Research Article
Molecular identification and biodiversity of wireworm species, Agriotes spp. Eschscholtz, 1829 (Coleoptera: Elateridae) in major potato cultivated areas of Türkiye
Abstract
Wireworms, Agriotes spp. Eschscholtz, 1829 (Coleoptera: Elateridae) are among the most harmful soil-borne insect pests and significantly reduce potato yields under heavy infestations. The presence of wireworm species on potatoes in potato growing areas is not fully known in the provinces of Türkiye. Therefore, this research aimed to identify wireworms molecularly and evaluate their biodiversity in potato growing ecosystems. Here, the first extensive field survey was carried out in Türkiye's principal potato-growing regions in 2019 and 2020 (Afyon, Bolu, İzmir, Kayseri, Konya, Niğde, and Sivas). Species identification of wireworms was performed using DNA barcoding approach based on the fragment of mitochondrial cytochrome c oxidase subunit I (COI). Samples were collected from 400 potato fields, and 510 larval specimens were obtained. The presence of wireworms was confirmed for Afyon, Bolu, Kayseri, Konya, and Sivas provinces, with an average prevalence of 13.5%, while no positive samples were recovered from Niğde and İzmir. Agriotes sputator (L.,1758) (Coleoptera: Elateridae) was the most prevalent species in surveyed areas. The Shannon index of wireworm species was found to be as 0.59, which implies a low degree of biodiversity of wireworms in potato fields.
Keywords
Supporting Institution
The Scientific and Technological Council of Türkiye (TUBITAK/ TOVAG)
Project Number
Grant Project No: 115R025
Thanks
The authors thank to the Scientific and Technological Council of Türkiye (TUBITAK/ TOVAG) for the financial support.
References
- Andrews, K. R., A. Gerritsen, A. Rashed, D. W. Crowder, S. I. Rondon, W. G. van Herk & S. S. Hunter, 2020. Wireworm (Coleoptera: Elateridae) genomic analysis reveals putative cryptic species, population structure and adaptation to pest control. Communications Biology, 3 (1): 1-13.
- Benefer, C. M., W. G. Van Herk, J. S. Ellis, R. P. Blackshaw, R. S. Vernon & M. E. Knight, 2013. The molecular identification and genetic diversity of economically important wireworm species (Coleoptera: Elateridae) in Canada. Journal of Pest Science, 86 (1): 19-27.
- Cate, P. C., 2007. Family Elateridae Leach, 1815 (Cebrioninae, Lissominae, Subprotelaterinae). Catalogue of Palaearctic Coleoptera, 4: 94-207.
- Cherry, R., 2007. Seasonal population dynamics of wireworms (Coleoptera: Elateridae) in Florida sugarcane fields. Florida Entomologist, 90 (3): 426-430.
- Etzler, F. E., K. W. Wanner, A. Morales-Rodriguez & M. A. Ivie, 2014. DNA barcoding to improve the species-level management of wireworms (Coleoptera: Elateridae). Journal of Economic Entomology, 107 (4): 1476-1485.
- Folmer, O., W. R. Hoeh, M. B. Black & R. C. Vrijenhoek, 1994. Conserved primers for PCR amplification of mitochondrial DNA from different invertebrate phyla. Molecular Marine Biology and Biotechnology, 3 (5): 294-299.
- Furlan, L. & M. Tóth, 2007. Occurrence of click beetle pest (Coleoptera, Elateridae) in Europe as detected by pheromone traps: Survey results of 1998-2006. IOBC/WPRS Bulletin, 30 (7): 1-19.
- Furlan, L., 1998. The biology of Agriotes ustulatus Schäller (Col., Elateridae). II. Larval development, pupation, whole cycle description and practical implications. Journal of Applied Entomology, 122 (1‐5): 71-78.
- Furlan, L., 2014. IPM thresholds for Agriotes wireworm species in maize in Southern Europe. Journal of Pest Science, 87 (4): 609-617.
