The Inhibition Effects of Eugenol and Pulegone on Stenotrophomonas maltophilia: an Opportunistic Pathogen

Year 2019, Volume: 16 Issue: 1, 23 - 29, 25.03.2019

Tuba Baygar

https://doi.org/10.32707/ercivet.538021

Cited By: 4

Abstract

Aerobic,
non-fermentative and Gram-negative Stenotrophomonas
maltophilia is a multidrug-resistant bacilli that is known to be
pathogen for human and animals. S.
maltophilia has been isolated from different animal species and also found
in a variety of environments including soil, water, and plants. S. maltophilia, which has the ability to form biofilms
on surfaces that cause environmental problems, is
resistant to many antibiotic classes such as cephalosporins, carbapenems, and
aminoglycosides. Here in this study, its
aimed to determine the inhibition activities of natural phenolic compounds eugenol and
pulegone against S. maltophilia MU69.
Antibacterial activities of eugenol and pulegone were initially determined by
disc diffusion method. Minimum Inhibitory Concentration (MIC) and Minimum
Bactericidal Concentration (MBC) were also determined by tube dilution assay.
Antibiofilm activities of the compounds were investigated by crystal violet
staining and also monitorized using Scanning Electron Microscopy (SEM). Zone of
inhibition measurements were found to be 39 mm and 28 mm for eugenol and
pulegone, respectively. MIC and MBC values were found to be 3.12 mg/mL for
eugenol while these were 2.5 mg/mL and 5 mg/mL for pulegone, respectively. The
highest antibiofilm activity was found to be 56.30±0.45% for MIC of eugenol. To
our knowledge, this is the first report of the antibacterial and antibiofilm
activity of eugenol and pulegone against S.
maltophilia. According to the biological activity results, it can be
concluded that these natural agents may be potentially used for veterinary
sciences, food industry or pharmaceutical applications that aim to manage S. maltophilia biofilm.

Keywords

Antibacterial, antibiofilm, eugenol, pulegone, S. maltophilia

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