Research Article
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Year 2022, Volume: 56 Issue: 1, 49 - 54, 31.01.2022
https://doi.org/10.26650/eor.2022200002

Abstract

References

  • 1. Berkhoff JA, Chen PB, Teixeira FB, Diogenes A. Evaluation of triple antibiotic paste removal by different irrigation procedures. J Endod 2014;40(8):1172-7.
  • 2. Hargreaves KM, Diogenes A, Teixeira FB. Treatment options: biological basis of regenerative endodontic procedures. J Endod 2013;39(3 Suppl):S30-3.
  • 3. Ding RY, Cheung GS, Chen J, Yin XZ, Wang QQ, Zhang CF. Pulp revascularization of immature teeth with apical periodontitis: a clinical study. J Endod 2009;35(5):745-9.
  • 4. Jung IY, Lee SJ, Hargreaves KM. Biologically based treatment of immature permanent teeth with pulpal necrosis: a case series. Tex Dent J 2012;129(6):601-16.
  • 5. Scott MB, 2nd, Zilinski GS, Kirkpatrick TC, Himel VT, Sabey KA, Lallier TE. The effects of irrigants on the survival of human stem cells of the apical papilla, including endocyn. J Endod 2018;44(2):263-8.
  • 6. Althumairy RI, Teixeira FB, Diogenes A. Effect of dentin conditioning with intracanal medicaments on survival of stem cells of apical papilla. J Endod 2014;40(4):521-5.
  • 7. Ruparel NB, Teixeira FB, Ferraz CC, Diogenes A. Direct effect of intracanal medicaments on survival of stem cells of the apical papilla. J Endod 2012;38(10):1372-5.
  • 8. Sato I, Ando-Kurihara N, Kota K, Iwaku M, Hoshino E. Sterilization of infected root-canal dentine by topical application of a mixture of ciprofloxacin, metronidazole and minocycline in situ. Int Endod J 1996;29(2):118-24.
  • 9. Dabbagh B, Alvaro E, Vu DD, Rizkallah J, Schwartz S. Clinical complications in the revascularization of immature necrotic permanent teeth. Pediatr Dent 2012;34(5):414-7.
  • 10. Lenherr P, Allgayer N, Weiger R, Filippi A, Attin T, Krastl G. Tooth discoloration induced by endodontic materials: a laboratory study. Int Endod J 2012;45(10):942-9.
  • 11. Keskin C, Guler DH, Sariyilmaz E. Effect of intracanal time of triple antibiotic paste on its removal from simulated immature roots using passive ultrasonic irrigation and XP-endo Finisher. J Dent Res Dent Clin Dent Prospects 2018;12(4):288-93.
  • 12. Kirar DS, Jain P, Patni P. Comparison of different irrigation and agitation methods for the removal of two types of calcium hydroxide medicaments from the root canal wall: an in-vitro study. Clujul Med 2017;90(3):327-32.
  • 13. Devi AA, Abbott PV. Comparison of the flow characteristics of irrigants with standard and Max-i-Probe needles. Aust Endod J 2012;38(2):50-4.
  • 14. Silva PB, Krolow AM, Pilownic KJ, Casarin RP, Lima RK, Leonardo Rde T, et al. Apical extrusion of debris and irrigants using different irrigation needles. Braz Dent J 2016;27(2):192-5.
  • 15. Gokturk H, Ozkocak I, Buyukgebiz F, Demir O. An in vitro evaluation of various irrigation techniques for the removal of double antibiotic paste from root canal surfaces. J Appl Oral Sci 2016;24(6):568-4.
  • 16. Gokturk H, Ozkocak I, Buyukgebiz F, Demir O. Effectiveness of various irrigation protocols for the removal of calcium hydroxide from artificial standardized grooves. J Appl Oral Sci 2017;25(3):290-8.
  • 17. Yilmaz K, Tufenkci P, Adiguzel M. The effects of QMix and EndoActivator on postoperative pain in mandibular molars with nonvital pulps: a randomized clinical trial. Clin Oral Investig 2019;23(11):4173-80.
  • 18. Kucuk M, Kermeoglu F. Efficacy of different irrigation methods on dentinal tubule penetration of Chlorhexidine, QMix and Irritrol: A confocal laser scanning microscopy study. Aust Endod J 2019;45(2):202-8.
  • 19. Deniz Sungur D, Aksel H, Purali N. Effect of a low surface tension vehicle on the dentinal tubule penetration of calcium hydroxide and triple antibiotic paste. J Endod 2017;43(3):452-5.
  • 20. Oskoee SS, Bahari M, Daneshpooy M, Ajami AA, Rahbar M. Effect of Different Intraorifice Barriers and Bleaching Agents on the Fracture Resistance of Endodontically Treated Anterior Teeth. J Endod 2018;44(11):1731-5.
  • 21. Cochrane S, Burrow MF, Parashos P. Effect on the mechanical properties of human and bovine dentine of intracanal medicaments and irrigants. Aust Dent J 2019;64(1):35-42.
  • 22. Silva E, Carvalho NK, Prado MC, Senna PM, Souza EM, De-Deus G. Bovine teeth can reliably substitute human dentine in an intra-tooth push-out bond strength model? Int Endod J 2019;52(7):1063-9.
  • 23. Evans MD, Baumgartner JC, Khemaleelakul SU, Xia T. Efficacy of calcium hydroxide: chlorhexidine paste as an intracanal medication in bovine dentin. J Endod 2003;29(5):338-9.
  • 24. Sabrah AH, Yassen GH, Spolnik KJ, Hara AT, Platt JA, Gregory RL. Evaluation of Residual Antibacterial Effect of Human Radicular Dentin Treated with Triple and Double Antibiotic Pastes. J Endod 2015;41(7):1081-4.