- Furlan, L., I. Benvegnù, M. F. Bilò, J. Lehmhus & E. Ruzzier, 2021. Species identification of wireworms (Agriotes spp.; Coleoptera: Elateridae) of agricultural importance in Europe: A new "Horizontal Identification Table". Insects, 12 (6): 1-12.
- Gülperçin, N. & S. Tezcan, 2021. An Evaluation on Elateridae (Insecta: Coleoptera) Fauna of Eastern Anatolia Region. International Journal of Food Agriculture and Animal Sciences, 1 (1): 31-36.
- Kabalak, M. & O. Sert, 2011. Faunistic composition, ecological properties and zoogeographical composition of the family Elateridae (Coleoptera) of the Central Anatolian Region of Türkiye. Journal of Insect Science, 11 (1): 1-36.
- Kabalak, M. & O. Sert, 2021. Eleteridae (Coleoptera) of the Eastern Black Sea region of Türkiye: Fauna, some ecological evaluations, and zoogeographical composition. Transactions of the American Entomological Society, 147 (1): 157-191.
- Kabalak, M., 2018. Research on the family Elateridae (Coleoptera) of Türkiye: New distributional data, female descriptions and distributional evaluation. Transactions of the American Entomological Society, 144 (1): 143-166.
- Kabalak, M., O. Sert & D. Kondo, 2013. Faunistic composition, ecological properties, and zoogeographical composition of the Elateridae (Coleoptera) family in the Western Black Sea region of Türkiye. Journal of Insect Science, 13 (1): 1-21.
- Keiser, A., M. Häberli & P. Stamp, 2012. Quality deficiencies on potato (Solanum tuberosum L.) tubers caused by Rhizoctonia solani, wireworms (Agriotes ssp.) and slugs (Deroceras reticulatum, Arion hortensis) in different farming systems. Field Crops Research, 128: 147-155.
- Kroschel, J., N. Mujica, J. Okonya & A. Alyokhin, 2020. “Insect Pests Affecting Potatoes in Tropical, Subtropical, and Temperate Regions, 80-123”. In: The Potato Crop: Its Agricultural, Nutritional and Social Contribution to Humankind (Eds. H. Campos & O. Ortiz). Springer Nature, New York, 506 pp.
- Kuhar, T. P. & J. M. Alvarez, 2008. Timing of injury and efficacy of soil-applied insecticides against wireworms on potato in Virginia. Crop Protection, 27 (3-5): 792-798.
- Kumar, S., G. Stecher, M. Li, C. Knyaz & K. Tamura, 2018. MEGA X: molecular evolutionary genetics analysis across computing platforms. Molecular Biology and Evolution, 35 (6): 1547-1549.
- Lindroth, E. & T. L. Clark, 2009. Phylogenetic analysis of an economically important species complex of wireworms (Coleoptera: Elateridae) in the Midwest. Journal of Economic Entomology, 102 (2): 743-749.
- Lumeran, B. T., 2019. “Assemblage of Gastropods in the Rocky Intertidal Zone of Asry Beach, Kingdom of Bahrain, 125-147”. In: Invertebrates-Ecophysiology and Management (Eds. S. Ray, G. Diarte-Plata & R. Escamilla-Montes). Intech Open, London, 165 pp.
- Milosavljević, I., A. D. Esser & D. W. Crowder, 2017. Seasonal population dynamics of wireworms in wheat crops in the Pacific Northwestern United States. Journal of Pest Science, 90 (1): 77-86.
- Parker, W. E. & J. J. Howard, 2001. The biology and management of wireworms (Agriotes spp.) on potato with particular reference to the UK. Agricultural and Forest Entomology, 3 (2): 85-98.
- Poggi, S., R. Le Cointe, J. Lehmhus, M. Plantegenest & L. Furlan, 2021. Alternative strategies for controlling wireworms in field crops: A review. Agriculture, 11 (5): 1-30.
