Kültüre Edilen Sivriburun Karagöz Balıklarında (Diplodus puntazzo Cetti, 1777) Micrococcus luteus Enfeksiyonunun Teşhisi

Abstract

Bu çalışmada Ege Denizi'nde kültüre edilen sivriburun karagöz balıklarında (Diplodus puntazzo Cetti, 1777) meydana gelen ölümlerin nedeninin belirlenmesi amaçlandı. Mayıs 2009 ve Mayıs 2010 tarihleri ​​arasında bakteriyolojik yöntemler kullanılarak toplam 10 hasta birey (200-350g) incelendi. Hasta balık örneklerinde solgun solungaç ve karaciğer, deri renginde açılma, karaciğerde ve kaslarda kanamalar görüldü. Hasta balıkların karaciğer, böbrek ve dalağından Marine Agar (MA) ve % 1,5 NaCl içeren Tryptic Soy Agar (TSA) yapılan bakteriyolojik ekimler 22 ° C'de 48 saat süreyle inkübe edildi. Gram pozitif hareketli olmayan koklar viseral organlardan izole edildi. Morfolojik ve biyokimyasal özelliklerine göre, bu izolatlar Miccrococcus luteus olarak identifiye edildi. Kirby-Bauer disk difüzyon yöntemi çoklu disk kullanılarak yapıldı. Sonuçlar mevcut verilerine dayanılarak yorumlandı ve tüm izolatların oksitetrasiklin, eritromisin ve kloramfenikole karşı duyarlı olduğu belirlendi. Histopatolojik olarak karaciğer parankim hücrelerinde vakuolar dejenerasyon ve nekroz, böbreklerde tübüler nekroz ve dalakta hemosiderin depozitleri saptandı. Lamellalarda erimenin yanı sıra solungaçlarda epitel hücre hiperplazisi gözlendi. Sonuç olarak, bu çalışmada M. luteus’un neden olduğu bir düşük mortaliteli bir epizootik tanımlandı ve bu çalışma bu patojenin kültür sivriburun karagöz balıklarında tanımlandığı ilk rapordur.

Keywords

• Sivriburun Karagöz,Kafes Yetiştiriciliği,Micrococcus luteus

Diagnosis of Micrococcus luteus Infection in Cultured Sharpsnout Sea Bream (Diplodus puntazzo Cetti, 1777)

Abstract

The aim of the study was to determinate the cause of mortalities in cultured sharpsnout sea bream (Diplodus puntazzo Cetti, 1777) in the Aegean Sea. Ten individuals (200-350 g) in total between May 2009 – 2010 were investigated using bacteriological methods. Moribund fish samples exhibited pale gills and liver, skin depigmentation, haemorrhaging in the liver and muscles. Bacteriological samples of liver, kidney and spleen were streaked onto Marine Agar (MA) and Tryptic Soy Agar (TSA) plates containing %1,5 NaCl and incubated at 22°C for 48h. Gram-positive non-motile cocci were isolated from visceral organs. According to their morphological and biochemical characteristics, these isolates were identified as Micrococcus luteus. The Kirby-Bauer disk diffusion method was also performed using multidisc. Results were interpreted based on the available data and all isolates were determined to be sensitive against oxytetracycline, erythromycin and chloramphenicol. Histopathologically, vacuolar degeneration and necrosis in the parenchyma cells of liver, tubular necrosis in the kidney and hemosiderin deposits in the spleen were determined. In addition to melting lamella, epithelial cells hyperplasia in the gills was observed. In conclusion, in this study an epizootic with low mortality caused M. luteus were described and this study is the first report of the pathogen in the cultured sharpsnout sea bream.

Keywords

• Sharpsnout Sea Bream,Cage Culture,Micrococcus luteus

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