Erciyes Üniversitesi Veteriner Fakültesi Dergisi 1304-7280 2667-5498 Erciyes University 10.32707/ercivet.1728615 Veterinary Sciences (Other) Veteriner Bilimleri (Diğer) Evaluation of Serum Methylglyoxalate, D-lactate and L-lactate Levels in Calves with Acute Neonatal Diarrhea Akut Neonatal İshalli Buzağılarda Serum Metilglioksal, D-laktat ve L-laktat Düzeyinin Değerlendirilmesi https://orcid.org/0000-0001-9294-1825 Uztimür Murat BİNGÖL ÜNİVERSİTESİ https://orcid.org/0009-0009-2178-6157 Eskin Şevval BİNGÖL ÜNİVERSİTESİ https://orcid.org/0000-0001-8236-100X Keçeci Hakan BİNGÖL ÜNİVERSİTESİ https://orcid.org/0000-0002-8676-6490 Ünal Cennet Nur BİNGÖL ÜNİVERSİTESİ 04 29 2026 23 1 21 30 06 27 2025 03 26 2026 Copyright © 2004, Journal of The Faculty of Veterinary Medicine Erciyes University 2004 Journal of The Faculty of Veterinary Medicine Erciyes University

Lactate metabolism is critical for the healthy survival of animals. The imbalance of this metabolism leads to mortality. Therefore, the aim of this study was to evaluate the serum D-lactate, L-lactate, and serum methylglyoxalate concentrations in diarrheic calves infected with different infectious agents. Calves were grouped as rotavirus (n=15), coronavirus (n=15), and Cryptosporidium (n=15). The control group (n=15) calves were composed of calves determined to be healthy. While the serum methylglyoxalate and D-lactate levels of the control and diarrheic calves were determined by ELISA, the L-lactate level was determined by a blood gas analyzer. The D-lactate concentration of calves infected with rotavirus (P<0.002), coronavirus (P<0.01), and C. parvum (P<0.001) was significantly higher than that of the control group. The L-lactate concentration of the rotavirus (P<0.07) and coronavirus (P<0.006) infected calves was significantly higher than that of the control group. The White blood cell counts of the rotavirus (P<0.001), coronavirus (P<0.001), and C. parvum (P<0.001) calves were significantly higher than the control group. Unlike these, no statistically significant difference was found between the diarrhea calves in all groups and the control group in terms of serum methylglyoxalate concentration (P>0.499). In conclusion, in this study, it was found that D-lactate and L-lactate concentrations were significantly high in diarrhea caused by different infectious agents, and as a result, these metabolites should be taken into consideration and carefully considered in the evaluation of diarrhea in calves. Future studies are needed to evaluate the effects of D-lactate and L-lactate on mortality in calves with diarrhea of different severities.

Laktat metabolizması, hayvanların sağlıklı bir şekilde hayatta kalmaları için kritiktir. Bu metabolizmanın dengesizliği ölüme yol açar. Bu nedenle sunulan, bu çalışmanın amacı, farklı enfeksiyöz ajanlarla enfekte olmuş ishalli buzağılarda serum D-laktat, L-laktat ve serum metilgliozolat konsantrasyonlarını değerlendirmekti. Buzağılar rotavirüs (n=15), koronavirüs (n=15) ve Cryptosporidium (n=15) olarak gruplandırıldı. Kontrol grubu (n=15) sağlıklı olduğu belirlenen buzağılardan oluşuyordu. Kontrol ve ishalli buzağıların serum metilglioksalat ve D-laktat seviyeleri ELISA ile belirlenirken, L-laktat seviyesi kan gazı analizörü ile belirlenmiştir. Rotavirüs (P<0.002), koronavirüs (P<0.01) ve C. parvum (P<0.001) ile enfekte olmuş buzağıların D-laktat konsantrasyonu kontrol grubuna göre önemli ölçüde daha yüksekti. Rotavirüs (P<0.07) ve koronavirüs (P<0.006) ile enfekte olmuş buzağılardaki L-laktat konsantrasyonu kontrol grubuna göre önemli ölçüde yüksekti. Rotavirüs (P<0.001), koronavirüs (P<0.001) ve C. parvum (P<0.001) ile enfekte buzağıların beyaz kan hücresi sayıları kontrol grubuna göre anlamlı derecede yüksektir. Bunların aksine, tüm gruplardaki ishalli buzağılar ile kontrol grubu arasında serum metilglioxalat konsantrasyonu açısından istatistiksel olarak anlamlı bir fark bulunmamıştır (P>0.499). Sonuç olarak, bu çalışmada, farklı enfeksiyon etkenleri tarafından meydana gelen ishalde D-laktat ve L-laktat konsantrasyonlarının önemli ölçüde yüksek olduğu bulunmuş ve bu nedenle, bu metabolitlerin buzağılardaki ishalin değerlendirilmesinde dikkate alınması ve dikkatlice değerlendirilmesi gerektiği sonucuna varılmıştır. Gelecekteki çalışmalar, farklı şiddette ishal olan buzağılarda D-laktat ve L-laktatın mortalite üzerindeki etkilerini değerlendirmek için gereklidir.