  • 25. Bose R, Nummikoski P, Hargreaves K. A retrospective evaluation of radiographic outcomes in immature teeth with necrotic root canal systems treated with regenerative endodontic procedures. J Endod 2009;35(10):1343-9.
  • 26. Chuensombat S, Khemaleelakul S, Chattipakorn S, Srisuwan T. Cytotoxic effects and antibacterial efficacy of a 3-antibiotic combination: an in vitro study. J Endod 2013;39(6):813-9.
  • 27. Ustun Y, Duzgun S, Aslan T, Akti A. The efficiency of different irrigation solutions and techniques for the removal of triple antibiotic paste from simulated immature root canals. Niger J Clin Pract 2018;21(3):287-92.
  • 28. Galler KM, Widbiller M, Buchalla W, Eidt A, Hiller KA, Hoffer PC, et al. EDTA conditioning of dentine promotes adhesion, migration and differentiation of dental pulp stem cells. Int Endod J 2016;49(6):581-90.
  • 29. Galler KM, Krastl G, Simon S, Van Gorp G, Meschi N, Vahedi B, et al. European Society of Endodontology position statement: revitalization procedures. Int Endod J 2016;49(8):717-23.
  • 30. Boutsioukis C, Verhaagen B, Versluis M, Kastrinakis E, Wesselink PR, van der Sluis LW. Evaluation of irrigant flow in the root canal using different needle types by an unsteady computational fluid dynamics model. J Endod 2010;36(5):875-9.
  • 31. Akman M, Akbulut MB, Aydinbelge HA, Belli S. Comparison of different irrigation activation regimens and conventional irrigation techniques for the removal of modified triple antibiotic paste from root canals. J Endod 2015;41(5):720-4.
  • 32. Arslan H, Akcay M, Capar ID, Ertas H, Ok E, Uysal B. Efficacy of needle irrigation, EndoActivator, and photon-initiated photoacoustic streaming technique on removal of double and triple antibiotic pastes. J Endod 2014;40(9):1439-42.
  • 33. Ozyurek T, Demiryurek EO. Comparison of the effectiveness of different techniques for supportive removal of root canal filling material. Eur Endod J 2016;1(1):1-6.
  • 34. Boutsioukis C, Gogos C, Verhaagen B, Versluis M, Kastrinakis E, Van der Sluis LW. The effect of root canal taper on the irrigant flow: evaluation using an unsteady computational fluid dynamics model. Int Endod J 2010;43(10):909-16.
  • 35. Eymirli A, Nagas E, Uyanik MO, Cehreli ZC. Effect of laser-activated irrigation with ethylene diaminetetraacetic acid and phytic acid on the removal of calcium hydroxide and triple antibiotic paste from root dentin. Photomed Laser Surg 2017;35(1):43-8.
  • 36. Arslan H, Capar ID, Saygili G, Uysal B, Gok T, Ertas H, et al. Efficacy of various irrigation protocols on the removal of triple antibiotic paste. Int Endod J 2014;47(6):594-9.
  • 37. Ok E, Altunsoy M, Nur BG, Kalkan A. Effectiveness of different irrigation solutions on triple antibiotic paste removal from simulated immature root canal. Scanning 2015;37(6):409-13.
  • 38. Topcuoglu HS, Akti A, Topcuoglu G, Duzgun S, Ulusan O, Akpek F. Effectiveness of conventional syringe irrigation, vibringe, and passive ultrasonic irrigation performed with different irrigation regimes in removing triple antibiotic paste from simulated root canal irregularities. J Conserv Dent 2016;19(4):323-7.
  • 39. Turkaydin D, Demir E, Basturk FB, Sazak Ovecoglu H. Efficacy of XP-Endo Finisher in the removal of triple antibiotic paste from immature root canals. J Endod 2017;43(9):1528-31.
  • 40. Kaloustian MK, Nehme W, El Hachem C, Zogheib C, Ghosn N, Mallet JP, et al. Evaluation of two shaping systems and two sonic irrigation devices in removing root canal filling material from distal roots of mandibular molars assessed by micro CT. Int Endod J 2019;52(11):1635-44.
  • 41. Silva LJ, Pessoa OF, Teixeira MB, Gouveia CH, Braga RR. Micro-CT evaluation of calcium hydroxide removal through passive ultrasonic irrigation associated with or without an additional instrument. Int Endod J 2015;48(8):768-73.
  • 42. Zancan RF, Cavenago BC, Oda DF, Bramante CM, Andrade FB, Duarte MAH. Antimicrobial activity and physicochemical properties of antibiotic pastes used in regenerative endodontics. Braz Dent J 2019;30(6):536-41.
  • 43. Zancan RF, Vivan RR, Milanda Lopes MR, Weckwerth PH, de Andrade FB, Ponce JB, et al. Antimicrobial activity and physicochemical properties of calcium hydroxide pastes used as intracanal medication. J Endod 2016;42(12):1822-8.
  • 44. Genc Sen O, Kaya M. Comparative Safety of Needle, EndoActivator, and laser-activated irrigation in overinstrumented root canals. Photomed Laser Surg 2018;36(4):198-202.
  • 45. Yost RA, Bergeron BE, Kirkpatrick TC, Roberts MD, Roberts HW, Himel VT, et al. Evaluation of 4 different irrigating systems for apical extrusion of sodium hypochlorite. J Endod 2015;41(9):1530-4.
  • 46. Desai P, Himel V. Comparative safety of various intracanal irrigation systems. J Endod 2009;35(4):545-9.

