<?xml version="1.0" encoding="UTF-8"?>
<!DOCTYPE article PUBLIC "-//NLM//DTD JATS (Z39.96) Journal Publishing DTD v1.4 20241031//EN"
        "https://jats.nlm.nih.gov/publishing/1.4/JATS-journalpublishing1-4.dtd">
<article  article-type="research-article"        dtd-version="1.4">
            <front>

                <journal-meta>
                                                                <journal-id>ert</journal-id>
            <journal-title-group>
                                                                                    <journal-title>Environmental Research and Technology</journal-title>
            </journal-title-group>
                                        <issn pub-type="epub">2636-8498</issn>
                                                                                            <publisher>
                    <publisher-name>Mehmet Sinan Bilgili</publisher-name>
                </publisher>
                    </journal-meta>
                <article-meta>
                                        <article-id pub-id-type="doi">10.35208/ert.1062683</article-id>
                                                                <article-categories>
                                            <subj-group  xml:lang="en">
                                                            <subject>Environmental Sciences</subject>
                                                            <subject>Water Resources and Water Structures</subject>
                                                            <subject>Public Health</subject>
                                                            <subject>Environmental Health</subject>
                                                    </subj-group>
                                            <subj-group  xml:lang="tr">
                                                            <subject>Çevre Bilimleri</subject>
                                                            <subject>Su Kaynakları ve Su Yapıları</subject>
                                                            <subject>Halk Sağlığı</subject>
                                                            <subject>Çevre Sağlığı</subject>
                                                    </subj-group>
                                    </article-categories>
                                                                                                                                                        <title-group>
                                                                                                                        <article-title>Principal component analysis in long term assessment of total viable plate count of municipal water distribution network system in healthcare facility</article-title>
                                                                                                                                        </title-group>
            
                                                    <contrib-group content-type="authors">
                                                                        <contrib contrib-type="author">
                                                                    <contrib-id contrib-id-type="orcid">
                                        https://orcid.org/0000-0003-3562-5935</contrib-id>
                                                                <name>
                                    <surname>Eissa</surname>
                                    <given-names>Mostafa</given-names>
                                </name>
                                                            </contrib>
                                                    <contrib contrib-type="author">
                                                                    <contrib-id contrib-id-type="orcid">
                                        https://orcid.org/0000-0002-6593-378X</contrib-id>
                                                                <name>
                                    <surname>Rashed</surname>
                                    <given-names>Engy</given-names>
                                </name>
                                                            </contrib>
                                                    <contrib contrib-type="author">
                                                                    <contrib-id contrib-id-type="orcid">
                                        https://orcid.org/0000-0002-6340-8973</contrib-id>
                                                                <name>
                                    <surname>Eissa</surname>
                                    <given-names>Dalia</given-names>
                                </name>
                                                            </contrib>
                                                                                </contrib-group>
                        
                                        <pub-date pub-type="pub" iso-8601-date="20220630">
                    <day>06</day>
                    <month>30</month>
                    <year>2022</year>
                </pub-date>
                                        <volume>5</volume>
                                        <issue>2</issue>
                                        <fpage>165</fpage>
                                        <lpage>171</lpage>
                        
                        <history>
                                    <date date-type="received" iso-8601-date="20220125">
                        <day>01</day>
                        <month>25</month>
                        <year>2022</year>
                    </date>
                                                    <date date-type="accepted" iso-8601-date="20220427">
                        <day>04</day>
                        <month>27</month>
                        <year>2022</year>
                    </date>
                            </history>
                                        <permissions>
                    <copyright-statement>Copyright © 2018, Environmental Research and Technology</copyright-statement>
                    <copyright-year>2018</copyright-year>
                    <copyright-holder>Environmental Research and Technology</copyright-holder>
                </permissions>
            