- Ritter, C. & E. Richter, 2013. Control methods and monitoring of Agriotes wireworms (Coleoptera: Elateridae). Journal of Plant Diseases and Protection, 120 (1): 4-15.
- Shannon, C. E., 1948. A mathematical theory of communication. The Bell System Technical Journal, 27 (3): 379-423.
- Staudacher, K., N. Schallhart, P. Pitterl, C. Wallinger, N. Brunner, M. Landl & M. Traugott, 2013. Occurrence of Agriotes wireworms in Austrian agricultural land. Journal of Pest Science, 86 (1): 33-39.
- Susurluk, I. A., 2008. Effects of various agricultural practices on persistence of the inundative applied entomopathogenic nematodes, Heterorhabditis bacteriophora and Steinernema feltiae in the field. Russian Journal of Nematology, 16 (1): 23-32.
- Tamura, K., M. Nei & S. Kumar, 2004. Prospects for inferring very large phylogenies by using the neighbor-joining method. Proceedings of the National Academy of Sciences, 101 (30): 11030-11035.
- Traugott, M., C. M. Benefer, R. P. Blackshaw, W. G. van Herk & R. S. Vernon, 2015. Biology, ecology, and control of elaterid beetles in agricultural land. Annual Review of Entomology, 60: 313-334.
- Zhang, S., Y. Liu, J. Shu, W. Zhang, Y. Zhang & H. Wang, 2019. DNA barcoding identification and genetic diversity of bamboo shoot wireworms (Coleoptera: Elateridae) in South China. Journal of Asia-Pacific Entomology, 22 (1): 140-150.
Tel kurdu türlerinin, Agriotes spp. Eschscholtz, 1829 (Coleoptera: Elateridae) Türkiye'nin başlıca patates ekim alanlarındaki biyoçeşitliliği ve moleküler tanımlanması
Abstract
Tel kurtları, Agriotes spp. Eschscholtz, 1829 (Coleoptera: Elateridae), en önemli toprak kaynaklı zararlı böcekler arasında yer almakta ve yoğun istilalarda patates üretiminde ciddi verim kayıplarına neden olmaktadır. Türkiye'de patates yetiştirilen alanlarda zararlı tel kurdu türlerinin varlığı tam olarak bilinmemektedir. Bu nedenle bu çalışma, patates yetiştiriciliği yapılan ekosistemlerde tel kurdu türlerinin moleküler olarak tanılanmasını ve biyoçeşitliliğini belirlemeyi amaçlamıştır. Bu çalışmada, 2019 ve 2020 yıllarında Türkiye'nin başlıca patates yetiştirilen bölgelerinde (Afyon, Bolu, İzmir, Kayseri, Konya, Niğde ve Sivas) kapsamlı ilk saha araştırması yapılmıştır. Tel kurtlarının tür tanılaması, mitokondriyal sitokrom c oksidaz Alt Ünite I (COI) geni dizisine dayalı DNA barkodlaması kullanılarak yapılmıştır. Örnekler, 400 adet patates tarlasından toplanmış ve 510 adet larva örneği elde edilmiştir. Afyon, Bolu, Kayseri, Konya ve Sivas illerinde tel kurtlarının yaygınlığı ortalama %13,5 olarak belirlenirken, Niğde ve İzmir illerinden pozitif örnek elde edilmemiştir. Agriotes sputator (L., 1758) (Coleoptera: Elateridae) türünün örnekleme yapılan alanlarda en yaygın tür olduğu belirlenmiştir. Tel kurdu türlerinin Shannon indeksi 0.59 olarak bulunmuş olup, patates tarlalarında tel kurtlarının biyolojik çeşitliliğinin düşük olduğu belirlenmiştir.
Project Number
Grant Project No: 115R025
References
- Andrews, K. R., A. Gerritsen, A. Rashed, D. W. Crowder, S. I. Rondon, W. G. van Herk & S. S. Hunter, 2020. Wireworm (Coleoptera: Elateridae) genomic analysis reveals putative cryptic species, population structure and adaptation to pest control. Communications Biology, 3 (1): 1-13.