Micro-computed tomography assessment of triple antibiotic paste removal using different irrigation methods

Year 2022, Volume: 56 Issue: 1, 49 - 54, 31.01.2022
https://doi.org/10.26650/eor.2022200002

Abstract

Purpose: The study aimed to compare four irrigation methods for triple antibiotic paste (TAP) removal using micro-computed tomography (micro-CT) analysis. Materials and Methods: Forty bovine central incisor teeth were selected, and the root canals were prepared up to #6 Peeso reamer drills. Equal portions of metronidazole, ciprofloxacin, and minocycline were used for the TAP preparation. The TAP was prepared by mixing the powder with distilled water (with a powder to liquid ratio of 1 mg/1 mL). The TAP was introduced to the canals with a lentulo spiral; then, the access cavities were temporarily sealed. After 21 days of storage, the teeth were randomly divided into four equal groups according to irrigation techniques: open-ended, side-vented, double side-vented needle irrigations and EndoActivator irrigation device. The TAP was removed using 17% EDTA (20 mL) and distilled water (5 mL) for all of the groups. The volume of the intracanal medicament before and after the irrigationprocedure was recorded by scanning the samples with micro-CT, and the TAP percentage was calculated. Results: The results showed that there was no statistically significant difference among the TAP percentage volumes removed by the different irrigation techniques. Conclusion: The irrigation techniques used in this study showed similar TAP removal efficiency, however, they could not completely remove the TAP from the root canal systems.

