The
research include studying biochemical and genetic molecular study for
paraoxonase1 enzyme and identify some biochemical parameters for serum blood
samples 68 women with breast cancer and follow-up after take them three doses
of chemotherapy and comparative with 50 normal female as control group.The
result showed for women with breast cancer a significant increase in (TC,MDA
,Total protein, U,A , calcium and BMI) when compared with normal women, the
results also showed for women who have taken chemical doses and women with
breast cancer significant decreased in (PON1, GSH) compared with normal women,
the results showed for women who have taken
the first dose significant increase in (MDA,TP, Calcium, U.A , BMI),
while second dose and the third dose chemotherapy found significant increase in
(TP, U.A ,Calcium ) and significant decreased for (PON1). The results included
sequential analysis for PON1 enzyme ,it has shown results for genetic analyzed
selected exon (Exon4), where DNA extraction for normal women and breast cancer
women and taken their doses of chemotherapy and measure DNA concentration and
purity using Nano-Drop Spectrophotometric Analysis where the result show the
DNA concentration ranged between (5.2-68.4) ng/ml where concentration mean for
women with breast cancer (26.97) ng/ml and (17.49) ng/ml for normal women while
the first dose 24.74 ng/ml , (16.16-9.70)ng/ml for second and third dose
respectively . It has also been used PCR technique amplification and test products PCR analysis of sequential
technique by using gel electrophoresis where packet appeared clear without
deformation and tested PCR product sequencing for exon4 by capillary automated
sequencer and comparing the result with the normal sequence of the oxon.,
the results shows for women have taken first dose deletion mutation called
(Remove) frame shift mutation clear nitrogen base one lead to change all codons
after deletion site so that all amino acid change in poly protien chain which
led to the loss of amino acid (Tyr.) . and mutation type of silent mutation third mutation type of
substitution mutation (Replace amino acid (Thr.) by amino acid (Ser.).
| Primary Language | en |
|---|---|
| Journal Section | Articles |
| Authors | |
| Dates |
Publication Date : December 1, 2018 |
| Bibtex | @research article { epstem488727,
journal = {The Eurasia Proceedings of Science Technology Engineering and Mathematics},
issn = {},
eissn = {2602-3199},
address = {isresoffice@gmail.com},
publisher = {ISRES Publishing},
year = {2018},
volume = {},
pages = {56 - 71},
doi = {},
title = {Study of Paraoxonase1 Enzyme for Women with Breast Cancer (Biochemical and Molecular Genetics Study)},
key = {cite},
author = {Baker, Luma Abdalmunim and Hıdayet, Hazha Jamal and Al-chalabı, Nahida S.}
} |
| APA | Baker, L , Hıdayet, H , Al-chalabı, N . (2018). Study of Paraoxonase1 Enzyme for Women with Breast Cancer (Biochemical and Molecular Genetics Study) . The Eurasia Proceedings of Science Technology Engineering and Mathematics , (3) , 56-71 . Retrieved from https://dergipark.org.tr/en/pub/epstem/issue/40466/488727 |
| MLA | Baker, L , Hıdayet, H , Al-chalabı, N . "Study of Paraoxonase1 Enzyme for Women with Breast Cancer (Biochemical and Molecular Genetics Study)" . The Eurasia Proceedings of Science Technology Engineering and Mathematics (2018 ): 56-71 <https://dergipark.org.tr/en/pub/epstem/issue/40466/488727> |
| Chicago | Baker, L , Hıdayet, H , Al-chalabı, N . "Study of Paraoxonase1 Enzyme for Women with Breast Cancer (Biochemical and Molecular Genetics Study)". The Eurasia Proceedings of Science Technology Engineering and Mathematics (2018 ): 56-71 |
| RIS | TY - JOUR T1 - Study of Paraoxonase1 Enzyme for Women with Breast Cancer (Biochemical and Molecular Genetics Study) AU - Luma Abdalmunim Baker , Hazha Jamal Hıdayet , Nahida S. Al-chalabı Y1 - 2018 PY - 2018 N1 - DO - T2 - The Eurasia Proceedings of Science Technology Engineering and Mathematics JF - Journal JO - JOR SP - 56 EP - 71 VL - IS - 3 SN - -2602-3199 M3 - UR - Y2 - 2021 ER - |
| EndNote | %0 The Eurasia Proceedings of Science Technology Engineering and Mathematics Study of Paraoxonase1 Enzyme for Women with Breast Cancer (Biochemical and Molecular Genetics Study) %A Luma Abdalmunim Baker , Hazha Jamal Hıdayet , Nahida S. Al-chalabı %T Study of Paraoxonase1 Enzyme for Women with Breast Cancer (Biochemical and Molecular Genetics Study) %D 2018 %J The Eurasia Proceedings of Science Technology Engineering and Mathematics %P -2602-3199 %V %N 3 %R %U |
| ISNAD | Baker, Luma Abdalmunim , Hıdayet, Hazha Jamal , Al-chalabı, Nahida S. . "Study of Paraoxonase1 Enzyme for Women with Breast Cancer (Biochemical and Molecular Genetics Study)". The Eurasia Proceedings of Science Technology Engineering and Mathematics / 3 (December 2018): 56-71 . |
| AMA | Baker L , Hıdayet H , Al-chalabı N . Study of Paraoxonase1 Enzyme for Women with Breast Cancer (Biochemical and Molecular Genetics Study). EPSTEM. 2018; (3): 56-71. |
| Vancouver | Baker L , Hıdayet H , Al-chalabı N . Study of Paraoxonase1 Enzyme for Women with Breast Cancer (Biochemical and Molecular Genetics Study). The Eurasia Proceedings of Science Technology Engineering and Mathematics. 2018; (3): 56-71. |
| IEEE | L. Baker , H. Hıdayet and N. Al-chalabı , "Study of Paraoxonase1 Enzyme for Women with Breast Cancer (Biochemical and Molecular Genetics Study)", The Eurasia Proceedings of Science Technology Engineering and Mathematics, no. 3, pp. 56-71, Dec. 2018 |