- Benefer, C. M., W. G. Van Herk, J. S. Ellis, R. P. Blackshaw, R. S. Vernon & M. E. Knight, 2013. The molecular identification and genetic diversity of economically important wireworm species (Coleoptera: Elateridae) in Canada. Journal of Pest Science, 86 (1): 19-27.
- Cate, P. C., 2007. Family Elateridae Leach, 1815 (Cebrioninae, Lissominae, Subprotelaterinae). Catalogue of Palaearctic Coleoptera, 4: 94-207.
- Cherry, R., 2007. Seasonal population dynamics of wireworms (Coleoptera: Elateridae) in Florida sugarcane fields. Florida Entomologist, 90 (3): 426-430.
- Etzler, F. E., K. W. Wanner, A. Morales-Rodriguez & M. A. Ivie, 2014. DNA barcoding to improve the species-level management of wireworms (Coleoptera: Elateridae). Journal of Economic Entomology, 107 (4): 1476-1485.
- Folmer, O., W. R. Hoeh, M. B. Black & R. C. Vrijenhoek, 1994. Conserved primers for PCR amplification of mitochondrial DNA from different invertebrate phyla. Molecular Marine Biology and Biotechnology, 3 (5): 294-299.
- Furlan, L. & M. Tóth, 2007. Occurrence of click beetle pest (Coleoptera, Elateridae) in Europe as detected by pheromone traps: Survey results of 1998-2006. IOBC/WPRS Bulletin, 30 (7): 1-19.
- Furlan, L., 1998. The biology of Agriotes ustulatus Schäller (Col., Elateridae). II. Larval development, pupation, whole cycle description and practical implications. Journal of Applied Entomology, 122 (1‐5): 71-78.
- Furlan, L., 2014. IPM thresholds for Agriotes wireworm species in maize in Southern Europe. Journal of Pest Science, 87 (4): 609-617.
- Furlan, L., I. Benvegnù, M. F. Bilò, J. Lehmhus & E. Ruzzier, 2021. Species identification of wireworms (Agriotes spp.; Coleoptera: Elateridae) of agricultural importance in Europe: A new "Horizontal Identification Table". Insects, 12 (6): 1-12.
- Gülperçin, N. & S. Tezcan, 2021. An Evaluation on Elateridae (Insecta: Coleoptera) Fauna of Eastern Anatolia Region. International Journal of Food Agriculture and Animal Sciences, 1 (1): 31-36.
- Kabalak, M. & O. Sert, 2011. Faunistic composition, ecological properties and zoogeographical composition of the family Elateridae (Coleoptera) of the Central Anatolian Region of Türkiye. Journal of Insect Science, 11 (1): 1-36.
- Kabalak, M. & O. Sert, 2021. Eleteridae (Coleoptera) of the Eastern Black Sea region of Türkiye: Fauna, some ecological evaluations, and zoogeographical composition. Transactions of the American Entomological Society, 147 (1): 157-191.
- Kabalak, M., 2018. Research on the family Elateridae (Coleoptera) of Türkiye: New distributional data, female descriptions and distributional evaluation. Transactions of the American Entomological Society, 144 (1): 143-166.
- Kabalak, M., O. Sert & D. Kondo, 2013. Faunistic composition, ecological properties, and zoogeographical composition of the Elateridae (Coleoptera) family in the Western Black Sea region of Türkiye. Journal of Insect Science, 13 (1): 1-21.
- Keiser, A., M. Häberli & P. Stamp, 2012. Quality deficiencies on potato (Solanum tuberosum L.) tubers caused by Rhizoctonia solani, wireworms (Agriotes ssp.) and slugs (Deroceras reticulatum, Arion hortensis) in different farming systems. Field Crops Research, 128: 147-155.