References

  • 1. Berkhoff JA, Chen PB, Teixeira FB, Diogenes A. Evaluation of triple antibiotic paste removal by different irrigation procedures. J Endod 2014;40(8):1172-7.
  • 2. Hargreaves KM, Diogenes A, Teixeira FB. Treatment options: biological basis of regenerative endodontic procedures. J Endod 2013;39(3 Suppl):S30-3.
  • 3. Ding RY, Cheung GS, Chen J, Yin XZ, Wang QQ, Zhang CF. Pulp revascularization of immature teeth with apical periodontitis: a clinical study. J Endod 2009;35(5):745-9.
  • 4. Jung IY, Lee SJ, Hargreaves KM. Biologically based treatment of immature permanent teeth with pulpal necrosis: a case series. Tex Dent J 2012;129(6):601-16.
  • 5. Scott MB, 2nd, Zilinski GS, Kirkpatrick TC, Himel VT, Sabey KA, Lallier TE. The effects of irrigants on the survival of human stem cells of the apical papilla, including endocyn. J Endod 2018;44(2):263-8.
  • 6. Althumairy RI, Teixeira FB, Diogenes A. Effect of dentin conditioning with intracanal medicaments on survival of stem cells of apical papilla. J Endod 2014;40(4):521-5.
  • 7. Ruparel NB, Teixeira FB, Ferraz CC, Diogenes A. Direct effect of intracanal medicaments on survival of stem cells of the apical papilla. J Endod 2012;38(10):1372-5.
  • 8. Sato I, Ando-Kurihara N, Kota K, Iwaku M, Hoshino E. Sterilization of infected root-canal dentine by topical application of a mixture of ciprofloxacin, metronidazole and minocycline in situ. Int Endod J 1996;29(2):118-24.
  • 9. Dabbagh B, Alvaro E, Vu DD, Rizkallah J, Schwartz S. Clinical complications in the revascularization of immature necrotic permanent teeth. Pediatr Dent 2012;34(5):414-7.
  • 10. Lenherr P, Allgayer N, Weiger R, Filippi A, Attin T, Krastl G. Tooth discoloration induced by endodontic materials: a laboratory study. Int Endod J 2012;45(10):942-9.
  • 11. Keskin C, Guler DH, Sariyilmaz E. Effect of intracanal time of triple antibiotic paste on its removal from simulated immature roots using passive ultrasonic irrigation and XP-endo Finisher. J Dent Res Dent Clin Dent Prospects 2018;12(4):288-93.
  • 12. Kirar DS, Jain P, Patni P. Comparison of different irrigation and agitation methods for the removal of two types of calcium hydroxide medicaments from the root canal wall: an in-vitro study. Clujul Med 2017;90(3):327-32.
  • 13. Devi AA, Abbott PV. Comparison of the flow characteristics of irrigants with standard and Max-i-Probe needles. Aust Endod J 2012;38(2):50-4.
  • 14. Silva PB, Krolow AM, Pilownic KJ, Casarin RP, Lima RK, Leonardo Rde T, et al. Apical extrusion of debris and irrigants using different irrigation needles. Braz Dent J 2016;27(2):192-5.
  • 15. Gokturk H, Ozkocak I, Buyukgebiz F, Demir O. An in vitro evaluation of various irrigation techniques for the removal of double antibiotic paste from root canal surfaces. J Appl Oral Sci 2016;24(6):568-4.
  • 16. Gokturk H, Ozkocak I, Buyukgebiz F, Demir O. Effectiveness of various irrigation protocols for the removal of calcium hydroxide from artificial standardized grooves. J Appl Oral Sci 2017;25(3):290-8.
  • 17. Yilmaz K, Tufenkci P, Adiguzel M. The effects of QMix and EndoActivator on postoperative pain in mandibular molars with nonvital pulps: a randomized clinical trial. Clin Oral Investig 2019;23(11):4173-80.
  • 18. Kucuk M, Kermeoglu F. Efficacy of different irrigation methods on dentinal tubule penetration of Chlorhexidine, QMix and Irritrol: A confocal laser scanning microscopy study. Aust Endod J 2019;45(2):202-8.