 Buzağı ishal laktat metilglioksalat Calf diarrhea lactate methyglyoxalate This study has been financially supported by TÜBITAK-BİDEB-2209-a (Project Number: 1919B012326751). 1919B012326751 Adeva M, González-Lucán M, Seco M, Donapetry C. Enzymes involved in l-lactate metabolism in humans. Mitochondrion 2013; 13(6): 615-29. Akyüz E, Sezer M, Kuru M, Naseri A. Changes in hematology, some clinical biochemical parameters and mineral levels in neonatal calves with sepsis due to diarrhea. Van Vet J 2022; 33(1): 26-30. Allen SE, Holm JL. Lactate: physiology and clinical utility. J Vet Emerg Crit Care 2008; 18(2): 123-32. Berber E, Çanakoğlu N, Sözdutmaz İ, Simsek E, Sursal N, Ekinci G, Kökkaya S, Arıkan E, Ambarcıoğlu P, Göksu AG, Keleş İ. Seasonal and age-associated pathogen distribution in newborn calves with diarrhea admitted to ICU. Vet Sci 2021; 8(7): 128. Castagnetti C, Pirrone A, Mariella J, Mari G. Venous blood lactate evaluation in equine neonatal intensive care. Theriogenology 2010; 73(3): 343-57. Cho YI, Yoon KJ. An overview of calf diarrhea-infectious etiology, diagnosis, and intervention. J Vet Sci 2014; 15(1): 1. Chou CK, Lee YT, Chen SM, Hsieh CW, Huang TC, Li YC, Lee JA. Elevated urinary D-lactate levels in patients with diabetes and microalbuminuria. J Pharm Biomed Anal 2015; 116: 65-70. de Oliveira MR, de Souza IC, Brasil FB. Promotion of mitochondrial protection by emodin in methylglyoxal-treated human neuroblastoma SH-SY5Y cells: involvement of the AMPK/Nrf2/HO-1 axis. Neurotox 2021; 39(2): 292-304. Ekinci G, Tüfekçi E, Cissé Y, Bekdik İK, Onmaz AC, Aslan Ö, Güneş V, Çitil M, Keleş İ. Chloride and lactate as prognostic indicators of calf diarrhea from eighty-nine cases. J Vet Sci 2024; 25(3): e38. Figueiredo MD, Nydam DV, Perkins GA, Mitchell HM, Divers TJ. Prognostic value of plasma L‐lactate concentration measured cow‐side with a portable clinical analyzer in Holstein dairy cattle with abomasal disorders. J Vet Intern Med 2006; 20(6): 1463-70. Frandsen JR, Narayanasamy P. Neuroprotection through flavonoid: Enhancement of the glyoxalase pathway. Redox Biol 2018; 14: 465-73. Ider M, Maden M. Biomarkers of infectious pneumonia in naturally infected calves. Am J Vet Res 2022; 83(8): ajvr.21.10.0172. Keleş İ, Ekinci G, Tüfekçi E, Çitil M, Güneş V, Aslan Ö, Onmaz AC, Karaca Bekdik İ, Varol K, Deniz: Etiological and predisposing factors in calves with neonatal diarrhea: A clinical study in 270 case series. Kafkas Univ Vet Fak Derg 2022 28(3): 315-26. Kruse JA, Carlson RW. Lactate metabolism. Crit Care Clin 1987; 3(4): 725-46. Li C, Dai S, Lu J, Zhao B, Wang J, Li P, Wu Z, Mu Y, Feng C, Dong Q. Methylglyoxal: A newly detected and potentially harmful metabolite in the blood of ketotic dairy cows. J Dairy Sci 2018; 101(9): 8513- 23. Lorenz I. Influence of D‐lactate on metabolic acidosis and on prognosis in neonatal calves with diarrhoea. J Vet Med 2004; 51(9‐10): 425-8. Lorenz I, Trefz FM. Diarrhea in Calves. InProduction Diseases in Farm Animals: Pathophysiology, Prophylaxis and