- Kroschel, J., N. Mujica, J. Okonya & A. Alyokhin, 2020. “Insect Pests Affecting Potatoes in Tropical, Subtropical, and Temperate Regions, 80-123”. In: The Potato Crop: Its Agricultural, Nutritional and Social Contribution to Humankind (Eds. H. Campos & O. Ortiz). Springer Nature, New York, 506 pp.
- Kuhar, T. P. & J. M. Alvarez, 2008. Timing of injury and efficacy of soil-applied insecticides against wireworms on potato in Virginia. Crop Protection, 27 (3-5): 792-798.
- Kumar, S., G. Stecher, M. Li, C. Knyaz & K. Tamura, 2018. MEGA X: molecular evolutionary genetics analysis across computing platforms. Molecular Biology and Evolution, 35 (6): 1547-1549.
- Lindroth, E. & T. L. Clark, 2009. Phylogenetic analysis of an economically important species complex of wireworms (Coleoptera: Elateridae) in the Midwest. Journal of Economic Entomology, 102 (2): 743-749.
- Lumeran, B. T., 2019. “Assemblage of Gastropods in the Rocky Intertidal Zone of Asry Beach, Kingdom of Bahrain, 125-147”. In: Invertebrates-Ecophysiology and Management (Eds. S. Ray, G. Diarte-Plata & R. Escamilla-Montes). Intech Open, London, 165 pp.
- Milosavljević, I., A. D. Esser & D. W. Crowder, 2017. Seasonal population dynamics of wireworms in wheat crops in the Pacific Northwestern United States. Journal of Pest Science, 90 (1): 77-86.
- Parker, W. E. & J. J. Howard, 2001. The biology and management of wireworms (Agriotes spp.) on potato with particular reference to the UK. Agricultural and Forest Entomology, 3 (2): 85-98.
- Poggi, S., R. Le Cointe, J. Lehmhus, M. Plantegenest & L. Furlan, 2021. Alternative strategies for controlling wireworms in field crops: A review. Agriculture, 11 (5): 1-30.
- Ritter, C. & E. Richter, 2013. Control methods and monitoring of Agriotes wireworms (Coleoptera: Elateridae). Journal of Plant Diseases and Protection, 120 (1): 4-15.
- Shannon, C. E., 1948. A mathematical theory of communication. The Bell System Technical Journal, 27 (3): 379-423.
- Staudacher, K., N. Schallhart, P. Pitterl, C. Wallinger, N. Brunner, M. Landl & M. Traugott, 2013. Occurrence of Agriotes wireworms in Austrian agricultural land. Journal of Pest Science, 86 (1): 33-39.
- Susurluk, I. A., 2008. Effects of various agricultural practices on persistence of the inundative applied entomopathogenic nematodes, Heterorhabditis bacteriophora and Steinernema feltiae in the field. Russian Journal of Nematology, 16 (1): 23-32.
- Tamura, K., M. Nei & S. Kumar, 2004. Prospects for inferring very large phylogenies by using the neighbor-joining method. Proceedings of the National Academy of Sciences, 101 (30): 11030-11035.
- Traugott, M., C. M. Benefer, R. P. Blackshaw, W. G. van Herk & R. S. Vernon, 2015. Biology, ecology, and control of elaterid beetles in agricultural land. Annual Review of Entomology, 60: 313-334.
- Zhang, S., Y. Liu, J. Shu, W. Zhang, Y. Zhang & H. Wang, 2019. DNA barcoding identification and genetic diversity of bamboo shoot wireworms (Coleoptera: Elateridae) in South China. Journal of Asia-Pacific Entomology, 22 (1): 140-150.
There are 31 citations in total.
Details
| Primary Language | English |
|---|---|
| Subjects | Entomology, Entomology in Agriculture, Plant Protection (Other) |
| Journal Section | Articles |
| Authors | |
| Project Number | Grant Project No: 115R025 |
| Early Pub Date | July 17, 2023 |
| Publication Date | July 20, 2023 |
| Submission Date | February 27, 2023 |
| Acceptance Date | July 7, 2023 |
| Published in Issue | Year 2023 Volume: 47 Issue: 2 |
