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Mısır Silajına Ticari (Bonsilage-Mais) ve Doğal Laktik Asit Bakteri İnokulantı Katkısının Silaj Kalitesi Üzerine Etkisi

Year 2022, Volume: 19 Issue: 1, 24 - 29, 01.04.2022
https://doi.org/10.32707/ercivet.1085234

Abstract

Bu çalışma mısır silajına ticari laktik asit bakteri (LAB) inokulantı ve doğal fermente edilmiş laktik asit bakteri sıvısı (Pre-FermentedJuice-PFJ) katkılarının silaj kalitesi üzerine etkilerini belirlemek amacıyla yapılmıştır. Silaj grupları, katkısız grubun yanı sıra ticari LAB inokulantı (Bonsilage-Mais, 2.5x105cfu/g taze materyal), %0.1 (3x105cfu/g taze materyal), %0.3 (9x105 cfu/g taze materyal) ve %0.5 (1.5x106cfu/g taze materyal) PFJ ilave edilerek oluşturulmuştur. Her bir silaj muamele grubu dörder tekerrür olacak şekilde hazırlanmıştır. Kontrol grubu ile kıyaslandığında; ticari LAB inokulantı ile %0.3 ve %0.5 PFJ katkılarının silajların kuru madde içeriklerini azalttıkları, ticari LAB inokulantı katkısının ham protein içeriğini arttırdığı (P<0.05) tespit edilmiştir. Kontrol silajı ile kıyaslandığında; ticari LAB inokulantı ile PFJ katkılarının (%0.1, %0.3 ve %0.5) silaj amonyak azotu (NH3-N/TN), laktik asit (LA) ve asetik asit (AA) değerlerini arttırdıkları görülmüştür (P<0.05). Bu çalışma kapsamında hazırlanan silajların hiçbirinde bütirik asit (BA) tespit edilmemiştir. Sonuç olarak, silaj fermantasyonunu güçlendirmesi adına mısır bitkisinden hazırlanacak silajlara laktik asit ve asetik asit değerlerini arttırmalarından dolayı ticari LAB inokulantı yerine %0.3 veya %0.5 PFJ katkılarının kullanılabileceği kanaatine varılmıştır.

