Effect of plant phenolic compounds on the hemocyte concentration and antioxidant enzyme activity in Hyphantria cunea (Drury, 1773) (Lepidoptera: Arctiidae) larvae infected by Hyphantria cunea granulovirus

Year 2022, Volume: 46 Issue: 1, 37 - 49, 01.03.2022

Oğuzhan Yanar , Elif Topkara , Sevcan Mercan , İsmail Demir , Zeynep Bayramoğlu

https://doi.org/10.16970/entoted.1000271

Cited By: 2

Abstract

The aim of this study was to determine the effects of phenolic substances in different plants (mulberry, apple, walnut and plum) on hemocyte counts and antioxidant enzyme activities of Hyphantria cunea larvae infected with Hyphantria cunea granulovirus (HycuGV) and uninfected. In the study, the phenolic contents of plant materials were firstly determined. Then, the effects of these ingredients on hemocyte counts and antioxidant enzyme activities of infected and uninfected larvae were determined. As a result of the study, the hemocyte counts of all groups increased with the infection was determined. In enzyme activity, the phenoloxidase (PO) activities of larvae fed on apple leaves and plum leaves containing rosmarinic acid and protocatechuic acid increased with infection was determined. The amounts of malondialdehyde (MDA) decreased in all groups as a result of viral infection. While the highest superoxide dismutase (SOD) and catalase (CAT) activities among both infected and uninfected larvae were in the plum groups with the highest amount of chlorogenic acid, the lowest glutathione peroxidase (GSH-Px) activities were also in these groups. All this showed that different phenolic contents of host plants affected the hemocyte counts and antioxidant enzyme activities of H. cunea larvae.

Keywords

Antioxidant activity, granulovirus, hemocyte, Hyphantria cunea, phenolic compounds

Supporting Institution

Ondokuz Mayıs University Research Foundation Turkey, Grant Project

Project Number

PYO.FEN. 1904.18.002

Thanks

We thank Dr. Ardahan Eski from Bilecik Seyh Edebali University for his contributions. This study was supported by the Ondokuz Mayıs University Research Foundation (PYO.FEN. 1904.18.002).

Hyphantria cunea granulovirus tarafından enfekte edilen Hyphantria cunea (Drury,1773) (Lepidoptera: Arctiidae) larvalarının hemosit konsantrasyonu ve antioksidan enzim aktivitelerine bitki fenolik bileşiklerinin etkisi

Abstract

Bu çalışmanın amacı, dört bitkide (elma, dut, erik ve ceviz) bulunan fenolik maddelerin Hyphantria cunea granulovirus ile enfekte ve enfekte olmamış Hyphantria cunea (Drury, 1773) (Lepidoptera: Arctiidae) larvalarının hemosit konsantrasyonları ve antioksidan enzim aktivitesine olan etkilerini belirlemekti. Bu çalışmada kullanılan bitkiler Bafra, Samsun, Türkiye’de 2019 yılında toplandı. Bu bitkilerin yapraklarının fenolik konsantrasyonları belirlendi. Daha sonra, bu fenoliğin enfekte ve enfekte olmayan larvaların hemosit konsantrasyonlarına ve antioksidan enzim aktivitesine olan etkisi belirlendi. Tüm grupların hemosit konsantrasyonlarının virüs enfeksiyonu ile arttığı belirlendi. Viral enfeksiyon sonucu tüm gruplarda malondialdehit konsantrasyonu azaldı. Hem enfekte hem de enfekte olmayan larvalar arasında en yüksek süperoksit dismutaz ve katalaz aktiviteleri klorojenik asit konsantrasyonunun en yüksek olduğu erik gruplarında iken, en düşük glutatyon peroksidaz aktivitesi de bu gruplardaydı. Bütün bunlar, konukçu bitkilerin farklı fenolik konsantrasyonlarının H. cunea larvalarının hemosit konsantrasyonlarını ve antioksidan enzim aktivitesini etkilediğini göstermiştir.

Keywords

Antioksidan aktivite, granulovirüs, hemosit, Hyphantria cunea, fenolik bileşikler

Project Number

PYO.FEN. 1904.18.002

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