                                                                                                <abstract><p>The microbial quality of water is a critical safety aspect that should not be overlooked, especially when considering human consumption in the facilities for healthcare and the treatment of health compromised populations. The present work aimed to study a municipal network for city water within a healthcare facility microbiologically. The implementation of multivariate analysis was conducted over collected data to decipher trends of the microbiological count of samples from different points-of-use. The statistical study involved an Eigenvalue determination, score graph, loading plot study and outlier diagram. Data showed clustering tendency with aberrant values could be observed. The first component appeared to be associated with bioburden density in the water. While the other component showed a link to the relative locations of the distribution points in the facility and the length of the water lines from the source feeding city water to a great extent. The two components explained more than 55% of the variations in data. A property that highlighted a strong agreement between the order of points-of-use in the loading plot with that of ratio factor which was obtained from the Total Viable Count over the hypothetical distance of sampling port from the reference feeding entrance. Thus, there was evidence of variation in the microbial quality affected by the biological stability of the water distribution system that needed attention and an improvement plan for better control of microbial content in city water to prevent possible excursions in the future. The study showed a valuable perspective approach for the quantitative examination of the microbiological stability of the municipal water distribution network system.</p></abstract>
                                                                                    
            
                                                            <kwd-group>
                                                    <kwd>Eigen value</kwd>
                                                    <kwd>  loading plot</kwd>
                                                    <kwd>  outlier diagram</kwd>
                                                    <kwd>  PCA</kwd>
                                                    <kwd>  score graph</kwd>
                                                    <kwd>  scree plot</kwd>
                                            </kwd-group>
                                                        
