lawal AO, Ogunmoye AR and Ogunwande AI, (2014). Volatile composition of the floral essential oil of Hibiscus sabdariffa L. from Nigeria. Am J Essent Oil Nat Prod 2(2):04-07.
• Jones PJ, Raeini-Sarjaz M, Ntanios FY, Vanstone CA, Feng JY and Parsons WE, (2000). Modulation of plasma lipid levels and cholesterol kinetics by phytosterol versus phytostanol esters. J Lipid Res 41:697-705
• Khaghani S, Farideh R, Mohsen MY, Malihe P, Ahmad S and Parvin P, (2011). Selective cytotoxicity and apoptogenic activity of Hibiscus sabdariffa aqueous extract against MCF-7 Human breast cancer cell line. J Cancer Ther 2:394-400.
• Kolawole OT, Akiibinu MO and Akanji MA, (2014). Assessment of the effect of aqueous extract of calyx of Hibiscus sabdariffa on some biochemical indices of renal function in rats. Int J PharmSci 4(3):587-590.
• Kristen LH, Kellie PB, Svetlana Z, Michael PD and Stewart JRCN, (2014). Antimicrobial activity of Hibiscus sabdariffa aqueous extracts against Escherichia coli O157:H7 and Staphylococcus aureus in a microbiological medium and milk of various fat concentrations. J Food Prot 77(2):262-268.
• Mahadevan N and Kamboj P, (2009). Hibiscus sabdariffa Linn.-An Overview. NOPR 8(1):77- 83, 0972-592X
• Mckay DL, Chen CYO, Saltzman E and Blumberg JB, (2009). Hibiscus sabdariffa L. tea (Tisane) lowers blood pressure in prehypertensive and mild hypertensive adults1- 4. J Nut 109:298-303.
• Mungole A and Chaturvedi A, (2011). Hibiscus sabdariffa L. a rich source of secondary metabolites. Int J Pharm Sci Rev Res 6(1):83-87. Nyam KL, Tan CP, Lai OM, Long K and Che Man YB, (2009). Physicochemical properties and bioactive compounds of selected seed oils. LWT - Food SciTechnol 42(8):1396-1400.
• Ologundudu A, Ologundudu AO, Ololade IA and Obi FO, (2009). Effect of Hibiscus sabdariffa anthocyanins on 2, 4- dinitrophenylhydrazineinduced hematotoxicity in rabbits. Afr J Biochem Res 3(4):140-144.
• Orwa C, Mutua A, Kindt R, Jamnadass R and Simons A, (2014). Agroforestree Database: A tree reference and selection guide version 4. Ramirez-Rodrigues MM, Plaza ML, Azeredo A, Balaban MO and Marshall MR, (2012). Phytochemical, sensory attributes and aroma stability of dense phase carbon dioxide processed Hibiscus sabdariffa beverage during storage, Food Chem 134:1425-1431.
• Rao PU, (1996). Nutrient composition and biological evaluation of mesta (Hibiscus sabdariffa) seeds. Plant Foods Hum Nutr 49(1):27-34.
• Richardson ML and Arlotta CG, (2021). Differential yield and nutrients of Hibiscus sabdariffa L. genotypes when grown in urban production systems. Sci Hortic., 288, 110349.
• Sandeep G, Raghuveer I, Prabodh CS, Suresh T, Atin K and Aakash D, (2010). Hypolipidemic effect of ethanolic extract from the leaves of Hibiscus sabdariffa L. in hyperlipidemic rats. Acta Pol Pharm Drug Res 67(2):179-184.
• Sanders M, Ayeni AO and Simon JE, (2020). Comparison of yield and nutrition of roselle (Hibiscus sabdariffa) genotypes in central New Jersey. JMAP 9(4):242-252.
• Stevels JMC, (1990). Legumes traditionnels du Cameron, une etude Agrobotanique. Wageningen, Agricultural University, Wageningen.
• Ubani CS, Joshua PE and Oraeki AN, (2010). Influence of aqueous extract of Hibiscus sabdariffa calyces on lipid profile of phenobarbitone induces wistar albino rats. J Pharm Res 3(2):319-24.
• Wang ML, Morris B, Tonnis B, Davis J and Pederson GA, (2012). Assessment of oil content and fatty acid composition variability in two economically important Hibiscus species. J Agric Food Chem 60(26):6620-6626.
• Wisetmuen E, Pannangpetch P, Kongyingyoes B, Kukongviriyapan U and Itharat A, (2008). Antidiabetic effect of ethanolic extract of Roselle (Hibiscus sabdariffa) in chronic streptozotocininduced diabetic rats. Thai J Pharmacol 29(1):69- 73.
• Zhang YN and Wang ZZ, (2007). Essential oil composition of Hibiscus sabdariffa from Yunnan, China. Chem NatCompd 43(6):714-715