References

  • Aksu T, Baytok E, Bolat D. Effects of a bacterial silage inoculant on corn silage fermentation and nutrient digestibility. Small Rumin Res 2004;55(1-3): 249-52.
  • Arslan C, Tufan T, Avci M, Kaplan O, Uyarlar C. Effects of molasses, barley, oak tannıns extracts and prevıously fermented juıce addıtıon on silage characteristics, in vitro organic matter digestibility and metabolisable energy content of grass silage. Fresenius Environ Bull 2020; 29(8): 6533-42.
  • AOAC. Association of Official Analytical Chemistry. Official Methods of Analysis of AOAC International, Eighteenth Edition. Association of Official Analytical Chemists, Washington DC, 2005, USA.
  • Broderick GA, Kang JH. Automated simultaneous determination of ammonia and total amino acids in ruminal fluid andin vitro media. J Dairy Sci 1980; 63: 64-75.
  • Carpintero CM, Henderson AR, McDonald P. The effect of some pre-treatments on proteolysis during the ensiling of herbage. Grass Forage Sci 1979; 34: 311-5.
  • Denek N, Can A, Avci M, Aksu T. The effect of fresh and frozen pre-fermented juice on the fermentation quality of alfalfa silage. Kafkas Univ Vet Fak Derg 2012; 18: 785-90.
  • Denek N, Can A, Avci M, Aksu T, Durmaz H. The effect of molassesbased pre‐fermented juice on the fermentation quality of first‐cut lucerne silage. Grass Forage Sci 2011; 66(2): 243-50.
  • Driehuis F, Elferink SO, Spoelstra SF. Anaerobic lactic acid degradation during ensilage of whole crop maize inoculated with Lactobacillus buchneri inhibits yeast growth and improves aerobic stability. J Appl Microbiol 1999; 87(4): 583-94.
  • Duni`ere L, Sindou J, Chaucheyras-Durve F, Chevallier I, Th´evenot-Sergentet D. Silage processing and strategies to prevent persistence of undesirable microorganisms. Anim Feed Sci Tech 2013; 182: 1-15.
  • Filya İ. Effects of some silage additives on ruminant performance. J Anim Prod 2000; 41: 76-83.
  • Filya İ, Sucu E, Karabulut A. The Effect of Lactobacillus buchneri on the fermentation, aerobic stability and ruminal degradability of maize silage. J Appl Microbiol 2006; 101: 1216-23.
  • Güney M, Kale C, Temur C, Bingol NT, Aksu T. The effect of molasses and pre-fermented lactic acid juice on silage quality and in vitro digestibility of sugar beet pulp + wheat straw silage. Fresenius Environ Bull2018; 27(11): 7454-9.
  • Kayembe K, Basosila L, Mpiana PT, Sikulisimwa PC,Mbuyu K. Inhibitory effects of phenolic monomers on methanogenesis in anaerobic digestion. Int J Res Microbiol 2013; 3 (1): 32-41.
  • Kilic A. Silage Feed. Bilgehan Press, Izmir, Turkey, 1984; p. 350.
  • Kleinschmit DH, Schmidt RJ, Kung Jr L. The effects of various antifungal additives on the fermentation and aerobic stability of corn silage. J Dairy Sci 2005; 88(6): 2130-9.
  • Kung L, Shaver R. Interpretation and use of silage fermentation analysis reports. Focus on Forage. 2001; 3(13): 1-5.
  • Masuko T, Hariyama Y, Takahashi Y, Cao LM, Goto M, Ohshıma M. Effect of addition of fermented juice of epiphytic lactic acid bacteria prepared from timothy and orchard grass on fermentation quality of silages. J Grassland Sci (Japan) 2002; 48(2): 120-5.
  • Masuko T, Okada S, Uchimura T, Awaya K. Effects of inoculation with lactic acid bacteria culture at ensiling on the fermentative quality and flora of lactic acid bacteria of grass silage. Anim Feed Sci Technol 1992; 63: 1182-7.
  • Meeske R, Basson HM. The Effects of a lactic acid bacteria ınoculant on maize silage. Anim Feed Sci Technol 1998; 70: 239-47.
  • Nishino N, Uchida S. Laboratory evaluation of previously fermented juice as a fermentation stimulant for lucerne silage. J Sci Food Agric 1999; 79: 1285-8.
  • Nishino N, Yoshida M, Shiota H, Sakaguchi E. Accumulation of 1,2-propanediol and enhancement of aerobic stability in whole crop maize silage inoculated with Lactobacillus buchneri. J Appl Microbiol 2003; 94: 800-7.
  • Nishino N, Wada H, Yoshida M, Shiota H. Microbial counts, fermentation products, and aerobic stability of whole crop corn and a total mixed ration ensiled with and without inoculation of Lactobacillus casei or Lactobacillus buchneri. J Dairy Sci 2004; 87(8): 2563-70.
  • Nkosi BD, Meeske R, Palic D, Langa T. Laboratory evaluation of an inoculant for ensiling whole crop maize in South Africa. Anim Feed Sci Technol 2009; 150(1-2): 144-50.
  • Ohshima M, Cao LM, Kimura E, Ohshima Y, Yokoto HO. Influence of addition of previously fermented juice to alfalfa ensiled at different moisture contents. J Grassland Sci (Japan) 1997a; 43(1): 56-8.
  • Ohshima M, Kimura E, Yokota H. A method of making good quality silage from direct cut alfalfa by spraying previously fermented juice. Anim Feed Sci Technol 1997b; 66: 129-37.
  • Palic D, Vukmirovic DJ, Colovic R, Kokic B, Cabarkapa I, Ivanov D, Okanovic DJ. The effect of a bacterial inoculant on fermentation, microbial status and aerobic stability of whole crop maize silage. Rom Biotechnol Lett 2011; 16(3): 6256-62.
  • Polan CE, Stieve DE, Garrett JL. Protein preservation and ruminal degradation of ensiled forage treated with heat, formic acid, ammonia, or microbial inoculant. J Dairy Sci 1998; 81: 765-76.
  • Rabaioli Rama G, Kuhn D, Beux S, Jachetti M, Maciel MJ, Volken de Souza CF. Cheese whey and ricotta whey for the growth and encapsulation of endogenous lactic acid bacteria. Food Bioproc Tech 2020; 13: 308-22.
  • Ranjit NK, Kung Jr L. The effect of Lactobacillus buchneri, Lactobacillus plantarum, or a chemical preservative on the fermentation and aerobic stability of corn silage. J Dairy Sci 2000; 83(3): 526-35.
  • Shao T, Zhang L, Shimojo M, Masuda Y. Fermentation quality of Italian ryegrass (Loliummultiflorum Lam.) silages treated with encapsulated-glucose, glucose, sorbic acid and pre-fermented juices. Asian-Australas J AnimSci 2007; 20(11): 1699-704.
  • Slottner D, Bertilsson J. Effect of ensiling technology on protein degradation during ensilage. Anim Feed SciTechnol 2006; 127(1–2): 101-11.
  • SPSS, 1991: Inc. Statistical package for the social sciences (SPSS/PC+). Chicago, IL.
  • Suzuki M, Lund CW. Improved gas liquid chromatography for simultaneous determination of volatile fatty acids and lactic acid in silage. J Agric Food Chem 1980; 28: 1040-1.
  • Tao X, Chen S, Zhao J, Wang S, Dong Z, Li J, Shao T. Effects of citric acid residue and lactic acid bacteria on fermentation quality and aerobic stability of alfalfa silage. Ital J Anim Sci 2020; 19(1): 744-52.
  • Van Soest PJ, Robertson JB, Lewis BA. Methods of dietary fiber, neutral detergent fiber and non starch polysaccharides in relation to animal nutrition. J Dairy Sci 1991; 74: 3583-97.
  • Wang J, Wang JQ, Zhou H, Feng T. Effects of addition of previously fermented juice prepared from alfalfa on fermentation quality and protein degradation of alfalfa silage. Anim Feed Sci Technol 2009; 151: 280-90.
  • Weinberg ZG, Ashbell G, Azrieli A. The effect of applying lactic bacteria in ensiling on the chemical and microbiological composition of vetch, wheat and alfalfa silages. J Appl Bacteriol1988; 64:1-8.