Health Management 2024; 26 (pp. 211-221). Cham: Springer International Publishing. Lu J, Randell E, Han Y, Adeli K, Krahn J, Meng QH. Increased plasma methylglyoxal level, inflammation, and vascular endothelial dysfunction in diabetic nephropathy. Clin Biochem 2011; 44(4): 307-11. Mariani CL, Nye CJ, Tokarz DA, Green L, Lau J, Zidan N, Early PJ, Guevar J, Muñana KR, Olby NJ, Miles S. Cerebrospinal fluid lactate in dogs with inflammatory central nervous system disorders. J Vet Intern Med 2019; 33(6): 2701-8. Matafome P, Sena C, Seiça R. Methylglyoxal, obesity, and diabetes. Endocrine 2013; 43: 472-84. Mizock BA, Falk JL. Lactic acidosis in critical illness. Crit Care Med 1992; 20(1): 80-93. Mizock BA. Lactate and point-of-care testing. Crit Care Med 1998; 26(9): 1474-6. Nemet I, Varga‐Defterdarović L, Turk Z. Methylglyoxal in food and living organisms. Mol Nutr Food Res 2006; 50(12): 1105-17. Nigro C, Leone A, Fiory F, Prevenzano I, Nicolò A, Mirra P, Beguinot F, Miele C. Dicarbonyl stress at the crossroads of healthy and unhealthy aging. Cells 2019; 8(7): 749. Ok M, Yildiz R, Hatipoglu F, Baspinar N, Ider M, Üney K, Ertürk A, Durgut MK, Terzi F. Use of intestine-related biomarkers for detecting intestinal epithelial damage in neonatal calves with diarrhea. Am J Vet Res 2020; 81(2): 139-46. Omole OO, Nappert G, Naylor JM, Zello GA. Both L-and D-lactate contribute to metabolic acidosis in diarrheic calves. J Nutr 2001; 131(8): 2128-31. Osorio JS. Gut health, stress, and immunity in neonatal dairy calves: the host side of host-pathogen interactions. J Anim Sci Biotechnol 2020; 11(1): 105. Pang DS, Boysen S. Lactate in veterinary critical care: pathophysiology and management. J Am Anim Hosp Assoc 2007; 43(5): 270-9. Prestes AD, Dos Santos MM, Ecker A, Zanini D, Schetinger MR, Rosemberg DB, da Rocha JB, Barbosa NV. Evaluation of methylglyoxal toxicity in human erythrocytes, leukocytes and platelets. Toxicol Mech Methods 2017; 27(4): 307-17. Radcliffe RM, Divers TJ, Fletcher DJ, Mohammed H, Kraus MS. Evaluation of L‐lactate and cardiac troponin I in horses undergoing emergency abdominal surgery. J Vet Emerg Crit Care 2012; 22(3): 313-9. Schumacher, D., Morgenstern, J., Oguchi, Y., Volk, N., Kopf, S., Groener, J. B., Freichel, M. Compensatory mechanisms for methylglyoxal detoxification in experimental & clinical diabetes. Mol Metab 2018; 143-52. Uztimür M. Diagnostic significance of mean platelet volume and erythrocyte distribution width in calves with sepsis. Erciyes Üniv Vet Fak Derg 2024; 21(3): 194-201. Uztimür M, Kizil Ö, Akbulut HH. Immunophenotyping of peripheral circulating lymphocytes and serum selenium levels in calves with neonatal diarrhea. Vet Immunol Immunopathol 2024; 269: 110728. Yin, P., Lehmann, R., Xu, G. Effects of pre-analytical processes on blood samples used in metabolomics studies. Anal Bioanal Chem 2015; 407(17): 4879-92. Zhang S, Jiao T, Chen Y, Gao N, Zhang L, Jiang M. Methylglyoxal induces systemic symptoms of irritable bowel syndrome. PloS one 2014; 9(8): e105307.