                                                                                                                                                    </article-meta>
    </front>
    <back>
                            <ref-list>
                                    <ref id="ref1">
                        <label>1</label>
                        <mixed-citation publication-type="journal">[1].	M. D. Sobsey, C. E. Stauber, L. M. Casanova, J. M. Brown, and M. A. Elliott, “Point of use household drinking water filtration: A practical, effective solution for providing sustained access to safe drinking water in the developing world,” Environ. Sci. Technol., vol. 42 (12), pp. 4261–4267, 2008.</mixed-citation>
                    </ref>
                                    <ref id="ref2">
                        <label>2</label>
                        <mixed-citation publication-type="journal">[2]. G. Ducel, J. Fabry and L. Nicolle, Prevention of hospital-acquired infections, 2nd ed. World Health Organization, Geneva, 2002.</mixed-citation>
                    </ref>
                                    <ref id="ref3">
                        <label>3</label>
                        <mixed-citation publication-type="journal">[3].  V. Gauthier, B. Gérard, J. Portal, J. Block and D. Gatel, &quot;Organic matter as loose deposits in a drinking water distribution system&quot;, Water Research, vol. 33 (4), pp. 1014-1026, 1999. Available: 10.1016/s0043-1354(98)00300-5.</mixed-citation>
                    </ref>
                                    <ref id="ref4">
                        <label>4</label>
                        <mixed-citation publication-type="journal">[4]. World Health Organization (WHO), &quot;Potable Reuse: Guidance for producing safe drinking-water&quot;, Apps.who.int, 2017. [Online]. Available: https://apps.who.int/iris/bitstream/handle/10665/258715/9789241512770-eng.pdf. [Accessed: 5 January 2022].</mixed-citation>
                    </ref>
                                    <ref id="ref5">
                        <label>5</label>
                        <mixed-citation publication-type="journal">[5]. R. Saha and R. Donofrio, &quot;The microbiology of metalworking fluids&quot;, Applied Microbiology and Biotechnology, vol. 94 (5), pp. 1119-1130, 2012. Available: 10.1007/s00253-012-4055-7 [Accessed 5 January 2022].</mixed-citation>
                    </ref>
                                    <ref id="ref6">
                        <label>6</label>
                        <mixed-citation publication-type="journal">[6]. M. EISSA, &quot;Assessment for Sporicidal Activity of Two Types of Peroxygen/Silver-Based Disinfectants: A Comparative Study&quot;, Journal of Pharmaceutical Technology, Research and Management, vol. 4 (2), pp. 161-169, 2016. Available: 10.15415/jptrm.2016.42011 [Accessed 5 January 2022].</mixed-citation>
                    </ref>
                                    <ref id="ref7">
                        <label>7</label>
                        <mixed-citation publication-type="journal">[7]. Great Learning Team, &quot;Overview of Multivariate Analysis | What is Multivariate Analysis and Model Building Process?&quot;, GreatLearning Blog: Free Resources what Matters to shape your Career!, 2020. [Online]. Available: https://www.mygreatlearning.com/blog/introduction-to-multivariate-analysis/. [Accessed: 5 January 2022].</mixed-citation>
                    </ref>
                                    <ref id="ref8">
                        <label>8</label>
                        <mixed-citation publication-type="journal">[8]. R. Ainsworth, &quot;Safe Piped Water: Managing Microbial Water Quality in Piped Distribution Systems&quot;, Water Intelligence Online, vol. 12, 2013. Available: 10.2166/9781780405841.</mixed-citation>
                    </ref>
                                    <ref id="ref9">
                        <label>9</label>
                        <mixed-citation publication-type="journal">[9]. M. Essam Eissa, &quot;Investigation of Microbiological Quality of Water from the Feed Source to the Terminal Application in the Healthcare Facility: A Case Study&quot;, Health Research, vol. 2 (1), pp. 16, 2018. Available: 10.31058/j.hr.2018.21002 [Accessed 19 January 2022].</mixed-citation>
                    </ref>
                                    <ref id="ref10">
                        <label>10</label>
                        <mixed-citation publication-type="journal">[10].EMSL Analytical, Inc, &quot;Legionella, Fecal Coliform &amp; Total Coliform Sampling Guide&quot;, Emsl.com, 2022. [Online]. Available: https://www.emsl.com/PDFDocuments/SamplingGuide/legionella_coliform_guide.pdf. [Accessed: 16- Jan- 2022].</mixed-citation>
                    </ref>
                                    <ref id="ref11">
                        <label>11</label>
                        <mixed-citation publication-type="journal">[11].United States Environmental Protection Agency, &quot;Quick Guide To Drinking Water Sample Collection&quot;, Epa.gov, 2022. [Online]. Available: https://www.epa.gov/sites/default/files/2015-11/documents/drinking_water_sample_ collection. pdf. [Accessed: 15- Jan- 2022].</mixed-citation>
                    </ref>
                                    <ref id="ref12">
                        <label>12</label>
                        <mixed-citation publication-type="journal">[12].D. Francy, E.A. Stelzer, A.M. Brady, C. Huitger, R.N. Bushon, H.S. Ip, M.W. Ware, E.N. Villegas, V. Gallardo and H.D. Lindquist, &quot;Comparison of Filters for Concentrating Microbial Indicators and Pathogens in Lake Water Samples&quot;, Applied and Environmental Microbiology, vol. 79 (4), pp. 1342-1352, 2012. Available: 10.1128/aem.03117-12.</mixed-citation>