Effect of Supplementing Corn Silage with Commercial (Bonsilage-Mais) and Pre-Fermented Lactic Acid Bacteria Inoculant on Silage Quality

Year 2022, Volume: 19 Issue: 1, 24 - 29, 01.04.2022
https://doi.org/10.32707/ercivet.1085234

Abstract

This study was aimed to determine the effects of supplementing corn silage with commercial lactic acid bacteria (LAB) inoculant and pre-fermented LAB juice (PFJ) on silage quality. Silage groups other than the control group were designed by adding commercial LAB inoculant (Bonsilage-Mais, 2.5 × 105cfu/g of fresh material), and 0.1% (3× 05cfu/g of fresh material), 0.3% (9×105cfu/g of fresh material), and 0.5% (1.5×106cfu/g of fresh material) PFJ. Each silage treatment group was prepared in four replicates. When compared to the control group, the addition of 0.3%, 0.5% PFJ and commercial LAB inoculant decreased the dry matter content of the silages, while the commercial LAB inoculant increased the crude protein content (P<0.05). When compared to the control silage, commercial LAB inoculant and PFJ additives (0.1%, 0.3%, and 0.5%) increased the ammonia nitrogen, lactic acid (LA), and acetic acid (AA) values of the silages (P<0.05). Butyric acid (BA) was not detected in the silages. As a result, it was concluded that 0.3% or 0.5% PFJ additives can be used as silage additives instead of commercial LAB inoculant.