  • 19. Deniz Sungur D, Aksel H, Purali N. Effect of a low surface tension vehicle on the dentinal tubule penetration of calcium hydroxide and triple antibiotic paste. J Endod 2017;43(3):452-5.
  • 20. Oskoee SS, Bahari M, Daneshpooy M, Ajami AA, Rahbar M. Effect of Different Intraorifice Barriers and Bleaching Agents on the Fracture Resistance of Endodontically Treated Anterior Teeth. J Endod 2018;44(11):1731-5.
  • 21. Cochrane S, Burrow MF, Parashos P. Effect on the mechanical properties of human and bovine dentine of intracanal medicaments and irrigants. Aust Dent J 2019;64(1):35-42.
  • 22. Silva E, Carvalho NK, Prado MC, Senna PM, Souza EM, De-Deus G. Bovine teeth can reliably substitute human dentine in an intra-tooth push-out bond strength model? Int Endod J 2019;52(7):1063-9.
  • 23. Evans MD, Baumgartner JC, Khemaleelakul SU, Xia T. Efficacy of calcium hydroxide: chlorhexidine paste as an intracanal medication in bovine dentin. J Endod 2003;29(5):338-9.
  • 24. Sabrah AH, Yassen GH, Spolnik KJ, Hara AT, Platt JA, Gregory RL. Evaluation of Residual Antibacterial Effect of Human Radicular Dentin Treated with Triple and Double Antibiotic Pastes. J Endod 2015;41(7):1081-4.
  • 25. Bose R, Nummikoski P, Hargreaves K. A retrospective evaluation of radiographic outcomes in immature teeth with necrotic root canal systems treated with regenerative endodontic procedures. J Endod 2009;35(10):1343-9.
  • 26. Chuensombat S, Khemaleelakul S, Chattipakorn S, Srisuwan T. Cytotoxic effects and antibacterial efficacy of a 3-antibiotic combination: an in vitro study. J Endod 2013;39(6):813-9.
  • 27. Ustun Y, Duzgun S, Aslan T, Akti A. The efficiency of different irrigation solutions and techniques for the removal of triple antibiotic paste from simulated immature root canals. Niger J Clin Pract 2018;21(3):287-92.
  • 28. Galler KM, Widbiller M, Buchalla W, Eidt A, Hiller KA, Hoffer PC, et al. EDTA conditioning of dentine promotes adhesion, migration and differentiation of dental pulp stem cells. Int Endod J 2016;49(6):581-90.
  • 29. Galler KM, Krastl G, Simon S, Van Gorp G, Meschi N, Vahedi B, et al. European Society of Endodontology position statement: revitalization procedures. Int Endod J 2016;49(8):717-23.
  • 30. Boutsioukis C, Verhaagen B, Versluis M, Kastrinakis E, Wesselink PR, van der Sluis LW. Evaluation of irrigant flow in the root canal using different needle types by an unsteady computational fluid dynamics model. J Endod 2010;36(5):875-9.
  • 31. Akman M, Akbulut MB, Aydinbelge HA, Belli S. Comparison of different irrigation activation regimens and conventional irrigation techniques for the removal of modified triple antibiotic paste from root canals. J Endod 2015;41(5):720-4.
  • 32. Arslan H, Akcay M, Capar ID, Ertas H, Ok E, Uysal B. Efficacy of needle irrigation, EndoActivator, and photon-initiated photoacoustic streaming technique on removal of double and triple antibiotic pastes. J Endod 2014;40(9):1439-42.
  • 33. Ozyurek T, Demiryurek EO. Comparison of the effectiveness of different techniques for supportive removal of root canal filling material. Eur Endod J 2016;1(1):1-6.
  • 34. Boutsioukis C, Gogos C, Verhaagen B, Versluis M, Kastrinakis E, Van der Sluis LW. The effect of root canal taper on the irrigant flow: evaluation using an unsteady computational fluid dynamics model. Int Endod J 2010;43(10):909-16.