                    </ref>
                                    <ref id="ref13">
                        <label>13</label>
                        <mixed-citation publication-type="journal">[13].G. Ngwa, R. Schop, S. Weir, C. León-Velarde and J. Odumeru, &quot;Detection and enumeration of E. coli O157:H7 in water samples by culture and molecular methods&quot;, Journal of Microbiological Methods, vol. 92 (2), pp. 164-172, 2013. Available: 10.1016/j.mimet.2012.11.018 [Accessed 17 January 2022].</mixed-citation>
                    </ref>
                                    <ref id="ref14">
                        <label>14</label>
                        <mixed-citation publication-type="journal">[14].E. Goldwater, &quot;Using Excel for Data Analysis&quot;, People.umass.edu, 2022. [Online]. Available: https://people.umass.edu/evagold/excel.html. [Accessed: 19- Jan- 2022].</mixed-citation>
                    </ref>
                                    <ref id="ref15">
                        <label>15</label>
                        <mixed-citation publication-type="journal">[15].H. Motulsky, &quot;Home - GraphPad&quot;, Graphpad.com, 2022. [Online]. Available: https://www.graphpad.com/. [Accessed: 18- Jan- 2022].</mixed-citation>
                    </ref>
                                    <ref id="ref16">
                        <label>16</label>
                        <mixed-citation publication-type="journal">[16]. M. Essam Eissa, &quot;Determination of the Microbiological Quality of Feed City Water to Pharmaceutical Facility: Distribution Study and Statistical Analysis&quot;, ATHENS JOURNAL OF SCIENCES, vol. 4 (2), pp. 143-160, 2017. Available: 10.30958/ajs.4-2-4 [Accessed 19 January 2022].</mixed-citation>
                    </ref>
                                    <ref id="ref17">
                        <label>17</label>
                        <mixed-citation publication-type="journal">[17].M. Eissa, “Evaluation of microbiological purified water trend using two types of control chart”, European Pharmaceutical Review, vol. 23 (5), pp. 36-38, 2018.</mixed-citation>
                    </ref>
                                    <ref id="ref18">
                        <label>18</label>
                        <mixed-citation publication-type="journal">[18].E. Bassett, S. Brooks and B. Morgan, &quot;MINITAB Multivariate Macros&quot;, Applied Statistics, vol. 45 (2), p. 235, 1996. Available: 10.2307/2986157 [Accessed 12 January 2022].</mixed-citation>
                    </ref>
                                    <ref id="ref19">
                        <label>19</label>
                        <mixed-citation publication-type="journal">[19].L. Almagro, X. Llabres and P. Cintas, Industrial statistics with minitab. Hoboken, N.J.: Wiley, 2013.</mixed-citation>
                    </ref>
                                    <ref id="ref20">
                        <label>20</label>
                        <mixed-citation publication-type="journal">[20].T. Cox, An introduction to multivariate data analysis. London: Hodder Arnold, 2005.</mixed-citation>
                    </ref>
                                    <ref id="ref21">
                        <label>21</label>
                        <mixed-citation publication-type="journal">[21].Minitab® 18 Support. 2019, &quot;Interpret all statistics and graphs for Factor Analysis - Minitab&quot;, Support.minitab.com, 2022. [Online]. Available: https://support.minitab.com/en-us/minitab/18/help-and-how-to/modeling-statistics/multivariate/how-to/factor-analysis/interpret-the-results/all-statistics-and-graphs/. [Accessed: 12- Jan- 2022].</mixed-citation>
                    </ref>
                                    <ref id="ref22">
                        <label>22</label>
                        <mixed-citation publication-type="journal">[22].D. Weragama, V. Weerasingha, L. Jayasumana, J. Adikari, J. Vidanarachchi and H. Priyashantha, &quot;The physicochemical, microbiological, and organoleptic properties and antioxidant activities of cream cheeses fortified with dried curry leaves ( Murraya koenigii L.) powder&quot;, Food Science &amp; Nutrition, vol. 9 (10), pp. 5774-5784, 2021. Available: 10.1002/fsn3.2551.</mixed-citation>
                    </ref>
                                    <ref id="ref23">
                        <label>23</label>
                        <mixed-citation publication-type="journal">[23].GraphPad Software LLC, &quot;GraphPad Prism 9 Statistics Guide - How to: Correlation&quot;, Graphpad.com, 2022. [Online]. Available: https://www.graphpad.com/guides/prism/latest/statistics/stat_how_to_correlation.htm. [Accessed: 13- Jan- 2022].</mixed-citation>
                    </ref>
                                    <ref id="ref24">
                        <label>24</label>
                        <mixed-citation publication-type="journal">[24].C. Charoux, L. Free, L.M. Hinds, R.K. Vijayaraghavan, S. Daniels, C.P. O&#039;Donnell and B.K. Tiwari, &quot;Effect of non-thermal plasma technology on microbial inactivation and total phenolic content of a model liquid food system and black pepper grains&quot;, LWT, vol. 118, pp. 108716, 2020. Available: 10.1016/j.lwt.2019.108716.</mixed-citation>
                    </ref>
                                    <ref id="ref25">
                        <label>25</label>
                        <mixed-citation publication-type="journal">[25].M.E. Eissa, “Role of Statistical Process Control of Pharmaceutical Product to Monitor Consistency of the Manufacturing Operation”, EC Pharmacology and Toxicology, vol. 6, pp. 439-44, 2018.</mixed-citation>
                    </ref>
                            </ref-list>
                    </back>
    </article>