References

  • Aksu T, Baytok E, Bolat D. Effects of a bacterial silage inoculant on corn silage fermentation and nutrient digestibility. Small Rumin Res 2004;55(1-3): 249-52.
  • Arslan C, Tufan T, Avci M, Kaplan O, Uyarlar C. Effects of molasses, barley, oak tannıns extracts and prevıously fermented juıce addıtıon on silage characteristics, in vitro organic matter digestibility and metabolisable energy content of grass silage. Fresenius Environ Bull 2020; 29(8): 6533-42.
  • AOAC. Association of Official Analytical Chemistry. Official Methods of Analysis of AOAC International, Eighteenth Edition. Association of Official Analytical Chemists, Washington DC, 2005, USA.
  • Broderick GA, Kang JH. Automated simultaneous determination of ammonia and total amino acids in ruminal fluid andin vitro media. J Dairy Sci 1980; 63: 64-75.
  • Carpintero CM, Henderson AR, McDonald P. The effect of some pre-treatments on proteolysis during the ensiling of herbage. Grass Forage Sci 1979; 34: 311-5.
  • Denek N, Can A, Avci M, Aksu T. The effect of fresh and frozen pre-fermented juice on the fermentation quality of alfalfa silage. Kafkas Univ Vet Fak Derg 2012; 18: 785-90.
  • Denek N, Can A, Avci M, Aksu T, Durmaz H. The effect of molassesbased pre‐fermented juice on the fermentation quality of first‐cut lucerne silage. Grass Forage Sci 2011; 66(2): 243-50.
  • Driehuis F, Elferink SO, Spoelstra SF. Anaerobic lactic acid degradation during ensilage of whole crop maize inoculated with Lactobacillus buchneri inhibits yeast growth and improves aerobic stability. J Appl Microbiol 1999; 87(4): 583-94.
  • Duni`ere L, Sindou J, Chaucheyras-Durve F, Chevallier I, Th´evenot-Sergentet D. Silage processing and strategies to prevent persistence of undesirable microorganisms. Anim Feed Sci Tech 2013; 182: 1-15.
  • Filya İ. Effects of some silage additives on ruminant performance. J Anim Prod 2000; 41: 76-83.
  • Filya İ, Sucu E, Karabulut A. The Effect of Lactobacillus buchneri on the fermentation, aerobic stability and ruminal degradability of maize silage. J Appl Microbiol 2006; 101: 1216-23.
  • Güney M, Kale C, Temur C, Bingol NT, Aksu T. The effect of molasses and pre-fermented lactic acid juice on silage quality and in vitro digestibility of sugar beet pulp + wheat straw silage. Fresenius Environ Bull2018; 27(11): 7454-9.
  • Kayembe K, Basosila L, Mpiana PT, Sikulisimwa PC,Mbuyu K. Inhibitory effects of phenolic monomers on methanogenesis in anaerobic digestion. Int J Res Microbiol 2013; 3 (1): 32-41.
  • Kilic A. Silage Feed. Bilgehan Press, Izmir, Turkey, 1984; p. 350.
  • Kleinschmit DH, Schmidt RJ, Kung Jr L. The effects of various antifungal additives on the fermentation and aerobic stability of corn silage. J Dairy Sci 2005; 88(6): 2130-9.
  • Kung L, Shaver R. Interpretation and use of silage fermentation analysis reports. Focus on Forage. 2001; 3(13): 1-5.
  • Masuko T, Hariyama Y, Takahashi Y, Cao LM, Goto M, Ohshıma M. Effect of addition of fermented juice of epiphytic lactic acid bacteria prepared from timothy and orchard grass on fermentation quality of silages. J Grassland Sci (Japan) 2002; 48(2): 120-5.
  • Masuko T, Okada S, Uchimura T, Awaya K. Effects of inoculation with lactic acid bacteria culture at ensiling on the fermentative quality and flora of lactic acid bacteria of grass silage. Anim Feed Sci Technol 1992; 63: 1182-7.
  • Meeske R, Basson HM. The Effects of a lactic acid bacteria ınoculant on maize silage. Anim Feed Sci Technol 1998; 70: 239-47.