  • 35. Eymirli A, Nagas E, Uyanik MO, Cehreli ZC. Effect of laser-activated irrigation with ethylene diaminetetraacetic acid and phytic acid on the removal of calcium hydroxide and triple antibiotic paste from root dentin. Photomed Laser Surg 2017;35(1):43-8.
  • 36. Arslan H, Capar ID, Saygili G, Uysal B, Gok T, Ertas H, et al. Efficacy of various irrigation protocols on the removal of triple antibiotic paste. Int Endod J 2014;47(6):594-9.
  • 37. Ok E, Altunsoy M, Nur BG, Kalkan A. Effectiveness of different irrigation solutions on triple antibiotic paste removal from simulated immature root canal. Scanning 2015;37(6):409-13.
  • 38. Topcuoglu HS, Akti A, Topcuoglu G, Duzgun S, Ulusan O, Akpek F. Effectiveness of conventional syringe irrigation, vibringe, and passive ultrasonic irrigation performed with different irrigation regimes in removing triple antibiotic paste from simulated root canal irregularities. J Conserv Dent 2016;19(4):323-7.
  • 39. Turkaydin D, Demir E, Basturk FB, Sazak Ovecoglu H. Efficacy of XP-Endo Finisher in the removal of triple antibiotic paste from immature root canals. J Endod 2017;43(9):1528-31.
  • 40. Kaloustian MK, Nehme W, El Hachem C, Zogheib C, Ghosn N, Mallet JP, et al. Evaluation of two shaping systems and two sonic irrigation devices in removing root canal filling material from distal roots of mandibular molars assessed by micro CT. Int Endod J 2019;52(11):1635-44.
  • 41. Silva LJ, Pessoa OF, Teixeira MB, Gouveia CH, Braga RR. Micro-CT evaluation of calcium hydroxide removal through passive ultrasonic irrigation associated with or without an additional instrument. Int Endod J 2015;48(8):768-73.
  • 42. Zancan RF, Cavenago BC, Oda DF, Bramante CM, Andrade FB, Duarte MAH. Antimicrobial activity and physicochemical properties of antibiotic pastes used in regenerative endodontics. Braz Dent J 2019;30(6):536-41.
  • 43. Zancan RF, Vivan RR, Milanda Lopes MR, Weckwerth PH, de Andrade FB, Ponce JB, et al. Antimicrobial activity and physicochemical properties of calcium hydroxide pastes used as intracanal medication. J Endod 2016;42(12):1822-8.
  • 44. Genc Sen O, Kaya M. Comparative Safety of Needle, EndoActivator, and laser-activated irrigation in overinstrumented root canals. Photomed Laser Surg 2018;36(4):198-202.
  • 45. Yost RA, Bergeron BE, Kirkpatrick TC, Roberts MD, Roberts HW, Himel VT, et al. Evaluation of 4 different irrigating systems for apical extrusion of sodium hypochlorite. J Endod 2015;41(9):1530-4.
  • 46. Desai P, Himel V. Comparative safety of various intracanal irrigation systems. J Endod 2009;35(4):545-9.
There are 46 citations in total.

Details

Primary Language English
Subjects Dentistry
Journal Section Original Research Articles
Authors

Esma Sarıçam 0000-0001-7701-4214

Selen İnce Yusufoğlu 0000-0002-7826-6023

Meltem Kucuk 0000-0001-5073-4289

Ferhat Geneci 0000-0002-5039-4664

Mert Ocak 0000-0001-6832-6208

H. Hamdi Çelık 0000-0002-7909-7604

Publication Date January 31, 2022
Submission Date January 23, 2021
Published in Issue Year 2022 Volume: 56 Issue: 1

Cite

EndNote Sarıçam E, İnce Yusufoğlu S, Kucuk M, Geneci F, Ocak M, Çelık HH (January 1, 2022) Micro-computed tomography assessment of triple antibiotic paste removal using different irrigation methods. European Oral Research 56 1 49–54.