  • Nishino N, Uchida S. Laboratory evaluation of previously fermented juice as a fermentation stimulant for lucerne silage. J Sci Food Agric 1999; 79: 1285-8.
  • Nishino N, Yoshida M, Shiota H, Sakaguchi E. Accumulation of 1,2-propanediol and enhancement of aerobic stability in whole crop maize silage inoculated with Lactobacillus buchneri. J Appl Microbiol 2003; 94: 800-7.
  • Nishino N, Wada H, Yoshida M, Shiota H. Microbial counts, fermentation products, and aerobic stability of whole crop corn and a total mixed ration ensiled with and without inoculation of Lactobacillus casei or Lactobacillus buchneri. J Dairy Sci 2004; 87(8): 2563-70.
  • Nkosi BD, Meeske R, Palic D, Langa T. Laboratory evaluation of an inoculant for ensiling whole crop maize in South Africa. Anim Feed Sci Technol 2009; 150(1-2): 144-50.
  • Ohshima M, Cao LM, Kimura E, Ohshima Y, Yokoto HO. Influence of addition of previously fermented juice to alfalfa ensiled at different moisture contents. J Grassland Sci (Japan) 1997a; 43(1): 56-8.
  • Ohshima M, Kimura E, Yokota H. A method of making good quality silage from direct cut alfalfa by spraying previously fermented juice. Anim Feed Sci Technol 1997b; 66: 129-37.
  • Palic D, Vukmirovic DJ, Colovic R, Kokic B, Cabarkapa I, Ivanov D, Okanovic DJ. The effect of a bacterial inoculant on fermentation, microbial status and aerobic stability of whole crop maize silage. Rom Biotechnol Lett 2011; 16(3): 6256-62.
  • Polan CE, Stieve DE, Garrett JL. Protein preservation and ruminal degradation of ensiled forage treated with heat, formic acid, ammonia, or microbial inoculant. J Dairy Sci 1998; 81: 765-76.
  • Rabaioli Rama G, Kuhn D, Beux S, Jachetti M, Maciel MJ, Volken de Souza CF. Cheese whey and ricotta whey for the growth and encapsulation of endogenous lactic acid bacteria. Food Bioproc Tech 2020; 13: 308-22.
  • Ranjit NK, Kung Jr L. The effect of Lactobacillus buchneri, Lactobacillus plantarum, or a chemical preservative on the fermentation and aerobic stability of corn silage. J Dairy Sci 2000; 83(3): 526-35.
  • Shao T, Zhang L, Shimojo M, Masuda Y. Fermentation quality of Italian ryegrass (Loliummultiflorum Lam.) silages treated with encapsulated-glucose, glucose, sorbic acid and pre-fermented juices. Asian-Australas J AnimSci 2007; 20(11): 1699-704.
  • Slottner D, Bertilsson J. Effect of ensiling technology on protein degradation during ensilage. Anim Feed SciTechnol 2006; 127(1–2): 101-11.
  • SPSS, 1991: Inc. Statistical package for the social sciences (SPSS/PC+). Chicago, IL.
  • Suzuki M, Lund CW. Improved gas liquid chromatography for simultaneous determination of volatile fatty acids and lactic acid in silage. J Agric Food Chem 1980; 28: 1040-1.
  • Tao X, Chen S, Zhao J, Wang S, Dong Z, Li J, Shao T. Effects of citric acid residue and lactic acid bacteria on fermentation quality and aerobic stability of alfalfa silage. Ital J Anim Sci 2020; 19(1): 744-52.
  • Van Soest PJ, Robertson JB, Lewis BA. Methods of dietary fiber, neutral detergent fiber and non starch polysaccharides in relation to animal nutrition. J Dairy Sci 1991; 74: 3583-97.
  • Wang J, Wang JQ, Zhou H, Feng T. Effects of addition of previously fermented juice prepared from alfalfa on fermentation quality and protein degradation of alfalfa silage. Anim Feed Sci Technol 2009; 151: 280-90.
  • Weinberg ZG, Ashbell G, Azrieli A. The effect of applying lactic bacteria in ensiling on the chemical and microbiological composition of vetch, wheat and alfalfa silages. J Appl Bacteriol1988; 64:1-8.
There are 37 citations in total.

Details

Primary Language English
Journal Section Articles
Authors

Besime Doğan Daş This is me 0000-0003-2163-2632

Nihat Denek This is me 0000-0003-0904-8943

Gülçin Baytur 0000-0002-1878-3862

Publication Date April 1, 2022
Submission Date June 16, 2021
Acceptance Date November 29, 2021
Published in Issue Year 2022 Volume: 19 Issue: 1

Cite

APA Doğan Daş, B., Denek, N., & Baytur, G. (2022). Effect of Supplementing Corn Silage with Commercial (Bonsilage-Mais) and Pre-Fermented Lactic Acid Bacteria Inoculant on Silage Quality. Erciyes Üniversitesi Veteriner Fakültesi Dergisi, 19(1), 24-29. https://doi.org/10.32707/ercivet.1085234
AMA Doğan Daş B, Denek N, Baytur G. Effect of Supplementing Corn Silage with Commercial (Bonsilage-Mais) and Pre-Fermented Lactic Acid Bacteria Inoculant on Silage Quality. Erciyes Üniv Vet Fak Derg. April 2022;19(1):24-29. doi:10.32707/ercivet.1085234
Chicago Doğan Daş, Besime, Nihat Denek, and Gülçin Baytur. “Effect of Supplementing Corn Silage With Commercial (Bonsilage-Mais) and Pre-Fermented Lactic Acid Bacteria Inoculant on Silage Quality”. Erciyes Üniversitesi Veteriner Fakültesi Dergisi 19, no. 1 (April 2022): 24-29. https://doi.org/10.32707/ercivet.1085234.
EndNote Doğan Daş B, Denek N, Baytur G (April 1, 2022) Effect of Supplementing Corn Silage with Commercial (Bonsilage-Mais) and Pre-Fermented Lactic Acid Bacteria Inoculant on Silage Quality. Erciyes Üniversitesi Veteriner Fakültesi Dergisi 19 1 24–29.
IEEE B. Doğan Daş, N. Denek, and G. Baytur, “Effect of Supplementing Corn Silage with Commercial (Bonsilage-Mais) and Pre-Fermented Lactic Acid Bacteria Inoculant on Silage Quality”, Erciyes Üniv Vet Fak Derg, vol. 19, no. 1, pp. 24–29, 2022, doi: 10.32707/ercivet.1085234.
ISNAD Doğan Daş, Besime et al. “Effect of Supplementing Corn Silage With Commercial (Bonsilage-Mais) and Pre-Fermented Lactic Acid Bacteria Inoculant on Silage Quality”. Erciyes Üniversitesi Veteriner Fakültesi Dergisi 19/1 (April 2022), 24-29. https://doi.org/10.32707/ercivet.1085234.
JAMA Doğan Daş B, Denek N, Baytur G. Effect of Supplementing Corn Silage with Commercial (Bonsilage-Mais) and Pre-Fermented Lactic Acid Bacteria Inoculant on Silage Quality. Erciyes Üniv Vet Fak Derg. 2022;19:24–29.
MLA Doğan Daş, Besime et al. “Effect of Supplementing Corn Silage With Commercial (Bonsilage-Mais) and Pre-Fermented Lactic Acid Bacteria Inoculant on Silage Quality”. Erciyes Üniversitesi Veteriner Fakültesi Dergisi, vol. 19, no. 1, 2022, pp. 24-29, doi:10.32707/ercivet.1085234.
Vancouver Doğan Daş B, Denek N, Baytur G. Effect of Supplementing Corn Silage with Commercial (Bonsilage-Mais) and Pre-Fermented Lactic Acid Bacteria Inoculant on Silage Quality. Erciyes Üniv Vet Fak Derg. 2022;19(1):24-9.